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公开(公告)号：US20150275236A1
公开(公告)日：2015-10-01
申请号：US14438292
申请日：2013-09-13
Applicant: SHOWA DENKO K.K.
Inventor： Hirobumi Aoki , Yuzuru Kokido , Yoko Hashimoto , Tadashi Yoneda
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01035 , C12Y101/01036 , C12Y101/01157 , C12Y102/0101 , C12Y402/01017 , C12Y402/01055 , C12Y402/01119 , C12Y402/0112 , C12Y503/03003
Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.
Abstract translation: 通过使用乙酰乙酰辅酶A还原酶和烯酰辅酶A水合酶的酶反应体系，通过乙酰乙酰辅酶A，3-羟基丁酰辅酶A和巴豆酰基-CoA的方法，使用微生物和/或其培养物制备1,4-丁二醇的方法， CoA，其中每个乙酰乙酰辅酶A还原酶和烯酰辅酶A水合酶对3-羟基丁酰辅酶A的立体异构体是特异性的。
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公开(公告)号：US09677096B2
公开(公告)日：2017-06-13
申请号：US14440116
申请日：2013-11-28
Applicant: SHOWA DENKO K.K.
Inventor： Hirobumi Aoki , Yuzuru Kokido , Yoko Hashimoto , Tadashi Yoneda
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/1029 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01035 , C12Y101/01036 , C12Y101/01157 , C12Y203/01009 , C12Y402/01017 , C12Y402/01055 , C12Y402/01119 , C12Y402/0112 , C12Y503/03003
Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.
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公开(公告)号：US20150291985A1
公开(公告)日：2015-10-15
申请号：US14439834
申请日：2013-09-20
Applicant: SHOWA DENKO K.K.
Inventor： Hirobumi Aoki , Yuzuru Kokido , Yoko Hashimoto , Tadashi Yoneda
CPC classification number: C12P7/18 , C12N9/0006 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01036 , C12Y101/01157 , C12Y402/0112 , C12Y503/03003
Abstract: A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.
Abstract translation: 通过3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A的酶反应体系依次使用微生物和/或其培养物制备1,4-丁二醇的方法，其中3-羟基丁酰基 - CoA是光学活性物质，其中微生物包括（1）编码烯酰辅酶A水合酶的基因，（2）编码乙酰乙酰辅酶Aδ异构酶的基因，（3）编码4-羟基丁酰辅酶A脱水酶的基因 ，（4）编码酰基辅酶A还原酶的基因，其底物特异性具有与3-羟基丁酰辅酶A相反的光学选择性。
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