公开(公告)号：US09347947B2

公开(公告)日：2016-05-24

申请号：US12403299

申请日：2009-03-12

申请人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

发明人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC分类号： G01N21/76 ,  G01N33/58

CPC分类号： G01N33/78 ,  G01N33/582 ,  G01N2333/59

摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

摘要翻译： 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US20100233725A1

公开(公告)日：2010-09-16

申请号：US12403299

申请日：2009-03-12

申请人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

发明人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC分类号： G01N33/53

CPC分类号： G01N33/78 ,  G01N33/582 ,  G01N2333/59

摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

摘要翻译： 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US20050118727A1

公开(公告)日：2005-06-02

申请号：US10725166

申请日：2003-12-01

申请人： Carsten Schelp ,  Pratap Singh ,  Michael Trier ,  Harald Ackermann ,  Susan Swann ,  Lee Hall

发明人： Carsten Schelp ,  Pratap Singh ,  Michael Trier ,  Harald Ackermann ,  Susan Swann ,  Lee Hall

IPC分类号： G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/58 ,  G01N33/543

CPC分类号： G01N33/582 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542

摘要： Methods for quantitatively or qualitatively detecting an analyte in a sample and suitable reagents for this purpose, in particular to a carrier molecule to which one or more analyte-specific binding partner(s) is/are bonded and which possesses additional binding sites for a specific binding partner X or Y which is associated with a component of a signal-generating system, and to the use of such a conjugate, in particular in homogeneous binding tests.

摘要翻译： 用于定量或定性检测样品中的分析物和用于此目的的合适试剂的方法，特别是与一种或多种分析物 - 特异性结合配偶体结合并且具有特定的分析物特异性结合配偶体的另外的结合位点的载体分子 与信号发生系统的组分相关联的结合配偶体X或Y，以及这种缀合物的使用，特别是在均匀结合测试中。

公开(公告)号：US20100248218A1

公开(公告)日：2010-09-30

申请号：US11443301

申请日：2006-05-31

申请人： Carsten Schelp ,  Pratap Singh ,  Michael Trier ,  Harald Ackermann ,  Susan C. Swann ,  Lee Hall

发明人： Carsten Schelp ,  Pratap Singh ,  Michael Trier ,  Harald Ackermann ,  Susan C. Swann ,  Lee Hall

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12N9/96 ,  G01N33/543 ,  G01N33/545 ,  C07K14/76 ,  C07K16/00 ,  C07H21/00 ,  C08B37/02 ,  C07H1/00 ,  C07J17/00 ,  C07D495/04 ,  C07D311/82 ,  C07C205/00

CPC分类号： G01N33/582 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542

摘要： The invention relates to methods for the quantitative and qualitative detection of an analyte in an assay as well as appropriate reagents therefor, especially a carrier molecule to which one or several analyte-specific binding partners are bound and which is provided with additional binding points for a specific binding partner X or Y that is associated with a component of a signal-forming system. Also disclosed is the use of such a conjugate particularly in homogeneous binding tests.

摘要翻译： 本发明涉及用于在测定中定量和定性检测分析物的方法以及其适合的试剂，特别是与一种或几种分析物特异性结合配偶体相结合的载体分子，并且其具有额外的结合点用于 与信号形成系统的组件相关联的特异性结合配偶体X或Y。 还公开了这种缀合物的使用，特别是在均匀结合试验中。

公开(公告)号：US07220842B2

公开(公告)日：2007-05-22

申请号：US10818115

申请日：2004-04-05

申请人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

发明人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

IPC分类号： C07K17/02 ,  C07D491/02 ,  G01N33/535

CPC分类号： C07K16/44 ,  G01N33/9486

摘要： Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

摘要翻译： 公开了针对丁丙诺啡（BUP）和丁丙诺啡（norBUP）的半抗原，免疫原和免疫测定法的方法，组合物和试剂盒。 该方法包括在介质中提供（i）怀疑含有丁丙诺啡（BUP）或降丁非诺（norBUP））的样品和（ii）针对丁丙诺啡（BUP）或去诺贝芬（norBUP）的免疫原产生的抗体。 检查介质是否存在包含丁丙诺啡（BUP）或降丁非诺（norBUP）的标记半抗原的复合物，其中诸如复合物的存在表明样品中存在化合物。

公开(公告)号：US20050221405A1

公开(公告)日：2005-10-06

申请号：US10818115

申请日：2004-04-05

申请人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

发明人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

IPC分类号： G01N33/53 ,  G01N33/94

CPC分类号： C07K16/44 ,  G01N33/9486

摘要： Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

摘要翻译： 公开了针对丁丙诺啡（BUP）和丁丙诺啡（norBUP）的半抗原，免疫原和免疫测定法的方法，组合物和试剂盒。 该方法包括在介质中提供（i）怀疑含有丁丙诺啡（BUP）或降丁非诺（norBUP））的样品和（ii）针对丁丙诺啡（BUP）或去诺贝芬（norBUP）的免疫原产生的抗体。 检查介质是否存在包含丁丙诺啡（BUP）或降丁非诺（norBUP）的标记半抗原的复合物，其中诸如复合物的存在表明样品中存在化合物。

