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公开(公告)号：US20100047849A1
公开(公告)日：2010-02-25
申请号：US12607905
申请日：2009-10-28
Applicant: Michael P. Caulfield , Darren A. Carns , Richard E. Reitz
Inventor： Michael P. Caulfield , Darren A. Carns , Richard E. Reitz
IPC: C12Q1/02
CPC classification number: G01N33/743 , Y10T436/108331 , Y10T436/200833
Abstract: Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d5 testosterone.
Abstract translation: 提供了用于确定测试样品中睾丸酮的存在或量的方法，其包括电离样品中存在的全部或部分睾酮以产生在质谱仪中可检测的一种或多种睾酮离子。 存在于样品中的全部或一部分睾酮被离子化以产生一种或多种睾丸酮离子，其可被分离并碎裂以产生前体离子。 可以在样品中提供可单独检测的内部睾酮标准品。 在优选的实施方案中，参考文献是2,2,4,6,6-d5睾酮。
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公开(公告)号：US20100032558A1
公开(公告)日：2010-02-11
申请号：US12189092
申请日：2008-08-08
Applicant: Cory E. Bystrom , Richard E. Reitz , Nigel J. Clarke
Inventor： Cory E. Bystrom , Richard E. Reitz , Nigel J. Clarke
IPC: B01D59/44
CPC classification number: G01N33/6848 , G01N2333/96416
Abstract: Provided are methods for measuring renin activity in a plasma sample using mass spectrometry. The methods generally involve ionizing purified angiotensin 1 from the sample and detecting the amount of angiotensin 1 ions generated. The amount of detected angiotensin 1 ions are then related to the amount of angiotensin 1 generated in the sample, which in turn is related to renin activity in the sample.
Abstract translation: 提供了使用质谱法测量血浆样品中的肾素活性的方法。 该方法通常涉及从样品离子化纯化的血管紧张素1并检测产生的血管紧张素1离子的量。 然后检测到的血管紧张素1离子的量与样品中产生的血管紧张素1的量相关，其又与样品中的肾素活性相关。
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公开(公告)号：US20090148951A1
公开(公告)日：2009-06-11
申请号：US12001076
申请日：2007-12-06
Applicant: Yanni Zhang , Nigel J. Clarke , Richard E. Reitz
Inventor： Yanni Zhang , Nigel J. Clarke , Richard E. Reitz
CPC classification number: G01N33/6848 , G01N33/68 , G01N33/6893 , G01N33/78 , G01N2800/52 , G01N2800/7028 , Y10T436/24
Abstract: Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.
Abstract translation: 提供了使用各种纯化步骤随后进行质谱法测定样品中甲状腺球蛋白量的方法。 所述方法通常涉及在测试样品中纯化甲状腺球蛋白，消化甲状腺球蛋白以形成肽T129，纯化肽T129，电离肽T129，检测产生的肽T129离子的量，并将肽T129离子的量与原始存在的甲状腺球蛋白量相关联 在样品中。
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24.发明申请公开(公告)号：US20090137056A1
公开(公告)日：2009-05-28
申请号：US11946765
申请日：2007-11-28
Applicant: Brett Holmquist , Nigel J. Clarke , Ann Caston-Balderrama , Richard E. Reitz
Inventor： Brett Holmquist , Nigel J. Clarke , Ann Caston-Balderrama , Richard E. Reitz
IPC: G01N33/00
CPC classification number: G01N27/62 , G01N33/82 , Y10T436/10 , Y10T436/20 , Y10T436/203332 , Y10T436/24
Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin in D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.
Abstract translation: 提供了使用质谱法检测样品中二羟基维生素D代谢物的存在或量的方法。 方法通常包括在样品中离子化二羟基维生素D代谢物，并检测离子的量以确定样品中维生素D代谢物的存在或量。 在某些优选的实施方案中，所述方法包括在质谱法之前免疫促进二羟维生素D代谢物。 还提供了在单个测定中检测两种或更多种二羟维生素D代谢物的存在或量的方法。
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公开(公告)号：US07348137B2
公开(公告)日：2008-03-25
申请号：US11247409
申请日：2005-10-11
Applicant: Michael P. Caulfield , Darren A Carns , Richard E Reitz
Inventor： Michael P. Caulfield , Darren A Carns , Richard E Reitz
CPC classification number: G01N33/743 , Y10T436/108331 , Y10T436/200833
Abstract: Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d5 testosterone.
