公开(公告)号：US20050139537A1

公开(公告)日：2005-06-30

申请号：US11037356

申请日：2005-01-19

Applicant: Chung-Jen Hou ,  Peter Konstantin ,  Yujing Yang

Inventor： Chung-Jen Hou ,  Peter Konstantin ,  Yujing Yang

IPC: B01D15/36 ,  B01D61/00 ,  B01D61/14 ,  B01D67/00 ,  B01D71/08 ,  B01D71/40 ,  B01J20/28 ,  B01J20/32 ,  B01J39/20 ,  B01J39/26 ,  B01J47/12 ,  C07K1/34 ,  C12M1/34 ,  C02F1/44

CPC classification number: B01D67/0088 ,  B01D15/362 ,  B01D61/00 ,  B01D61/007 ,  B01D61/14 ,  B01D67/0009 ,  B01D71/08 ,  B01D71/40 ,  B01D2323/12 ,  B01D2323/30 ,  B01D2325/14 ,  B01D2325/36 ,  B01J20/261 ,  B01J20/262 ,  B01J20/264 ,  B01J20/267 ,  B01J20/28035 ,  B01J20/2804 ,  B01J20/28078 ,  B01J20/2808 ,  B01J20/286 ,  B01J20/3208 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3274 ,  B01J20/3282 ,  B01J39/20 ,  B01J39/26 ,  B01J47/12 ,  B01J2220/44 ,  B01J2220/54 ,  C07K1/34 ,  Y10T436/143333

Abstract: The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.

Abstract translation: 本发明在某些实施方案中提供了一种带负电荷的微多孔膜，其包含多孔基底和具有固定负电荷的交联涂层。 交联涂层可以例如由含有阴离子基团的不饱和单体，N-（羟甲基） - 和/或N-（烷氧基甲基） - 丙烯酰胺，亲水性不饱和单体和引发剂的聚合组合物制备。 在一些实施方案中，本发明进一步提供了一种带负电荷的微多孔膜，其包含多孔基材和由聚合组合物制备的交联涂层，所述聚合组合物包含具有阴离子基团的不饱和单体，N-（羟甲基） - 或N-（烷氧基甲基） - 丙烯酰胺，多糖和引发剂。 本发明的膜适用于离子交换色谱法，例如分离和纯化带正电荷的物质如蛋白质。

公开(公告)号：US4250141A

公开(公告)日：1981-02-10

申请号：US969847

申请日：1978-12-15

Applicant: Hans-Dieter Lehmann ,  Peter Konstantin

Inventor： Hans-Dieter Lehmann ,  Peter Konstantin

IPC: A61M1/36 ,  B01D15/00 ,  B01J47/02 ,  A61M1/03 ,  B01D15/04

CPC classification number: A61M1/3679 ,  B01D15/00

Abstract: A column for removing poisons and/or other undesired substances from a liquid mixture. The column has a mixture of inactive filler particles and active granules positioned therein, with said filler particles being substantially larger than said active granules, (which are selected from adsorbing material and ion-exchanging material). The filler particles are in fixed contact relationship with each other and the active granules completely fill the spaces between the filler particles.

Abstract translation: 用于从液体混合物中除去毒物和/或其他不需要物质的柱。 该柱具有位于其中的惰性填料颗粒和活性颗粒的混合物，所述填料颗粒基本上大于所述活性颗粒（其选自吸附材料和离子交换材料）。 填料颗粒彼此处于固定的接触关系，活性颗粒完全填充填料颗粒之间的空间。