公开(公告)号：US12196759B2

公开(公告)日：2025-01-14

申请号：US17723284

申请日：2022-04-18

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/58 ,  C12Q1/44 ,  C12Q1/66 ,  G01N33/50

Abstract: Described herein are methods for assessing cytotoxicity of an agent. The methods include providing a target cell that has been engineered to express intracellularly a reporter that is not expressed endogenously by the target cell, exposing the target cell to an agent capable of modulating cytotoxicity and assaying the activity of the reporter, wherein a change in reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell.

公开(公告)号：US20240166755A1

公开(公告)日：2024-05-23

申请号：US18525506

申请日：2023-11-30

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: C07K16/28 ,  A61K35/17 ,  A61K45/06 ,  A61K48/00 ,  A61P35/00 ,  C12N15/86

CPC classification number: C07K16/2866 ,  A61K35/17 ,  A61K45/06 ,  A61K48/005 ,  A61P35/00 ,  C07K16/2803 ,  C07K16/2896 ,  C12N15/86 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/33 ,  C12N2740/16043

Abstract: Provided herein is a composition comprising, a cell, comprising nucleic acids encoding a chimeric antigen receptor (CAR) and one or more of signaling proteins selected from K13-vFLIP, MC159-vFLIP, cFLIP-L, cFLIP-p22, HTLV1-Tax and HTLV2-Tax, wherein the CAR comprises an a) extracellular antigen specific domain, b)a transmembrane domain and c) an intracellular signaling domain comprising an immunoreceptor tyrosine-based activation motif (ITAM); wherein c) is located at the C-terminus of the chimeric receptor. In some embodiments, the CAR further comprises one or more co-stimulatory domains. Also provided herein are methods for treating diseases using the compositions described herein.

公开(公告)号：US20240230646A9

公开(公告)日：2024-07-11

申请号：US18449154

申请日：2023-08-14

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/569 ,  C07K16/28 ,  G01N33/50 ,  G01N33/58

CPC classification number: G01N33/56972 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2866 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/2896 ,  G01N33/5023 ,  G01N33/5044 ,  G01N33/56966 ,  G01N33/581 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/43 ,  C07K2319/60 ,  G01N2333/70503 ,  G01N2333/72 ,  G01N2800/52

Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.

公开(公告)号：US11307205B2

公开(公告)日：2022-04-19

申请号：US16333980

申请日：2017-09-19

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/58 ,  G01N33/50 ,  C12Q1/44 ,  C12Q1/66

Abstract: Described herein are methods for assessing cytotoxicity of an agent. The methods include providing a target cell that has been engineered to express intracellularly a reporter that is not expressed endogenously by the target cell, exposing the target cell to an agent capable of modulating cytotoxicity and assaying the activity of the reporter, wherein a change in reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell.

公开(公告)号：US11768203B2

公开(公告)日：2023-09-26

申请号：US16089278

申请日：2017-03-31

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: G01N33/58 ,  G01N33/535 ,  G01N33/569 ,  G01N33/50 ,  C07K16/28

CPC classification number: G01N33/56972 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2866 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/2896 ,  G01N33/5023 ,  G01N33/5044 ,  G01N33/56966 ,  G01N33/581 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/43 ,  C07K2319/60 ,  G01N2333/70503 ,  G01N2333/72 ,  G01N2800/52

Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.

公开(公告)号：US20230212319A1

公开(公告)日：2023-07-06

申请号：US17912423

申请日：2021-03-16

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: C07K16/46 ,  A61P35/00 ,  C07K14/725

CPC classification number: C07K16/468 ,  A61P35/00 ,  C07K14/7051 ,  A61K2039/5156

Abstract: The disclosure relates to compositions and methods of generating synthetic antigen receptors or SAR (e.g., SIR, zSIR, cTCR, ab-TCRs, AABD-TCRs, TFP, TACs etc.) and antibodies (e.g., bispecific antibodies, DARTs etc.) comprising one or more novel antigen binding domains. SARs as described comprise single chain immune receptors (e.g., 1st, 2nd and 3rd generation chimeric antigen receptors, TFPs. Tri-TAC and the like) and multiple chain immune receptors (e.g., SIR, zSIR, cTCR. ab-TCR. AABD-TCR. αβTFP, ydTFP, recombinant TCRs etc.). SARs are able to redirect immune cell specificity and reactivity toward one or more selected targets exploiting the antigen-binding domain properties.

公开(公告)号：US11382931B2

公开(公告)日：2022-07-12

申请号：US16315153

申请日：2017-07-14

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: A61K35/17 ,  C12N5/0783 ,  C12N5/0789 ,  A61P37/06 ,  A61P31/18 ,  A61P35/00 ,  C12N5/0781

Abstract: Described herein are methods for selecting lymphocytes for adoptive cell therapy based on P-glycoprotein expression and compositions comprising same.

公开(公告)号：US20220204582A1

公开(公告)日：2022-06-30

申请号：US16465668

申请日：2017-12-02

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: C07K14/725 ,  A61K35/17 ,  A61K45/06 ,  C07K14/705 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32

Abstract: The disclosure provides synthetic immune receptors (SIRs), nucleic acids encoding the SIRs, methods of making and using the SIRs, in, for example, adoptive cell therapy.

公开(公告)号：US20230140802A1

公开(公告)日：2023-05-04

申请号：US16651907

申请日：2018-09-27

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: C07K14/725 ,  C07K14/705 ,  A61K35/17 ,  C12N5/0783

Abstract: The disclosure provides compositions and method that promote adoptive cellular therapy. The disclosure provides polynucleotides, vectors, systems and cells comprising chimeric antigen receptors (CARs), synthetic immune receptors (SIRs), and the like in combination the specific activators of NFkB activity, thus improving cellular proliferation, expression and reduced apoptosis, which improves cell persistence in adoptive cell therapy.

公开(公告)号：US20220387502A1

公开(公告)日：2022-12-08

申请号：US17861534

申请日：2022-07-11

Applicant: University of Southern California

Inventor： Preet M. Chaudhary

IPC: A61K35/17 ,  C12N5/0783 ,  C12N5/0789 ,  A61P37/06 ,  A61P31/18 ,  A61P35/00 ,  C12N5/0781

Abstract: Described herein are methods for selecting lymphocytes for adoptive cell therapy based on P-glycoprotein expression and compositions comprising same.