Abstract translation: 提供了用于确定测试样品中睾丸酮的存在或量的方法，其包括电离样品中存在的全部或部分睾酮以产生在质谱仪中可检测的一种或多种睾酮离子。 存在于样品中的全部或一部分睾酮被离子化以产生一种或多种睾丸酮离子，其可被分离并碎裂以产生前体离子。 可以在样品中提供可单独检测的内部睾酮标准品。 在优选的实施方案中，参考文献是2,2,4,6,6-d 5睾酮。
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Patent Agency Ranking
公开(公告)号：US08709818B2
公开(公告)日：2014-04-29
申请号：US13589404
申请日：2012-08-20
Applicant: Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
Inventor： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
CPC classification number: G01N33/92 , C07K1/14 , G01N15/0266 , G06F19/00 , G06F19/10 , G16H50/30 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
Abstract translation: 本发明提供了利用差示带电粒子迁移率分析方法从生物样品制备脂蛋白的方法，包括HDL，LDL，Lp（a），IDL和VLDL，用于诊断目的。 还提供了通过差示带电粒子迁移率分析脂蛋白的大小分布的方法，该脂蛋白通过本发明的方法制备。 还提供了评估脂质相关健康风险，心血管疾病，心血管疾病风险以及对治疗性干预的反应性的方法，该方法利用通过本发明方法测定的脂蛋白大小分布。
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公开(公告)号：US08455259B2
公开(公告)日：2013-06-04
申请号：US13198620
申请日：2011-08-04
Applicant: Yanni Zhang , Nigel J. Clarke , Richard E. Reitz
Inventor： Yanni Zhang , Nigel J. Clarke , Richard E. Reitz
CPC classification number: G01N33/6848 , G01N33/68 , G01N33/6893 , G01N33/78 , G01N2800/52 , G01N2800/7028 , Y10T436/24
Abstract: Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.
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公开(公告)号：US08409862B2
公开(公告)日：2013-04-02
申请号：US12946785
申请日：2010-11-15
Applicant: Michael P. Caulfield , Darren A Carns , Richard E Reitz
Inventor： Michael P. Caulfield , Darren A Carns , Richard E Reitz
IPC: G01N33/92
CPC classification number: G01N33/743 , Y10T436/108331 , Y10T436/200833
Abstract: Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d5 testosterone.
Abstract translation: 提供了用于确定测试样品中睾丸酮的存在或量的方法，其包括使样品中存在的全部或部分睾酮电离以产生在质谱仪中可检测的一种或多种睾酮离子。 存在于样品中的全部或一部分睾酮被离子化以产生一种或多种睾丸酮离子，其可被分离并碎裂以产生前体离子。 可以在样品中提供可单独检测的内部睾酮标准品。 在优选的实施方案中，参考文献是2,2,4,6,6-d5睾酮。
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公开(公告)号：US08329392B2
公开(公告)日：2012-12-11
申请号：US13118180
申请日：2011-05-27
Applicant: Michael P. Caulfield , Darren A Carns , Richard E Reitz
Inventor： Michael P. Caulfield , Darren A Carns , Richard E Reitz
CPC classification number: G01N33/743 , Y10T436/108331 , Y10T436/200833
Abstract: Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d5 testosterone.
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30.发明授权公开(公告)号：US08173442B2
公开(公告)日：2012-05-08
申请号：US13117997
申请日：2011-05-27
Applicant: Brett Holmquist , Nigel J. Clarke , Anne Caston-Balderrama , Richard E. Reitz
Inventor： Brett Holmquist , Nigel J. Clarke , Anne Caston-Balderrama , Richard E. Reitz
IPC: G01N24/00
CPC classification number: G01N27/62 , G01N33/82 , Y10T436/10 , Y10T436/20 , Y10T436/203332 , Y10T436/24
Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.
Abstract translation: 提供了使用质谱法检测样品中二羟基维生素D代谢物的存在或量的方法。 方法通常包括在样品中离子化二羟基维生素D代谢物，并检测离子的量以确定样品中维生素D代谢物的存在或量。 在某些优选的实施方案中，所述方法包括在质谱法之前免疫促进二羟维生素D代谢物。 还提供了在单个测定中检测两种或更多种二羟维生素D代谢物的存在或量的方法。
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