公开(公告)号：US09006535B2

公开(公告)日：2015-04-14

申请号：US11942134

申请日：2007-11-19

Applicant: Su-May Yu ,  Tuan-Hua David Ho

Inventor： Su-May Yu ,  Tuan-Hua David Ho

IPC: A01H1/00 ,  A01H5/00 ,  C12N15/82 ,  A23L1/10

CPC classification number: C12N15/8237 ,  A23L7/10 ,  C12N15/8271 ,  C12N15/8273

Abstract: In a transgenic plant, a stress-responsive promoter containing CE3 and ABRE2 from HVA1 ABRC3 and CE1 from HVA22 ABRC1, directs low background expression of a gene under normal growth conditions, but is highly inducible by an abiotic stress, such as abscisic acid, dehydration, salt or cold. Compared with the wild type plant, the transgenic plant that expresses a stress-resistant gene under the control of the stress-responsive promoter is more resistant and/or tolerant to abiotic stresses and exhibits similar or higher growth rate and strength under regular or normal environmental conditions.

Abstract translation: 在转基因植物中，含有来自HVA1ABRC3的CE3和ABRE2的应激响应启动子和来自HVA22ABRC1的CE1在正常生长条件下指导基因的低背景表达，但是由非生物胁迫如脱落酸，脱水高度诱导 ，盐或冷。 与野生型植物相比，在胁迫响应启动子控制下表达抗胁迫基因的转基因植物对非生物胁迫具有更强的抗性和/或耐受性，并且在常规或正常环境下表现出相似或更高的生长速率和强度 条件。

公开(公告)号：US08426677B2

公开(公告)日：2013-04-23

申请号：US12604808

申请日：2009-10-23

Applicant: Su-May Yu ,  Shuen-Fang Lo ,  Liang-Jwu Chen ,  Tuan-Hua David Ho

Inventor： Su-May Yu ,  Shuen-Fang Lo ,  Liang-Jwu Chen ,  Tuan-Hua David Ho

IPC: A01H5/00 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/05

CPC classification number: C12N15/8297 ,  C12N9/0071 ,  C12N15/8273

Abstract: Novel gibberellin 2-oxidase (GA2ox) genes were identified. Differential expression of GA2ox genes correlated with flower development, seed germination, tiller growth and other developmental processes. In addition, the early and increased growth of tiller and adventitious root and altered root architecture caused by overexpression of GA2oxs further suggest the pleiotropic role of GA2oxs in controlling growth and architecture in plants such as rice. GA2ox5, GA2ox6 and GA2ox9 were three genes encoding class C20 GA2oxs in rice. Mutants or transgenic rice overexpressing class C20 GA2oxs exhibited a broad range of mutant phenotypes, including semi-dwarfism, increased root system and higher tiller numbers that may favor grain yield. Mutations in the conserved domain III were found to affect the physiological activity of class C20 GA2oxs. Methods are described for controlling plant growth and architecture by controlling gene expression of gibberellin 2-oxidase in the plant.

Abstract translation: 鉴定了新型赤霉素2-氧化酶（GA2ox）基因。 GA2ox基因的差异表达与花的发育，种子发芽，分蘖生长等发育过程相关。 此外，由于GA2ox过表达引起的分蘖和不定根的早期增长和根系的改变进一步表明GA2oxs在控制水稻等植物生长和结构方面具有多方位的作用。 GA2ox5，GA2ox6和GA2ox9是水稻中编码C20 GA2oxs的三个基因。 突变体或转基因水稻超过C20类型的GA2oxs表现出广泛的突变表型，包括半侏儒症，增加的根系和较高的分蘖数，可能有利于谷物产量。 发现保守结构域III中的突变会影响C20类GA2氧化酶的生理活性。 描述了通过控制植物中赤霉素2-氧化酶的基因表达来控制植物生长和结构的方法。

公开(公告)号：US08129140B2

公开(公告)日：2012-03-06

申请号：US13089620

申请日：2011-04-19

Applicant: Tuan-Hua David Ho ,  Su-May Yu ,  I Son Ng ,  Chii-Gong Tong

Inventor： Tuan-Hua David Ho ,  Su-May Yu ,  I Son Ng ,  Chii-Gong Tong

IPC: C12P1/00 ,  C12N9/42

CPC classification number: C12N9/2437 ,  C07H3/02 ,  C07H3/04 ,  C12N15/8246 ,  C12Y302/01004

Abstract: A novel thermophilic endo-glucanase, nucleic acid encoding the endo-glucase, and uses thereof in converting ligocellulosic material to fermentable sugars.

Abstract translation: 一种新型嗜热内切葡聚糖酶，编码内切葡聚糖的核酸，及其用于将纤维素纤维素材料转化成可发酵糖的用途。

公开(公告)号：US20120036597A1

公开(公告)日：2012-02-09

申请号：US13197187

申请日：2011-08-03

Applicant: Su-May Yu ,  Chin-Fen Su

Inventor： Su-May Yu ,  Chin-Fen Su

IPC: A01H5/00 ,  C40B30/04 ,  C12Q1/68 ,  C12N15/82

CPC classification number: C12N15/8273

Abstract: Disclosed are chill- or cold-tolerant plants and methods of making the plants. Also disclosed are methods for identifying a plant that is tolerant to chill.

