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    Multi-epitope fusion protein of an HCV antigen and uses thereof
    11.
    发明授权
    Multi-epitope fusion protein of an HCV antigen and uses thereof 有权

    公开(公告)号：US11078241B2

    公开(公告)日：2021-08-03

    申请号：US16751609

    申请日：2020-01-24

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Barbara Upmeier ,  Ralf Bollhagen ,  Toralf Zarnt

    IPC: C07K14/005 ,  G01N33/576

    Abstract: The disclosure relates to a multi-epitope fusion protein as well as to its use as calibrator and/or control in an in vitro diagnostics immunoassay for detecting HCV core antigen. The multi-epitope fusion protein has two to six different non-overlapping linear peptides present in the amino acid sequence of hepatitis C virus (HCV) core protein, wherein each of the peptides is separated from the other peptides by a spacer consisting of a non-HCV amino acid sequence and having a chaperone amino acid sequence. No further HCV specific amino acid sequences are present in the polypeptide. A further aspect relates to a reagent kit for detecting HCV core antigen containing said multi-epitope fusion protein as calibrator or control or both.

    Methods for reducing interferences
    14.
    发明授权
    Methods for reducing interferences 有权

    公开(公告)号：US11156610B2

    公开(公告)日：2021-10-26

    申请号：US15621280

    申请日：2017-06-13

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Barbara Upmeier ,  Toralf Zarnt ,  Johannes Polz

    IPC: G01N31/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

    Abstract: Described is a method for determining an analyte in a sample suspected to contain the analyte, by a) contacting the sample with a first and a second capture compound for the analyte, wherein the first and second capture compounds are non-identical capture compounds, and the capture compounds compete in binding to the analyte; b) contacting the capture compounds contacted with the sample with a specifier, wherein the specifier competes in binding to the capture compounds with the analyte; c) determining the amount of complexes having the specifier and a capture compound; and d) determining the analyte in a sample based on the result of step c). Also disclosed is a method for improving the specificity of an indirect immunoassay for determining an analyte, as well as kits, devices, and uses related to the methods.

    METHODS FOR REDUCING INTERFERENCES
    17.
    发明申请
    METHODS FOR REDUCING INTERFERENCES 审中-公开

    公开(公告)号：US20180128825A1

    公开(公告)日：2018-05-10

    申请号：US15621280

    申请日：2017-06-13

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Barbara Upmeier ,  Toralf Zarnt ,  Johannes Polz

    IPC: G01N33/543 ,  G01N33/68

    CPC classification number: G01N33/54393 ,  G01N33/54306 ,  G01N33/6854 ,  G01N2800/26

    Abstract: The present invention relates to a method for determining an analyte in a sample suspected to comprise said analyte, comprising a) contacting with said sample at least a first and a second capture compound for said analyte, wherein said first and second capture compounds are non-identical capture compounds, and wherein said capture compounds compete in binding to said analyte; b) contacting said capture compounds contacted with said sample with a specifier, wherein said specifier competes in binding to said capture compounds with said analyte; c) determining the amount of complexes comprising said specifier and a capture compound; and d) determining said analyte in a sample based on the result of step c). The present invention further relates to a method for improving the specificity of an indirect immunoassay for determining an analyte, comprising replacing at least 10% of a capture compound by a non-identical capture compound; wherein the capture compound replaced competes in binding to said analyte with the capture compound introduced. The present invention further relates to kits, devices, and uses related to the aforementioned methods.

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