公开(公告)号：US5433948A

公开(公告)日：1995-07-18

申请号：US945288

申请日：1992-09-10

申请人： Wayne R. Thomas ,  Kaw-Yan Chua

发明人： Wayne R. Thomas ,  Kaw-Yan Chua

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K39/35 ,  A61K39/36 ,  A61K39/395 ,  A61P37/08 ,  C07H21/04 ,  C07K14/435 ,  C07K14/54 ,  C12N15/12 ,  C12P21/02 ,  C12R1/19 ,  C07K19/00

CPC分类号： C07K14/43531 ,  C07K14/5403 ,  A61K38/00 ,  Y10S436/826

摘要： The present invention features isolated DNA encoding allergens of Dermatophagoides (house dust mites) particularly of the species Dermatophagoides farinae and Dermatophagoides pteronyssinus, which are protein allergens or peptides which include at least one epitope of the protein allergen. In particular, the invention provides DNA encoding the major D. farinae allergens, Der f I and Der f II and DNA encoding the major D. pteronyssinus allergens, Der p I and Der p II. The present invention further relates to proteins and peptides encoded by the isolated D. farinae and D. pteronyssinus DNA, including proteins containing sequence polymorphisms. In addition, the proteins or peptides encoded by the isolated DNA, their use a diagnostic and therapeutic reagents and methods of diagnosing and treating sensitivity to house dust mite allergens, are disclosed.

摘要翻译： 本发明的特征在于编码Dermatophagoides（房尘螨）变应原的分离的DNA，特别是Dermatophagoides farinae和Dermatophagoides pteronyssinus，它们是包括蛋白质变应原的至少一个表位的蛋白质过敏原或肽。 特别地，本发明提供编码主要D. farinae过敏原Der f I和Der f II的DNA和编码主要D.pteronyssinus变应原Der P I和Der p II的DNA。 本发明还涉及由分离的D. farinae和D.pteronyssinus DNA编码的蛋白质和肽，包括含有序列多态性的蛋白质。 此外，公开了由分离的DNA编码的蛋白质或肽，它们使用诊断和治疗试剂以及诊断和治疗对尘螨过敏原敏感性的方法。

公开(公告)号：US20120214709A1

公开(公告)日：2012-08-23

申请号：US13341788

申请日：2011-12-30

申请人： Paul M. Watt ,  Wayne R. Thomas

发明人： Paul M. Watt ,  Wayne R. Thomas

IPC分类号： C40B40/08 ,  C40B50/00 ,  C40B50/06 ,  C40B40/06

CPC分类号： C12N15/1086 ,  C07K7/06 ,  C07K7/08 ,  C07K14/001 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1058 ,  C40B30/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/569 ,  G01N33/574 ,  G01N33/6845 ,  Y02A50/57 ,  Y02A50/58

摘要： The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and or immunogenicity, said method comprising the step of: (i) producing a gene fragment expression library derived from defined nucleotide sequence fragments; and (ii) assaying the expression library for at least an amino acid sequence derived from step (i) for a biological activity wherein that activity is different from any activity the amino acid sequence may have in its native environment.

摘要翻译： 本发明提供了鉴定生物活性的调节剂或介体的方法，该活性包括抗原性和/或免疫原性，所述方法包括以下步骤：（i）产生源自确定的核苷酸序列片段的基因片段表达文库; 和（ii）测定所述表达文库至少一种衍生自步骤（i）的氨基酸序列的生物活性，其中所述活性不同于氨基酸序列在其天然环境中可能具有的任何活性。

公开(公告)号：US20110092386A1

公开(公告)日：2011-04-21

申请号：US12908828

申请日：2010-10-20

申请人： Paul M. WATT ,  Wayne R. Thomas

发明人： Paul M. WATT ,  Wayne R. Thomas

IPC分类号： C40B30/00 ,  C40B30/06 ,  C40B30/04

CPC分类号： C12N15/1086 ,  C07K7/06 ,  C07K7/08 ,  C07K14/001 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1058 ,  C40B30/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/569 ,  G01N33/574 ,  G01N33/6845 ,  Y02A50/57 ,  Y02A50/58

摘要： The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and/or immunogenicity, said method comprising the step of: (i) producing a gene fragment expression library derived from defined nucleotide sequence fragments; and (ii) assaying the expression library for at least an amino acid sequence derived from step (i) for a biological activity wherein that activity is different from any activity the amino acid sequence may have in its native environment.

摘要翻译： 本发明提供了鉴定生物活性的调节剂或介体的方法，该活性包括抗原性和/或免疫原性，所述方法包括以下步骤：（i）产生衍生自确定的核苷酸序列片段的基因片段表达文库; 和（ii）测定所述表达文库至少一种衍生自步骤（i）的氨基酸序列的生物活性，其中所述活性不同于氨基酸序列在其天然环境中可能具有的任何活性。

公开(公告)号：US20080139401A1

公开(公告)日：2008-06-12

申请号：US11890714

申请日：2007-08-06

申请人： Paul M. Watt ,  Wayne R. Thomas

发明人： Paul M. Watt ,  Wayne R. Thomas

IPC分类号： C40B30/04

CPC分类号： C12N15/1086 ,  C07K7/06 ,  C07K7/08 ,  C07K14/001 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1058 ,  C40B30/04 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/569 ,  G01N33/574 ,  G01N33/6845 ,  Y02A50/57 ,  Y02A50/58

摘要： The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and or immunogenicity, said method comprising the step of: (i) producing a gene fragment expression library derived from defined nucleotide sequence fragments; and (ii) assaying the expression library for at least an amino acid sequence derived from step (i) for a biological activity wherein that activity is different from any activity the amino acid sequence may have in its native environment.

摘要翻译： 本发明提供了鉴定生物活性的调节剂或介体的方法，该活性包括抗原性和/或免疫原性，所述方法包括以下步骤：（i）产生源自确定的核苷酸序列片段的基因片段表达文库; 和（ii）测定所述表达文库至少一种衍生自步骤（i）的氨基酸序列的生物活性，其中所述活性不同于氨基酸序列在其天然环境中可能具有的任何活性。