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    Method for comparison of DNA base sequences
    11.
    发明授权
    Method for comparison of DNA base sequences 失效
    DNA碱基序列比较方法

    公开(公告)号：US06662115B2

    公开(公告)日：2003-12-09

    申请号：US09050010

    申请日：1998-03-30

    申请人： Keiichi Nagai ,  Ryotaro Irie ,  Susumu Hiraoka ,  Naoko Kasahara

    发明人： Keiichi Nagai ,  Ryotaro Irie ,  Susumu Hiraoka ,  Naoko Kasahara

    IPC分类号： G06F1900

    CPC分类号： G06F19/22

    摘要： A method for comparing DNA base sequences by comparing similarities between two amino acid sequences translated from two DNA base sequences, respectively, includes (1) a step of dividing each of the first DNA base sequence and the second DNA base sequence into groups of successive three nucleotides each, translating each of these groups of nucleotides into an amino acid, and thereby obtaining a first amino acid sequence and a second amino acid sequence, and (2) a step of determining similarities between each amino acid of the first translated amino acid sequence and each amino acid of the second translated amino acid sequence in view of nucleotide insertions or deletions in the first and second DNA base sequence and amino acid insertions or deletions in the first and second translated amino acid sequence. The method is repeated by shifting the base sequences one base at a time.

    摘要翻译： 通过比较分别从两个DNA碱基序列翻译的两个氨基酸序列之间的相似性来比较DNA碱基序列的方法包括（1）将第一DNA碱基序列和第二DNA碱基序列中的每一个分成连续三个组的步骤 核苷酸各自将这些核苷酸组中的每一个转化成氨基酸，从而获得第一氨基酸序列和第二氨基酸序列，和（2）确定第一翻译氨基酸序列的每个氨基酸之间的相似性的步骤 考虑到第一和第二DNA碱基序列中的核苷酸插入或缺失以及第一和第二翻译氨基酸序列中的氨基酸插入或缺失，第二翻译氨基酸序列的每个氨基酸。 通过一次移位一个碱基来重复该方法。

    NOVEL GUANOSINE TRIPHOSPHATE-BINDING PROTEIN-COUPLED RECEPTOR PLACE6002312, ITS GENE, AND PRODUCTION AND USES THEREOF
    13.
    发明申请
    NOVEL GUANOSINE TRIPHOSPHATE-BINDING PROTEIN-COUPLED RECEPTOR PLACE6002312, ITS GENE, AND PRODUCTION AND USES THEREOF 审中-公开
    新型鸟嘌呤三磷酸结合蛋白偶联受体PLACE6002312，其基因及其生产及其用途

    公开(公告)号：US20080171353A1

    公开(公告)日：2008-07-17

    申请号：US11834900

    申请日：2007-08-07

    申请人： Tomoyasu SUGIYAMA ,  Noriyuki Morikawa ,  Ai Wakamatsu ,  Tamaki Oda ,  Ryotaro Irie ,  Takao Isogai ,  Yasuhiko Masuho

    发明人： Tomoyasu SUGIYAMA ,  Noriyuki Morikawa ,  Ai Wakamatsu ,  Tamaki Oda ,  Ryotaro Irie ,  Takao Isogai ,  Yasuhiko Masuho

    IPC分类号： C12Q1/02

    CPC分类号： C07K14/705 ,  G01N2333/4719 ,  G01N2500/00

    摘要： The clone PLACE6002312 having a structure characteristic of G protein-coupled receptor was isolated from a human placental full-length cDNA library prepared by the oligo-capping method. Database search revealed that PLACE6002312 had the highest homology to HISTAMINE H2 RECEPTOR. Analysis for the expression of PLACE6002312 gene in human tissues revealed that the gene was expressed in various tissues, such as heart, liver, and ovary. In addition, histamine was found to be one of ligands for PLACE 6002312 by ligand-binding assay. Furthermore, a full-length cDNA for the mouse homolog of PLACE6002312 was isolated and the deduced amino acid sequence was revealed to comprise a structure characteristic of G protein-coupled receptor. PLACE6002312 can be used to screen for agonists and antagonists useful as pharmaceuticals and to diagnose various diseases caused by the abnormal activity or expression of the polypeptide.

    摘要翻译： 从通过寡聚体封端法制备的人胎盘全长cDNA文库中分离出具有G蛋白偶联受体结构特征的克隆PLACE6002312。 数据库检索显示PLACE6002312与HISTAMINE H2受体的同源性最高。 对人类组织中PLACE6002312基因表达的分析显示，该基因在各种组织中表达，如心脏，肝脏和卵巢。 此外，通过配体结合测定，发现组胺是PLACE 6002312的配体之一。 此外，分离出PLACE6002312的小鼠同源物的全长cDNA，并推测出氨基酸序列包含G蛋白偶联受体的结构特征。 PLACE6002312可用于筛选可用作药物的激动剂和拮抗剂，并诊断由多肽的异常活性或表达引起的各种疾病。