公开(公告)号：US20220135678A1

公开(公告)日：2022-05-05

申请号：US17423894

申请日：2020-01-18

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: C07K16/28 ,  C07K16/30 ,  C12N15/86

Abstract: Described herein are novel compositions and methods to improve the safety and efficacy of adoptive cellular therapies of cancer, infection, allergic, degenerative and immune disorders. The disclosure provides a) novel methods and compositions to reduce the accidental insertion of chimeric receptors into cancer cells; b) novel methods to measure the titer of viral vectors; c) novel compositions to generate antigen masking receptors and methods to use such receptors to protect normal cells from immunotherapeutic agents; d) novel compositions and methods to extend the life-span of allogeneic cells, including allogeneic CAR-T cells; e) novel compositions and methods to ameliorate the side-effects of cellular therapies and f) novel compositions of Synthetic Immune Receptors and Ab-TCRs with mutant TCRα constant chains.

公开(公告)号：US20210290676A1

公开(公告)日：2021-09-23

申请号：US17264788

申请日：2019-07-30

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: A61K35/17 ,  A61K31/496 ,  C07K14/705 ,  C07K14/725

Abstract: The disclosure relates generally to the generation and use of immune effector cells (e.g., T cells, NK cells) engineered to express an immune receptor (e.g., Chimeric Antigen Receptor, Synthetic Immune Receptor, T Cell Receptor etc.) to treat a disease associated with expression of a target antigen.

公开(公告)号：US20190107537A1

公开(公告)日：2019-04-11

申请号：US16089278

申请日：2017-03-31

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/569 ,  G01N33/58

Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.

公开(公告)号：US20240133885A1

公开(公告)日：2024-04-25

申请号：US18449154

申请日：2023-08-13

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/569 ,  C07K16/28 ,  G01N33/50 ,  G01N33/58

CPC classification number: G01N33/56972 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2866 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/2896 ,  G01N33/5023 ,  G01N33/5044 ,  G01N33/56966 ,  G01N33/581 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/43 ,  C07K2319/60 ,  G01N2333/70503 ,  G01N2333/72 ,  G01N2800/52

Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.

公开(公告)号：US20230130925A1

公开(公告)日：2023-04-27

申请号：US17912009

申请日：2021-03-16

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: A61K31/506 ,  A61K31/5025 ,  A61K31/496 ,  C07K16/28 ,  C07K16/24 ,  A61K38/17 ,  A61K31/7076 ,  C07K16/18 ,  A61K45/06 ,  A61K31/513 ,  A61K31/675 ,  A61K31/4965 ,  A61K31/215 ,  A61K31/7056 ,  A61K31/53 ,  A61K31/4706 ,  A61P31/14 ,  A61P11/00 ,  A61P31/16 ,  A61K39/395

Abstract: The present disclosure relates to methods for preventing, ameliorating or treating complications caused by a viral infection (e.g., SARS, SARS-cov2, MERS, Influenza etc.), by administration of an immune response modulating agent (IRMA).

公开(公告)号：US20220252606A1

公开(公告)日：2022-08-11

申请号：US17723284

申请日：2022-04-18

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/58 ,  G01N33/50 ,  C12Q1/44 ,  C12Q1/66

Abstract: Described herein are methods for assessing cytotoxicity of an agent. The methods include providing a target cell that has been engineered to express intracellularly a reporter that is not expressed endogenously by the target cell, exposing the target cell to an agent capable of modulating cytotoxicity and assaying the activity of the reporter, wherein a change in reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell.

公开(公告)号：US20210137977A1

公开(公告)日：2021-05-13

申请号：US15734203

申请日：2019-06-01

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: A61K35/17 ,  C07K14/725 ,  C12N5/0783 ,  A61P35/00

Abstract: The disclosure provides diverse antigen binding domains and platforms for construction of conventional and next generation chimeric antigen receptors for adoptive cellular therapies for cancer, infection, allergic, degenerative and immune disorders, Also provided are approaches for activation and expansion of immune T cells for adoptive cellular therapies for cancer, infection, allergic, degenerative and immune disorders.

公开(公告)号：US20190293656A1

公开(公告)日：2019-09-26

申请号：US16333980

申请日：2017-09-19

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/58 ,  G01N33/50

Abstract: Described herein are methods for assessing cytotoxicity of an agent. The methods include providing a target cell that has been engineered to express intracellularly a reporter that is not expressed endogenously by the target cell, exposing the target cell to an agent capable of modulating cytotoxicity and assaying the activity of the reporter, wherein a change in reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell.

公开(公告)号：US20190209614A1

公开(公告)日：2019-07-11

申请号：US16315153

申请日：2017-07-14

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: A61K35/17 ,  C12N5/0783 ,  A61P35/00 ,  C12N5/0781 ,  A61P31/18 ,  A61P37/06

CPC classification number: A61K35/17 ,  A61P31/18 ,  A61P35/00 ,  A61P37/06 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0647 ,  C12N2503/02 ,  C12N2523/00 ,  C12N2529/10 ,  Y02A50/467

Abstract: Described herein are methods for selecting lymphocytes for adoptive cell therapy based on P-glycoprotein expression and compositions comprising same.

公开(公告)号：US20190112380A1

公开(公告)日：2019-04-18

申请号：US16089106

申请日：2017-03-29

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: C07K16/28 ,  A61K35/17 ,  A61K45/06 ,  A61P35/00

Abstract: Provided herein is a composition comprising, a cell, comprising nucleic acids encoding a chimeric antigen receptor (CAR) and one or more of signaling proteins selected from K13-vFLIP, MC159-vFLIP, cFLIP-L, cFLIP-p22, HTLV1-Tax and HTLV2-Tax, wherein the CAR comprises an a) extracellular antigen specific domain, b) a transmembrane domain and c) an intracellular signaling domain comprising an immunoreceptor tyrosine-based activation motif (ITAM); wherein c) is located at the C-terminus of the chimeric receptor. In some embodiments, the CAR further comprises one or more co-stimulatory domains. Also provided herein are methods for treating diseases using the compositions described herein.