公开(公告)号：US20200263160A1

公开(公告)日：2020-08-20

申请号：US16865487

申请日：2020-05-04

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Werner Hoelke ,  Michaela Fischer ,  Johann-Peter Thalhofer ,  Markus Weber

IPC: C12N9/96 ,  C12N9/52 ,  C12N9/64 ,  C12N9/54

Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.

公开(公告)号：US20160319268A1

公开(公告)日：2016-11-03

申请号：US14699299

申请日：2015-04-29

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Werner Hoelke ,  Michaela Fischer ,  Johann-Peter Thalhofer ,  Markus Weber

IPC: C12N9/96 ,  C12N9/54 ,  C12N9/52

CPC classification number: C12N9/96 ,  C12N9/52 ,  C12N9/54 ,  C12N9/6491 ,  C12Y304/24003 ,  C12Y304/24027

Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.

Abstract translation: 描述了含有蛋白水解酶的组合物，特别是冻干物，以及用于制备组合物的方法。 通常，这些组合物含有一种或多种具有胶原酶活性的蛋白酶和中性蛋白酶，例如嗜热菌蛋白酶。 组合物不含乙酸盐。 令人惊奇的是，这种组合物可以比现有技术的冻干蛋白酶混合物更快地溶解在水中。

公开(公告)号：US20160010069A1

公开(公告)日：2016-01-14

申请号：US14872392

申请日：2015-10-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Greif ,  Christian Rudolph ,  Manfred Schmidt ,  Harald Sobek ,  Johann-Peter Thalhofer

IPC: C12N9/12

CPC classification number: C12N9/1247 ,  C12Y207/07006

Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.

Abstract translation: 本公开提供了T7RNA聚合酶的新变体。 根据本发明的T7变体的实施方案包括T7多肽的氨基酸序列的位置723上的半胱氨酸 - 丝氨酸取代。 根据本发明的T7变体的实施方案具有DNA依赖性RNA聚合酶酶活性，并且通过氧化硫醇基形成分子内同型二聚体的趋势降低。 本文公开的T7变体内的氨基酸取代最小程度地影响T7多肽的RNA聚合酶活性。 此外，所公开的实施方案的突变可以任选地与与野生型参考物相比提供增强的热稳定性的突变组合。