公开(公告)号：US20080229456A1

公开(公告)日：2008-09-18

申请号：US11545075

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael H. Luethy ,  Philip W. Miller ,  Larry A. Gilbertson ,  Edwards Allen ,  Sara E. Heisel ,  David K. Kovalic ,  James K. Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael H. Luethy ,  Philip W. Miller ,  Larry A. Gilbertson ,  Edwards Allen ,  Sara E. Heisel ,  David K. Kovalic ,  James K. Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： C12N15/11 ,  A01H5/00 ,  C12N5/10

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

摘要翻译： 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。

公开(公告)号：US20070089187A1

公开(公告)日：2007-04-19

申请号：US11545027

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizzi ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US20070083952A1

公开(公告)日：2007-04-12

申请号：US11545076

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US20070083947A1

公开(公告)日：2007-04-12

申请号：US11544928

申请日：2006-10-10

申请人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

发明人： Shihshieh Huang ,  Thomas Malvar ,  Michael Luethy ,  Philip Miller ,  Larry Gilbertson ,  Edwards Allen ,  Sara Heisel ,  David Kovalic ,  James Roberts ,  Nancy Houmard ,  Alessandra Frizz ,  Yuanji Zhang ,  Liang Guo ,  Linda Lutfiyya

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

摘要： The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

公开(公告)号：US06765117B2

公开(公告)日：2004-07-20

申请号：US10184907

申请日：2002-07-01

申请人： Robert M. Moriarty ,  Raju Penmasta ,  Liang Guo ,  Munagala S. Rao ,  James P. Staszewski

发明人： Robert M. Moriarty ,  Raju Penmasta ,  Liang Guo ,  Munagala S. Rao ,  James P. Staszewski

IPC分类号： C07C3700

CPC分类号： C07C405/00

摘要： An improved method is described for making 9-deoxy-PGF1-type compounds. In contrast to the prior art, the method is stereoselective and requires fewer steps than the known methods for making these compounds. The invention also relates to novel intermediates prepared during the synthesis of the 9-deoxy-PGF1-type compounds.

摘要翻译： 描述了一种用于制备9-脱氧-PGF1型化合物的改进方法。 与现有技术相反，该方法是立体选择性的，并且比制备这些化合物的已知方法需要更少的步骤。 本发明还涉及在合成9-脱氧-PGF1型化合物期间制备的新型中间体。

公开(公告)号：US06441245B1

公开(公告)日：2002-08-27

申请号：US09541521

申请日：2000-04-03

申请人： Robert M. Moriarty ,  Raju Penmasta ,  Liang Guo ,  Munagala S. Rao ,  James P. Staszewski

发明人： Robert M. Moriarty ,  Raju Penmasta ,  Liang Guo ,  Munagala S. Rao ,  James P. Staszewski

IPC分类号： C07C49537

CPC分类号： C07C405/00

摘要： An improved method is described for making 9-deoxy-PGF1-type compounds. In contrast to the prior art, the method is stereoselective and requires fewer steps than the known methods for making these compounds.

公开(公告)号：US06211168B1

公开(公告)日：2001-04-03

申请号：US08483371

申请日：1995-06-07

申请人： Charles W. Bishop ,  Ronald L. Horst ,  Glenville Jones ,  Nicholas J. Koszewski ,  Joyce C. Knutson ,  Robert M. Moriarty ,  Timothy A. Reinhardt ,  Raju Penmasta ,  Stephen Strugnell ,  Liang Guo ,  Sanjay K. Singhal ,  Lei Zhao

发明人： Charles W. Bishop ,  Ronald L. Horst ,  Glenville Jones ,  Nicholas J. Koszewski ,  Joyce C. Knutson ,  Robert M. Moriarty ,  Timothy A. Reinhardt ,  Raju Penmasta ,  Stephen Strugnell ,  Liang Guo ,  Sanjay K. Singhal ,  Lei Zhao

IPC分类号： A61K3159

CPC分类号： A61K31/59 ,  A61K31/592 ,  A61K45/06 ,  C07C401/00 ,  C07J9/00 ,  C07J71/0042 ,  A61K2300/00

摘要： 1&agr;,24(S)-Dihydroxy vitamin D2 which is useful as an active compound of pharmaceutical compositions for the treatment of disorders of calcium metabolism and for various skin disorders. The invention also includes preparation of synthetic 1&agr;,24(S)-dihydroxy vitamin D2 starting from ergosterol which is converted in six steps to 24-hydroxyergosterol. 24-Hydroxyergosterol is irradiated and thermally converted to 24-hydroxy vitamin D2 which is converted in six steps to 1&agr;,24(S)-dihydroxy vitamin D2. The syntheses also produced novel intermediates.

摘要翻译： 1α，24（S） - 二羟基维生素D2，其可用作用于治疗钙代谢紊乱和各种皮肤病症的药物组合物的活性化合物。 本发明还包括从麦角甾醇开始合成的1α，24（S） - 二羟基维生素D2的制备，其从六步转化为24-羟基麦角固醇。 24-羟基麦角甾醇被照射并热转化成24-羟基维生素D2，其被六步转化为1α，24（S） - 二羟基维生素D2。 综合也产生了新的中间体。

公开(公告)号：US11648489B1

公开(公告)日：2023-05-16

申请号：US17808294

申请日：2022-06-22

申请人： Liang Guo

发明人： Liang Guo

IPC分类号： B01D11/02 ,  B01D3/38 ,  B01D3/42

CPC分类号： B01D11/0219 ,  B01D3/38 ,  B01D3/42 ,  B01D11/02

摘要： An herbal distillation device includes a herb chamber with perforated holes for holding herbs and a closed heated boiling chamber with openings at the side for a steam outlet. The herbal distillation device further includes an electric air-cooled condensation chamber which connects to the boiling chamber.

公开(公告)号：US20180268319A1

公开(公告)日：2018-09-20

申请号：US15462675

申请日：2017-03-17

申请人： Liang Guo ,  Hao Yang

发明人： Liang Guo ,  Hao Yang

IPC分类号： G06N99/00 ,  G06N5/04

CPC分类号： G06N20/00 ,  G06N5/04 ,  G06Q30/0204

摘要： A computer system can perform a semi-supervised machine learning processes to cluster a plurality of entities within a population based on their features and associated labels. The computer system can generate visualization data representing the clusters of entities and associated labels for displaying on a user interface. A user can review the clustering of entities and use the user interface to add or modify the labels associated with a particular entity or set of entities. The computer system can use the user's feedback to update the labels and then re-determine the clustering of entities using the semi-supervised machine learning process with the updated labels as input. As such, the computer system can use the user's feedback to improve the accuracy of the machine learning model without requiring a larger amount of labeled input data.

公开(公告)号：US09934078B2

公开(公告)日：2018-04-03

申请号：US13350910

申请日：2012-01-16

申请人： Liang Guo ,  Xu Qin Zhao

发明人： Liang Guo ,  Xu Qin Zhao

IPC分类号： G06F13/00 ,  G06F9/54

CPC分类号： G06F9/543

摘要： A method for collaborative work of applications can include reading a rule for defining a data transmission among collaborative working applications; initiating applications related to the rule; acquiring progress identifiers of the applications; and registering the progress identifiers of the applications.