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公开(公告)号：US08808643B1
公开(公告)日：2014-08-19
申请号：US13228391
申请日：2011-09-08
IPC分类号： B01L3/02
CPC分类号： B01L3/502715 , B01L3/502738 , B01L2300/0864 , B01L2400/0622
摘要： Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.
摘要翻译： 本文提供了流动平台和样品制备和分析方法。 流体学平台能够使用诸如聚合酶链反应测定和环介导等温扩增测定之类的扩增测定法从血液样品中分析DNA。 流体学平台也可用于其他类型的测定和分析。 在一些实施例中，密封管中的样品可以直接插入。 无需用户干预即可执行以下隔离，检测和分析。 所公开的平台还可以包括具有磁性致动器，加热器和空气干燥机构的样品制备系统，以及用于在反应之后和样品之间的萃取，洗涤，洗脱，测定组装，测定检测和清洁的流体操纵过程。
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公开(公告)号：US20120315706A1
公开(公告)日：2012-12-13
申请号：US13589404
申请日：2012-08-20
申请人： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
发明人： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
CPC分类号： G01N33/92 , C07K1/14 , G01N15/0266 , G06F19/00 , G06F19/10 , G16H50/30 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
摘要翻译： 本发明提供了利用差示带电粒子迁移率分析方法从生物样品制备脂蛋白的方法，包括HDL，LDL，Lp（a），IDL和VLDL，用于诊断目的。 还提供了通过差示带电粒子迁移率分析脂蛋白的大小分布的方法，该脂蛋白通过本发明的方法制备。 还提供了评估脂质相关健康风险，心血管疾病，心血管疾病风险以及对治疗性干预的反应性的方法，该方法利用通过本发明方法测定的脂蛋白大小分布。
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公开(公告)号：US08247235B2
公开(公告)日：2012-08-21
申请号：US11760672
申请日：2007-06-08
申请人： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
发明人： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
CPC分类号： G01N33/92 , C07K1/14 , G01N15/0266 , G06F19/00 , G06F19/10 , G16H50/30 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
摘要翻译： 本发明提供了利用差示带电粒子迁移率分析方法从生物样品制备脂蛋白的方法，包括HDL，LDL，Lp（a），IDL和VLDL，用于诊断目的。 还提供了通过差示带电粒子迁移率分析脂蛋白的大小分布的方法，该脂蛋白通过本发明的方法制备。 还提供了评估脂质相关健康风险，心血管疾病，心血管疾病风险以及对治疗性干预的反应性的方法，该方法利用通过本发明方法测定的脂蛋白大小分布。
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4.发明授权Method of assessing a lipid-related health risk based on ion mobility analysis of lipoproteins 有权基于脂蛋白离子迁移率分析评估与脂质相关的健康风险的方法公开(公告)号：US07851224B2
公开(公告)日：2010-12-14
申请号：US12775225
申请日：2010-05-06
CPC分类号： G01N15/0266 , G01N33/92 , G01N2800/52 , Y10T436/104165 , Y10T436/24
摘要： A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.
摘要翻译： 描述了用于分析非共价结合的附聚生物颗粒的医学诊断方法和仪器系统。 该方法和系统包括：一种制备生物颗粒的方法; 电喷发生器; α粒子辐射源; 差分迁移率分析仪; 粒子计数器 数据采集和分析手段。 通过脂蛋白部分密度的快速定量分析，医疗装置可用于评估人类疾病，如心脏病风险和高脂血症。 最初，描述了纯化程序以将初始血液样品减少到仪器的分析输入。 来自分析样品的测量尺寸与密度相关，导致脂蛋白密度谱。 然后可以将脂蛋白密度分布用于表征心脏和其他与脂质有关的健康风险。
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公开(公告)号：US5880466A
公开(公告)日：1999-03-09
申请号：US869282
申请日：1997-06-02
申请人： W. Henry Benner
发明人： W. Henry Benner
CPC分类号： H01J49/4245 , H01J49/027 , H01J49/04
摘要： The design and operation of a new type of charged-particle trap provides simultaneous measurements of mass, charge, and velocity of large electrospray ions. The trap consists of a detector tube mounted between two sets of center-bored trapping plates. Voltages applied to the trapping plates define symmetrically-opposing potential valleys which guide axially-injected ions to cycle back and forth through the charge-detection tube. A low noise charge-sensitive amplifier, connected to the tube, reproduces the image charge of individual ions as they pass through the detector tube. Ion mass is calculated from measurement of ion charge and velocity following each passage through the detector.
摘要翻译： 新型带电粒子陷阱的设计和操作可以同时测量大型电喷雾离子的质量，电荷和速度。 陷阱由安装在两组中心钻孔捕集板之间的探测器管组成。 施加到捕获板的电压限定对称的相对的电位谷，其引导轴向注入的离子来循环通过电荷检测管。 连接到管的低噪声电荷敏感放大器在通过检测器管时再现各离子的图像电荷。 每次通过检测器后，离子电荷和速度的测量计算出离子质量。
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公开(公告)号：US20210278369A1
公开(公告)日：2021-09-09
申请号：US17259081
申请日：2019-07-10
申请人： W. Henry Benner , Michael J. Bogan , Ben Aguilar
发明人： W. Henry Benner , Michael J. Bogan , Ben Aguilar
IPC分类号： G01N27/622
摘要： Apparatuses and methods are described for determining properties of ions travelling through a gas under the influence of an electric field. The apparatuses and methods can be understood to provide measurements of the electrical mobility of ions as useful for determining the electrical mobility constant Ko of electrosprayed substances, such as proteins. The apparatuses and methods relate to the scientific discipline of ion mobility spectrometry. Modules connected to ion mobility spectrometers provide stress to substances for the purpose of investigating, for example, the thermal stability of proteins. One form of the technology includes a tubular spectrometer body having an electrically conductive inner wall; a rod positioned along the longitudinal center of the body and electrodes positioned on, but electrically isolated from, the inner wall, where the ratio of the radius of the tubular spectrometer body to the ratio of the radius of the rod is at least 20.
