公开(公告)号：US20080171353A1

公开(公告)日：2008-07-17

申请号：US11834900

申请日：2007-08-07

申请人： Tomoyasu SUGIYAMA ,  Noriyuki Morikawa ,  Ai Wakamatsu ,  Tamaki Oda ,  Ryotaro Irie ,  Takao Isogai ,  Yasuhiko Masuho

发明人： Tomoyasu SUGIYAMA ,  Noriyuki Morikawa ,  Ai Wakamatsu ,  Tamaki Oda ,  Ryotaro Irie ,  Takao Isogai ,  Yasuhiko Masuho

IPC分类号： C12Q1/02

CPC分类号： C07K14/705 ,  G01N2333/4719 ,  G01N2500/00

摘要： The clone PLACE6002312 having a structure characteristic of G protein-coupled receptor was isolated from a human placental full-length cDNA library prepared by the oligo-capping method. Database search revealed that PLACE6002312 had the highest homology to HISTAMINE H2 RECEPTOR. Analysis for the expression of PLACE6002312 gene in human tissues revealed that the gene was expressed in various tissues, such as heart, liver, and ovary. In addition, histamine was found to be one of ligands for PLACE 6002312 by ligand-binding assay. Furthermore, a full-length cDNA for the mouse homolog of PLACE6002312 was isolated and the deduced amino acid sequence was revealed to comprise a structure characteristic of G protein-coupled receptor. PLACE6002312 can be used to screen for agonists and antagonists useful as pharmaceuticals and to diagnose various diseases caused by the abnormal activity or expression of the polypeptide.

摘要翻译： 从通过寡聚体封端法制备的人胎盘全长cDNA文库中分离出具有G蛋白偶联受体结构特征的克隆PLACE6002312。 数据库检索显示PLACE6002312与HISTAMINE H2受体的同源性最高。 对人类组织中PLACE6002312基因表达的分析显示，该基因在各种组织中表达，如心脏，肝脏和卵巢。 此外，通过配体结合测定，发现组胺是PLACE 6002312的配体之一。 此外，分离出PLACE6002312的小鼠同源物的全长cDNA，并推测出氨基酸序列包含G蛋白偶联受体的结构特征。 PLACE6002312可用于筛选可用作药物的激动剂和拮抗剂，并诊断由多肽的异常活性或表达引起的各种疾病。

公开(公告)号：US07402435B2

公开(公告)日：2008-07-22

申请号：US10470732

申请日：2002-02-08

申请人： Sousuke Miyoshi ,  Akiko Ohkubo ,  Noriyuki Morikawa ,  Yasuhiro Ogawa ,  Shintaro Nishimura ,  Masao Fukagawa ,  Hiroyuki Arakawa ,  Jyunko Zenkou ,  Susumu Sato

发明人： Sousuke Miyoshi ,  Akiko Ohkubo ,  Noriyuki Morikawa ,  Yasuhiro Ogawa ,  Shintaro Nishimura ,  Masao Fukagawa ,  Hiroyuki Arakawa ,  Jyunko Zenkou ,  Susumu Sato

IPC分类号： C12N15/00 ,  C12N15/02

CPC分类号： C12N15/87 ,  C12M35/02 ,  C12N15/02

摘要： A method of highly efficiently transferring various selected molecules into various cells and a method of fusing cells. Cells and/or selected molecules such as polynucleotide are treated with cold gas plasma to thereby transfer the selected molecules located around cells into the cells, or cells are fused by treating the cells with cold gas plasma. Moreover, an apparatus for transferring selected molecules or fusing cells having a cold gas plasma generation unit for transferring selected molecules into cells is provided.

