公开(公告)号：US20210214687A1

公开(公告)日：2021-07-15

申请号：US17211863

申请日：2021-03-25

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yair REISNER ,  Noga OR-GEVA ,  Rotem GIDRON BUDOVSKY ,  Esther BACHAR-LUSTIG ,  Assaf LASK ,  Sivan KAGAN

IPC: C12N5/0783 ,  A61K35/17 ,  A61K39/00 ,  C12N5/00

Abstract: A method of generating an isolated population of non graft versus host disease (GvHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) providing a population of at least 70% memory T cells; (b) contacting the population of memory T cells with an antigen or antigens so as to allow enrichment of antigen reactive cells; and (c) culturing the cells resulting from step (b) in the presence of cytokines so as to allow proliferation of cells comprising the Tcm phenotype. Cells generated by the method, pharmaceutical compositions and methods of treatment are also disclosed.

公开(公告)号：US20180200300A1

公开(公告)日：2018-07-19

申请号：US15873943

申请日：2018-01-18

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yair REISNER ,  Esther BACHAR-LUSTIG ,  Shlomit REICH-ZELIGER

IPC: A61K35/17 ,  C07K16/28 ,  A61P35/00 ,  A61K35/28 ,  A61K39/395

CPC classification number: A61K35/17 ,  A61K35/28 ,  A61K39/39566 ,  A61P35/00 ,  C07K16/2812 ,  C07K16/2815

Abstract: A method of transplantation is disclosed. The method comprising administering to a subject in need of transplantation of cells in suspension, a therapeutically effective amount of tolerance inducing anti-third party cytotoxic T-lymphocytes (CTLs), wherein the tolerance inducing anti-third party CTLs are generated by directing T-lymphocytes of a donor against a third party antigen or antigens, the tolerance inducing anti-third party CTLs being substantially depleted of T-lymphocytes capable of developing into alloreactive CTLs, and wherein the tolerance inducing anti-third party CTLs do not comprise cells having a central memory T-lymphocyte (Tcm) phenotype, wherein the cells in suspension comprise non-hematopoietic cells or hematopoietic cells which are not stem cells. Methods of treating and kits are also provided.

公开(公告)号：US20180193384A1

公开(公告)日：2018-07-12

申请号：US15825275

申请日：2017-11-29

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yair REISNER ,  Yaki EIDELSTEIN ,  Eran OPHIR ,  Assaf LASK ,  Ran AFIK ,  Noga OR-GEVA ,  Esther BACHAR-LUSTIG

IPC: A61K35/17 ,  A61K39/00 ,  A61K35/26 ,  A61K35/28 ,  A61K35/34 ,  A61K35/36 ,  A61K35/38 ,  A61K35/39 ,  C12N5/0783 ,  A61K35/22 ,  A61K35/42 ,  A61K35/407 ,  A61K35/12

CPC classification number: A61K35/17 ,  A61K35/22 ,  A61K35/26 ,  A61K35/28 ,  A61K35/34 ,  A61K35/36 ,  A61K35/38 ,  A61K35/39 ,  A61K35/407 ,  A61K35/42 ,  A61K39/001 ,  A61K2035/122 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0636 ,  C12N5/0637 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/2321 ,  C12N2502/1121

Abstract: A method of generating an isolated population of cells comprising anti-third party cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance-inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) contacting peripheral blood mononuclear cells (PBMC) with a third party antigen or antigens in the presence of IL-21 so as to allow enrichment of antigen reactive cells; and (b) culturing the cells resulting from step (a) in the presence of IL-21, IL-15 and IL-7 in an antigen free environment so as to allow proliferation of cells comprising the central memory T-lymphocyte (Tcm) phenotype.