公开(公告)号：US20140356885A1

公开(公告)日：2014-12-04

申请号：US13905459

申请日：2013-05-30

申请人： Rafael Bartz ,  Pratap Singh ,  Liping Geng ,  Gladys Privon

发明人： Rafael Bartz ,  Pratap Singh ,  Liping Geng ,  Gladys Privon

IPC分类号： G01N33/53

CPC分类号： G01N33/54333

摘要： Methods and reagents are disclosed for preparing a support for reaction of the support with an assay molecule. In the method the support is treated with a detergent at a concentration of about 0.01% to about 5% (by weight) at a temperature of about 4° C. to about 50° C. for a period of about 1 hour to about 24 hours and subsequently the support is washed. The support is contacted with the assay molecule under conditions for covalently binding the assay molecule to the support to form a conjugate of the support and the assay molecule.

摘要翻译： 公开了用于制备载体与测定分子反应的载体的方法和试剂。 在该方法中，在约4℃​​至约50℃的温度下，以约0.01％至约5％（重量）的浓度的洗涤剂处理载体约1小时至约24 小时，随后洗涤支持。 在将分析分子共价结合到载体以形成载体和测定分子的缀合物的条件下，将支持物与测定分子接触。

公开(公告)号：US07863427B2

公开(公告)日：2011-01-04

申请号：US11734661

申请日：2007-04-12

申请人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

发明人： Yi-Feng Zheng ,  Pratap Singh ,  Dorota Bolle ,  Hshiou-Ting Liu

IPC分类号： C07K17/02 ,  C07D491/02

CPC分类号： C07K16/44 ,  G01N33/9486

摘要： Methods, compositions and kits are disclosed directed at haptens, immunogens and immnoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

摘要翻译： 公开了针对丁丙诺啡（BUP）和丁丙诺啡（norBUP）的半抗原，免疫原和免疫测定法的方法，组合物和试剂盒。 该方法包括在介质中提供（i）怀疑含有丁丙诺啡（BUP）或降丁非诺（norBUP））的样品和（ii）针对丁丙诺啡（BUP）或去诺贝芬（norBUP）的免疫原产生的抗体。 检查介质是否存在包含丁丙诺啡（BUP）或降丁非诺（norBUP）的标记半抗原的复合物，其中诸如复合物的存在表明样品中存在化合物。

公开(公告)号：US20140212987A1

公开(公告)日：2014-07-31

申请号：US13753909

申请日：2013-01-30

申请人： Pratap Singh ,  Roland Janzen ,  Jie Li ,  Nina Zolotarjova

发明人： Pratap Singh ,  Roland Janzen ,  Jie Li ,  Nina Zolotarjova

IPC分类号： G01N33/82

CPC分类号： G01N33/82 ,  G01N33/5306 ,  G01N33/6854

摘要： Methods of enhancing signal ratio between calibrators in an assay for an analyte include conducting an assay for the analyte with zero concentration of analyte in a first calibrator to determine a first signal level. The reagents employed in the assay comprise an antibody reagent comprising an antibody for the analyte wherein a hinge region of the antibody is conjugated to a moiety. The assay for the analyte is also conducted with a second concentration of analyte in a second calibrator to determine a second signal level wherein the second analyte concentration is greater than zero and wherein the reagents employed in the assay comprise the antibody reagent. A ratio of the first signal level to the second signal level is determined and evaluated.

摘要翻译： 在分析物测定中增强校准器之间的信号比率的方法包括在第一校准器中对零分析物进行分析物测定以确定第一信号水平。 在测定中使用的试剂包含抗体试剂，其包含用于分析物的抗体，其中抗体的铰链区域与部分缀合。 分析物的测定还用第二校准器中的第二浓度的分析物进行，以确定第二分析物浓度大于零的第二信号水平，并且其中测定中使用的试剂包含抗体试剂。 确定并评估第一信号电平与第二信号电平的比率。

公开(公告)号：US20110032136A1

公开(公告)日：2011-02-10

申请号：US12512824

申请日：2009-07-30

申请人： Pratap Singh ,  Chandrajit Debnath

发明人： Pratap Singh ,  Chandrajit Debnath

IPC分类号： H03M1/12 ,  H03K5/22

CPC分类号： H03K3/356139 ,  H03K3/013

摘要： The present disclosure relates to reduction in the effect of kickback in comparators by means of charge injection implemented by means of voltage controlled switches with attributes similar to those of an input differential pair. The voltage controlled switches produce charge to neutralize the charge loss during latching of inputs in the comparator.

摘要翻译： 本公开涉及通过借助于具有与输入差分对的属性类似的电压控制开关实现的电荷注入来减少比较器中反冲的影响。 电压控制开关产生电荷以中和比较器中的输入锁存期间的电荷损失。