公开(公告)号：US20100233725A1

公开(公告)日：2010-09-16

申请号：US12403299

申请日：2009-03-12

Applicant: Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

Inventor： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC: G01N33/53

CPC classification number: G01N33/78 ,  G01N33/582 ,  G01N2333/59

Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

Abstract translation: 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US20070122850A1

公开(公告)日：2007-05-31

申请号：US11619244

申请日：2007-01-03

Applicant: Zhu Teng ,  Jeffrey Moore ,  Alan Craig ,  Gary Hickey ,  Carsten Schelp ,  Tie Wei

Inventor： Zhu Teng ,  Jeffrey Moore ,  Alan Craig ,  Gary Hickey ,  Carsten Schelp ,  Tie Wei

IPC: G01N33/53 ,  C12M1/34

CPC classification number: G01N33/548 ,  G01N33/54393

Abstract: Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.

Abstract translation: 公开了用于在用于测定样品中分析物的结合测定中的非特异性结合的方法和组合物，其中用于进行结合测定的试剂之一包含含有多糖的固体支持物。 该方法包括在用于进行结合测定的测定培养基中包含可与多糖连接的蛋白质的可溶性化合物。 还公开了用于确定疑似含有分析物的样品中分析物的存在和/或量的方法和组合物。 所述方法包括作为试剂的固体支持物，其包含多糖和包含与多糖连接的蛋白质的可溶性化合物。

公开(公告)号：US20050118642A1

公开(公告)日：2005-06-02

申请号：US10725070

申请日：2003-12-01

Applicant: Carsten Schelp ,  Michael Trier ,  Hrair Kirakossian

Inventor： Carsten Schelp ,  Michael Trier ,  Hrair Kirakossian

IPC: G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01N33/53

CPC classification number: G01N33/582 ,  G01N33/536 ,  G01N33/542 ,  G01N33/54306

Abstract: Methods for quantitatively or qualitatively detecting an analyte in a sample and suitable reagents for this purpose, in particular to a homogeneous binding test, with one analyte-specific binding partner R1 possessing more than one specific binding site for a specific binding partner X, which is associated with a component of a signal-generating system, and a second analyte-specific binding partner R2 possessing more than one specific binding site for a specific binding partner Y, which is likewise associated with a component of a signal-generating system.

Abstract translation: 用于定量或定性检测样品中的分析物的方法以及用于此目的的合适的试剂，特别是与均一结合试验，一种具有特定结合配偶体X的多于一个特异性结合位点的分析物特异性结合配偶体R1，其为 与信号产生系统的组件相关联，以及第二分析物特异性结合配偶体R2，其具有特定结合配偶体Y的多于一个特异性结合位点，其同样与信号发生系统的组分相关联。

公开(公告)号：US20050112586A1

公开(公告)日：2005-05-26

申请号：US10720909

申请日：2003-11-24

Applicant: Roland Janzen ,  Carsten Schelp

Inventor： Roland Janzen ,  Carsten Schelp

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/543

CPC classification number: G01N33/54333

Abstract: The invention relates to methods and compositions for removing a dissociated species from a fluid medium solution during and after it has detached from a solid-phase immersed in said medium, thereby allowing the concentration of free species to remain close to zero, and for improving the signal to noise ratio in assays. This is achieved by employing a substrate, such as a scavenging solid-phase, having an attached binding partner or partners (“scavenger”) for the specifically binding species and which is present during storage. This substrate may also contain regions for binding signal generating components attached to the solid-phase. This substrate binds any free species bleeding off the solid phase, increasing the reliability and sensitivity of assays. A subset of the substrates in the invention additionally forms cross-linked networks of solid-phase particles that further increase the sensitivity of assays.

Abstract translation: 本发明涉及用于在液体介质溶液从浸入所述介质中的固相脱离时从流体介质溶液中除去离解物质的方法和组合物，从而允许游离物质的浓度保持接近零，并且为了改善 测定中的信噪比。 这通过使用具有附着的结合配偶体或伴侣（“清道夫”）的底物，例如清除固相，并且在储存期间存在。 该衬底还可以包含用于结合连接到固相的信号产生部件的区域。 该底物结合任何自由物质脱离固相，增加了测定的可靠性和灵敏度。 本发明的底物的一个子集另外形成进一步提高测定灵敏度的固相颗粒的交联网络。