公开(公告)号：US20150291985A1

公开(公告)日：2015-10-15

申请号：US14439834

申请日：2013-09-20

Applicant: SHOWA DENKO K.K.

Inventor： Hirobumi Aoki ,  Yuzuru Kokido ,  Yoko Hashimoto ,  Tadashi Yoneda

IPC: C12P7/18 ,  C12N9/04

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y402/0112 ,  C12Y503/03003

Abstract: A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.

Abstract translation: 通过3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A的酶反应体系依次使用微生物和/或其培养物制备1,4-丁二醇的方法，其中3-羟基丁酰基 - CoA是光学活性物质，其中微生物包括（1）编码烯酰辅酶A水合酶的基因，（2）编码乙酰乙酰辅酶Aδ异构酶的基因，（3）编码4-羟基丁酰辅酶A脱水酶的基因 ，（4）编码酰基辅酶A还原酶的基因，其底物特异性具有与3-羟基丁酰辅酶A相反的光学选择性。

公开(公告)号：US20150232830A1

公开(公告)日：2015-08-20

申请号：US14429459

申请日：2013-09-19

Applicant: SHOWA DENKO K.K.

Inventor： Hirobumi Aoki ,  Yuzuru Kokido ,  Tadashi Yoneda

IPC: C12N9/88 ,  C12P7/18 ,  C12P19/32

CPC classification number: C12N9/88 ,  C12P7/16 ,  C12P7/18 ,  C12P19/32 ,  C12Y402/01017 ,  Y02E50/10

Abstract: A gene is selected from the group consisting of a gene (a) that has a base sequence of sequence number 1, a gene (b) that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene (b) has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and a gene (c) that hybridizes with a gene that has a base sequence complimentary with a gene that has a base sequence of sequence number 1 on a stringent condition, wherein the selected gene in combination with a gene that codes enoyl-CoA hydratase is able to provide a microbe or a culture of the microbe with an ability to convert 3-hydroxybutyryl-CoA into 4-hydroxybutyryl-CoA or 4-hydroxybutyryl-CoA into 3-hydroxybutyryl-CoA.

Abstract translation: 基因选自具有序列号1的碱基序列的基因（a），具有碱基序列的基因（b），使得一个或多个碱基被缺失，取代或加入到碱基中 序列号1的序列，其中基因（b）具有相对于序列号1的碱基序列具有大于或等于90％的同一性的碱基序列，以及与具有 与在严格条件下具有序列号1的碱基序列的基因互补的碱基序列，其中所选择的基因与编码烯酰辅酶A水合酶的基因组合能够提供微生物或微生物的培养物 将3-羟基丁酰辅酶A转化为4-羟基丁酰辅酶A或4-羟基丁酰辅酶A的能力转化为3-羟基丁酰辅酶A。