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公开(公告)号：US09372135B1
公开(公告)日：2016-06-21
申请号：US13228370
申请日：2011-09-08
申请人： W. Henry Benner , John M. Dzenitis
发明人： W. Henry Benner , John M. Dzenitis
CPC分类号： G01N1/405 , B01L3/5027 , B01L3/50273 , B01L3/502761 , B01L2200/0631 , B01L2200/0647 , B01L2200/0668 , B01L2400/0475 , B01L2400/0478 , B01L2400/0605 , G01N1/286 , G01N1/40 , G01N1/44 , G01N35/0098
摘要： Provided herein are fluidics platforms and related methods for performing integrated sample collection and solid-phase extraction of a target component of the sample all in one tube. The fluidics platform comprises a pump, particles for solid-phase extraction and a particle-holding means. The method comprises contacting the sample with one or more reagents in a pump, coupling a particle-holding means to the pump and expelling the waste out of the pump while the particle-holding means retains the particles inside the pump. The fluidics platform and methods herein described allow solid-phase extraction without pipetting and centrifugation.
摘要翻译： 本文提供了流体平台和相关方法，用于在一个管中进行样品收集和样品的目标组分的固相萃取。 流体平台包括泵，用于固相萃取的颗粒和颗粒保持装置。 该方法包括使样品与泵中的一种或多种试剂接触，将颗粒保持装置耦合到泵并将废物排出泵，同时颗粒保持装置将颗粒保持在泵内。 本文所述的流体学平台和方法允许固相萃取而不需要移液和离心。
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公开(公告)号：US08709818B2
公开(公告)日：2014-04-29
申请号：US13589404
申请日：2012-08-20
申请人： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
发明人： Michael P. Caulfield , Richard E. Reitz , Shuguang Li , Gloria Kwangja Lee , Ronald Krauss , Patricia J. Blanche , W. Henry Benner , Earl Cornell
CPC分类号： G01N33/92 , C07K1/14 , G01N15/0266 , G06F19/00 , G06F19/10 , G16H50/30 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.
摘要翻译： 本发明提供了利用差示带电粒子迁移率分析方法从生物样品制备脂蛋白的方法，包括HDL，LDL，Lp（a），IDL和VLDL，用于诊断目的。 还提供了通过差示带电粒子迁移率分析脂蛋白的大小分布的方法，该脂蛋白通过本发明的方法制备。 还提供了评估脂质相关健康风险，心血管疾病，心血管疾病风险以及对治疗性干预的反应性的方法，该方法利用通过本发明方法测定的脂蛋白大小分布。
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公开(公告)号：US20110089037A1
公开(公告)日：2011-04-21
申请号：US12966739
申请日：2010-12-13
IPC分类号： G01N27/447 , C07K14/775 , C07K14/005 , C07K16/00
CPC分类号： G01N15/0266 , G01N33/92 , G01N2800/52 , Y10T436/104165 , Y10T436/24
摘要： A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.
摘要翻译： 描述了用于分析非共价结合的附聚生物颗粒的医学诊断方法和仪器系统。 该方法和系统包括：一种制备生物颗粒的方法; 电喷发生器; α粒子辐射源; 差分迁移率分析仪; 粒子计数器 数据采集和分析手段。 通过脂蛋白部分密度的快速定量分析，医疗装置可用于评估人类疾病，如心脏病风险和高脂血症。 最初，描述了纯化程序以将初始血液样品减少到仪器的分析输入。 来自分析样品的测量尺寸与密度相关，导致脂蛋白密度谱。 然后可以将脂蛋白密度分布用于表征心脏和其他与脂质有关的健康风险。
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公开(公告)号：US07259018B2
公开(公告)日：2007-08-21
申请号：US10293610
申请日：2002-11-12
CPC分类号： G01N15/0266 , G01N33/92 , G01N2800/52 , Y10T436/104165 , Y10T436/24
摘要： A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.
摘要翻译： 描述了用于分析非共价结合的附聚生物颗粒的医学诊断方法和仪器系统。 该方法和系统包括：一种制备生物颗粒的方法; 电喷发生器; α粒子辐射源; 差分迁移率分析仪; 粒子计数器 数据采集和分析手段。 通过脂蛋白部分密度的快速定量分析，医疗装置可用于评估人类疾病，如心脏病风险和高脂血症。 最初，描述了纯化程序以将初始血液样品减少到仪器的分析输入。 来自分析样品的测量尺寸与密度相关，导致脂蛋白密度谱。 然后可以将脂蛋白密度分布用于表征心脏和其他与脂质有关的健康风险。
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