公开(公告)号：US11034992B2

公开(公告)日：2021-06-15

申请号：US16087968

申请日：2017-03-23

申请人： THE GENERAL HOSPITAL CORPORATION

发明人： Sylvie Breton ,  Rachel Liberman ,  Leileata M. Russo

IPC分类号： C12Q1/32 ,  C12Q1/54

摘要： Disclosed herein are methods, assays, compositions and kits for the detection and measurement of UDPglucose in biological samples. The methods, assays, compositions and kits are suitable for use with equipment that is readily available in a clinical laboratory, and permit rapid and reproducible detection and measurement of UDP-glucose at physiologically relevant levels in biological samples.

公开(公告)号：US11029309B2

公开(公告)日：2021-06-08

申请号：US16217550

申请日：2018-12-12

申请人： Roche Diabetes Care, Inc.

发明人： Carina Horn ,  Timo-Jaakko Valta

IPC分类号： G01N33/52 ,  G01N21/77 ,  C12Q1/54 ,  C12Q1/26 ,  C12Q1/32

摘要： The present invention relates to a detector matrix for detecting at least one analyte in a sample, preferably a sample of a body fluid, comprising at least one enzyme active in the presence of said at least one analyte and at least one indicator reagent changing at least one optical property dependent on the activity of said enzyme, wherein said detector matrix further comprises up-converting phosphor particles, preferably UV-emitting up-converting phosphor particles. The invention further relates to a test element and a test device for detecting at least one analyte in a sample comprising the detector matrix of the invention, as well as to a method for the manufacture of a detector matrix, a method for the manufacture of a test element, and to a method for detecting an analyte in a sample, comprising contacting a detector matrix according to the invention with a sample suspected to comprise said analyte.

公开(公告)号：US20210147900A1

公开(公告)日：2021-05-20

申请号：US16951050

申请日：2020-11-18

申请人： F.A.T. Stats LLC

发明人： Tom Meitz ,  Frank Puglisi ,  Joseph Rokowski

IPC分类号： C12Q1/32 ,  G01N21/78 ,  G01N33/52

摘要： Compositions and methods for detecting β-hydroxybutyrate in a biological fluid from a subject in need thereof are disclosed. Also disclosed are compositions and methods of reducing an optical change in a composition for detecting the β-hydroxybutyrate during storage before the composition is exposed to the β-hydroxybutyrate.

公开(公告)号：US10995359B2

公开(公告)日：2021-05-04

申请号：US16903884

申请日：2020-06-17

申请人： NITTO BOSEKI CO., LTD.

发明人： Masayuki Wakabayashi ,  Natsuki Sato ,  Hideki Ishihara

IPC分类号： C12Q1/32 ,  C12Q1/04 ,  C12Q1/48 ,  G01N33/53 ,  A61P35/00 ,  A61K31/366 ,  A61K31/519 ,  G01N33/574

摘要： The present invention addresses the problem of providing a novel method for classifying cancer cells by an analysis method in which measurement of the activity of two types of protein kinase is used. Cancer cells are newly classified and drug sensitivity is predicted on the basis of the ratio of the activity of two types of protein kinase derived from the same sample.

公开(公告)号：US10988738B2

公开(公告)日：2021-04-27

申请号：US16278008

申请日：2019-02-15

申请人： Ikeda Food Research Co., Ltd. ,  PHC Corporation

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

IPC分类号： C12N9/02 ,  C12Q1/32 ,  C12Q1/54 ,  A61K38/00

摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

公开(公告)号：US10851094B2

公开(公告)日：2020-12-01

申请号：US16255535

申请日：2019-01-23

申请人： TERUMO KABUSHIKI KAISHA

发明人： Hiroya Sato

IPC分类号： C07D417/04 ,  C12Q1/26 ,  G01N33/66 ,  G01N33/92 ,  C12Q1/32 ,  C12Q1/60 ,  C12Q1/54 ,  C12Q1/62 ,  C07D513/04

摘要： A 2-substituted benzothiazolyl-3-substituted phenyl-5-substituted sulfonated phenyl-2H-tetrazolium salt represented by the following Formula (1): wherein, R1 can be a hydrogen atom, a hydroxyl group, a methoxy group, and an ethoxy group; R2 can be a nitro group, —OR4, and a carboxyl group; R3 is a hydrogen atom, a methyl group, or an ethyl group, while at least one is a methyl group or an ethyl group; R4 is a methyl group or an ethyl group; m is 1 or 2; n is an integer from 0 to 2; p is 0 or 1; n+p is 1 or greater; q is 1 or 2; when q is 2, the OR3's are disposed adjacently to each other and may form a ring; and X is a hydrogen atom or an alkali metal atom.

公开(公告)号：US20200348255A1

公开(公告)日：2020-11-05

申请号：US16936638

申请日：2020-07-23

申请人： PHC Holdings Corporation

发明人： Yoshifumi TAKAHARA ,  Takahiro NAKAMINAMI ,  Shin IKEDA

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/32

摘要： A reagent layer of a sensor contains as a mediator a quinone compound having a hydrophilic functional group, phenanthrenequinone, and/or a phenanthrenequinone derivative. The quinone compound has a lower redox potential than a conventional mediator, so interfering substances have less effect on detection results with this sensor.

公开(公告)号：US20200332337A1

公开(公告)日：2020-10-22

申请号：US16855534

申请日：2020-04-22

申请人： Qualitic Biotechnology LLC

发明人： Danae Argyropoulou ,  Madhumita Baskaran ,  Pranav Dorbala ,  Allison Julie Wong

IPC分类号： C12Q1/04 ,  C12Q1/32 ,  G01N33/52

摘要： In certain aspects, provided herein are methods and kits for detecting the presence of C. difficile in a biological sample. Also provided herein are methods of selecting of identifying a subject that would benefit from C. difficile treatment.

公开(公告)号：US20200253896A1

公开(公告)日：2020-08-13

申请号：US16796220

申请日：2020-02-20

申请人： The University of Tokyo

发明人： Yuya TERASHIMA ,  Kouji MATSUSHIMA ,  Etsuko TODA ,  Hiroaki TERASAWA ,  Sosuke YOSHINAGA

IPC分类号： A61K31/145 ,  G16B15/00 ,  A61K45/06 ,  C12Q1/32 ,  G01N33/68

摘要： An agent according to the present invention comprises as an effective component any of (1) disulfiram, diethyldithiocarbamate, or a metal complex of diethyldithiocarbamate; (2) a pharmaceutically acceptable salt of (1); or (3) a solvate of (1) or (2), and is used for inhibition of interaction between CR2B or CCR5 and FROUNT protein, inhibition of macrophages, control of cells constituting a cancer microenvironment or inflammatory microenvironment, or enhancement of anticancer activity of an anticancer drug. It is also possible to provide a compound with a reduced side effect and an increased pharmacological effect by identifying a disulfiram derivative having a lower aldehyde dehydrogenase-inhibiting activity and a higher FROUNT-inhibiting activity among derivatives prepared by structural modification of disulfiram.

公开(公告)号：US10731199B2

公开(公告)日：2020-08-04

申请号：US14359177

申请日：2012-11-20

申请人： ADVANCED LIQUID LOGIC, INC.

发明人： Carrie A. Graham ,  Allen E. Eckhardt

IPC分类号： C12Q1/32

摘要： Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.