摘要翻译： 将各种选择的分子高效转移到各种细胞中的方法和融合细胞的方法。 用冷气体等离子体处理细胞和/或选择的分子，例如多核苷酸，从而将位于细胞周围的选定分子转移到细胞中，或者通过用冷气体等离子体处理细胞来融合细胞。 此外，提供了一种用于转移所选分子或具有用于将选定分子转移到细胞中的具有冷气体等离子体产生单元的融合电池的装置。

公开(公告)号：US08741969B2

公开(公告)日：2014-06-03

申请号：US11989701

申请日：2006-08-02

申请人： Hitoshi Otani ,  Shojiro Matsuda ,  Tsuguyoshi Taira ,  Noriyuki Morikawa

发明人： Hitoshi Otani ,  Shojiro Matsuda ,  Tsuguyoshi Taira ,  Noriyuki Morikawa

IPC分类号： A61K47/42 ,  C07K1/00 ,  C07K14/78 ,  A61P19/04

CPC分类号： A61K47/42 ,  A61L31/045 ,  C07K1/00 ,  C07K14/78

摘要： An object of the present invention is to provide an anti-adhesion membrane that has no toxicity to a living body, has flexibility allowing itself to fit an affected part as a hydrated gel, is uniformly crosslinked, and is immediately absorbed in a living body after maintaining its shape in the living body for a certain period of time.The present invention provides anti-adhesion material, which comprises a thermally crosslinked gelatin film, and has a water content of 60 to 85% calculated by the following formula (1): water content (%)=[(Ws−Wd)/Ws]×100(%)  (1), in the formula (1), Ws representing a weight (wet weight) of the anti-adhesion material immersed in a phosphate buffered saline solution at a temperature of 25° C. for one hour, and Wd representing a weight (dry weight) of the anti-adhesion material dried completely using a vacuum drying apparatus.

摘要翻译： 本发明的目的是提供一种对生物体无毒性的抗粘附膜，具有使其自身适合作为水合凝胶的患部的柔软性，均匀交联，并且立即被吸收在生物体内 保持其在生命体中的形状一段时间。 本发明提供一种抗粘连材料，其包含热交联明胶膜，并且通过下式（1）计算的水含量为60-85％：水含量（％）= [（Ws-Wd）/ Ws ]×100（％）（1）在式（1）中，Ws表示浸渍在磷酸盐缓冲盐水溶液中的温度为25℃的抗粘附材料的重量（湿重）1小时， Wd表示使用真空干燥装置完全干燥的防粘附材料的重量（干重）。

公开(公告)号：US20090099268A1

公开(公告)日：2009-04-16

申请号：US11989701

申请日：2006-08-02

申请人： Hitoshi Otani ,  Shojiro Matsuda ,  Tsuguyoshi Taira ,  Noriyuki Morikawa

发明人： Hitoshi Otani ,  Shojiro Matsuda ,  Tsuguyoshi Taira ,  Noriyuki Morikawa

IPC分类号： A61K47/42 ,  C07K14/78 ,  C07K1/00 ,  A61P19/04

CPC分类号： A61K47/42 ,  A61L31/045 ,  C07K1/00 ,  C07K14/78

摘要： An object of the present invention is to provide an anti-adhesion membrane that has no toxicity to a living body, has flexibility allowing itself to fit an affected part as a hydrated gel, is uniformly crosslinked, and is immediately absorbed in a living body after maintaining its shape in the living body for a certain period of time.The present invention provides anti-adhesion material, which comprises a thermally crosslinked gelatin film, and has a water content of 60 to 85% calculated by the following formula (1): water content (%)=[(Ws−Wd)/Ws]×100(%)  (1), in the formula (1), Ws representing a weight (wet weight) of the anti-adhesion material immersed in a phosphate buffered saline solution at a temperature of 25° C. for one hour, and Wd representing a weight (dry weight) of the anti-adhesion material dried completely using a vacuum drying apparatus.

摘要翻译： 本发明的目的是提供一种对生物体无毒性的抗粘附膜，具有使其自身适合作为水合凝胶的患部的柔软性，均匀交联，并且立即被吸收在生物体内 保持其在生命体中的形状一段时间。 本发明提供一种抗粘连材料，其包含热交联的明胶膜，并且通过下式（1）计算的水含量为60-85％：<​​？在线配方说明=“在线式 “end =”lead“？>含水量（％）= [（Ws-Wd）/ Ws] x100（％）（1），<？在线公式描述=”在线公式“end =”tail 式（1）中的“○”表示在25℃的温度下浸渍在磷酸盐缓冲盐水溶液中1小时的抗粘附材料的重量（湿重），Wd表示重量 重量）的防粘附材料使用真空干燥装置完全干燥。