利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

244 条记录 (耗时 0.012 秒)

    Process for the production of low-cost soluble high-molecular weight collagen
    11.
    发明授权
    Process for the production of low-cost soluble high-molecular weight collagen 失效
    生产低成本可溶性高分子量胶原蛋白的方法

    公开(公告)号：US5316942A

    公开(公告)日：1994-05-31

    申请号：US78932

    申请日：1993-06-16

    申请人： David J. Fink

    发明人： David J. Fink

    IPC分类号： A61K38/00 ,  C07K14/78 ,  D21H11/14 ,  D21H17/22 ,  D21H21/18 ,  C07K3/12 ,  A61K37/12 ,  C12N9/99 ,  C12P21/00

    CPC分类号： C07K14/78 ,  D21H17/22 ,  A61K38/00 ,  D21H11/14 ,  D21H21/18

    摘要： The invention includes a method that produces a low cost aqueous solution of high molecular weight solubilized collagen by the steps of: (a) providing an aqueous ground slurry of insoluble collagen; (b) adjusting the water or solid content of the wet ground slurry whereby the insoluble collagen is at a concentration that promotes substantially maximum solubilized collagen concentration and molecular weight in a final product; (c) adjusting the pH of the slurry from Step b to obtain activity for a proteolytic enzyme added in Step d; (d) adding the proteolytic enzyme to the pH adjusted slurry and reacting at a temperature, T, and for a time, t, effective for forming high molecualr weight solubilized collagen from the insoluble collagen particles; (e) controlling the reaction conditions for obtaining a high concentration of soluble collagen and a high molecular weight of the solubilized collagen by simultaneously measuring the concentration of solubilized collagen and the molecular weight of the solubilized collagen, whereby the reaction is complete when the molecular weight and the concentration are substantially maximized; and (f) withdrawing the aqueous solution of high molecular weight solubilized collagen as product. Proteolytic enzyme recycle steps are disclosed that can be used to further reduce costs.

    摘要翻译： 本发明包括通过以下步骤产生低成本高分子量溶解胶原的水溶液的方法：（a）提供不溶性胶原的水性研磨浆液; （b）调节湿磨浆液的水分或固体含量，由此不溶性胶原蛋白处于最终产品中促进基本上最大溶解的胶原浓度和分子量的浓度; （c）从步骤b调节浆液的pH以获得在步骤d中加入的蛋白水解酶的活性; （d）将蛋白水解酶加入到pH调节的浆液中，并在从不溶性胶原颗粒形成高分子量溶解胶原的温度T和一段时间t反应; （e）通过同时测量溶解的胶原蛋白的浓度和溶解的胶原的分子量来控制获得高浓度可溶性胶原和高分子量的溶解胶原的反应条件，从而当分子量 并且浓度基本上最大化; 和（f）取出高分子量溶解胶原蛋白水溶液作为产品。 公开了可用于进一步降低成本的蛋白水解酶再循环步骤。