Abstract translation: 公开了冷或冷耐受植物和制造植物的方法。 还公开了用于鉴定耐寒的植物的方法。

公开(公告)号：US07348471B2

公开(公告)日：2008-03-25

申请号：US10819371

申请日：2004-04-05

Applicant: Su-May Yu ,  Chwan-Yang Hong

Inventor： Su-May Yu ,  Chwan-Yang Hong

IPC: A01H1/00 ,  C07H21/04 ,  C12N15/00

CPC classification number: C12N15/8234 ,  C12N15/8257

Abstract: A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

Abstract translation: 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

公开(公告)号：US07291768B2

公开(公告)日：2007-11-06

申请号：US10630636

申请日：2003-07-30

Applicant: Su-May Yu

Inventor： Su-May Yu

IPC: C12N15/29 ,  C12N15/82 ,  C12N15/87 ,  A01H5/00

CPC classification number: C12N15/8217 ,  C07K14/415

Abstract: A pure polypeptide containing an amino acid sequence at least 70% identical to SEQ ID NO:7, 8, or 9. The polypeptide regulates expression of a gene in a cell. Also disclosed is an isolated nucleic acid characterized in that it hybridizes under stringent conditions to SEQ ID NO:1, 2, or 3, or a complementary sequence thereof. Also within the scope of the invention are a transformed cell or a transgenic plant containing such a nucleic acid and a method of identifying a compound that modulates the activity of the polypeptide.

Abstract translation: 含有与SEQ ID NO：7,8或9至少70％相同的氨基酸序列的纯多肽。该多肽调节基因在细胞中的表达。 还公开了分离的核酸，其特征在于其在严格条件下与SEQ ID NO：1,2或3或其互补序列杂交。 也在本发明的范围内的是含有这种核酸的转化细胞或转基因植物以及鉴定调节多肽活性的化合物的方法。

公开(公告)号：US07094958B2

公开(公告)日：2006-08-22

申请号：US10060515

申请日：2002-01-30

Applicant: Su-May Yu

Inventor： Su-May Yu

IPC: C12N15/11 ,  C12N15/63 ,  C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8234 ,  C12N15/8216 ,  C12N15/8238

Abstract: A recombinant nucleic acid containing a transcriptional enhancer from a rice αAmy8 gene, a promoter operably linked to the enhancer, and a coding sequence operably linked to the promoter and encoding a transcript. Also disclosed is a stably transformed plant cell or a transgenic plant containing a recombinant nucleic acid described above, or a similar recombinant nucleic acid with a plurality of a rice αAmy3 enhancer.

公开(公告)号：US06376750B1

公开(公告)日：2002-04-23

申请号：US09575574

申请日：2000-05-22

Applicant: Su-May Yu ,  Yu-Chan Chao

Inventor： Su-May Yu ,  Yu-Chan Chao

IPC: A01H500

CPC classification number: C12N15/8234 ,  C07K14/415

Abstract: The invention relates to the promoter of a rice glycine-rich RNA binding protein gene and its use for expression of heterologous proteins in plant tissues, including tissues of the plant embryo and seedling.

Abstract translation: 本发明涉及水稻富含甘氨酸的RNA结合蛋白基因的启动子及其在植物组织中表达异源蛋白质的用途，包括植物胚胎和幼苗的组织。

公开(公告)号：US06288302B1

公开(公告)日：2001-09-11

申请号：US09072917

申请日：1998-05-04

Applicant: Su-May Yu ,  Li-Fei Liu ,  Ming-Tsair Chan

Inventor： Su-May Yu ,  Li-Fei Liu ,  Ming-Tsair Chan

IPC: C12N1529

CPC classification number: C12N15/8238 ,  C12N15/8222 ,  C12N15/8234

Abstract: Disclosed is a method for producing a transgenic angiosperm plant, particularly a monocot, comprising the step of transforming an immature embryo of an angiosperm plant with a DNA fragment expressible in said angiosperm embryo, said DNA fragment comprising a promoter region derived from an &agr;-amylase gene of a plant, and an exogenous gene encoding a desired gene product, said promoter region being inducible under a sugar-depleted or sugar-free condition to promote the expression of said gene, whereby at least a part of the resultant transgenic angiosperm plant has the ability to express said gene product. Transgenic angiosperm plants and transgenic angiosperm plant seeds are also disclosed. The transgenic angiosperm plant seeds may have application in the industrial production of alcohol, beer, glucose and the like.

Abstract translation: 公开了一种用于生产转基因被子植物，特别是单子叶植物的方法，包括用被子植物胚胎中可表达的DNA片段转化被子植物植物的未成熟胚的步骤，所述DNA片段包含源于α-淀粉酶的启动子区 植物的基因和编码所需基因产物的外源基因，所述启动子区域在无糖或无糖条件下可诱导以促进所述基因的表达，由此所得转基因被子植物的至少一部分具有 表达所述基因产物的能力。 还披露了转基因被子植物和转基因被子植物种子。 转基因被子植物种子可用于工业生产酒精，啤酒，葡萄糖等。

公开(公告)号：US08114443B2

公开(公告)日：2012-02-14

申请号：US12055502

申请日：2008-03-26

Applicant: Su-May Yu ,  Ya-Fang Hong

Inventor： Su-May Yu ,  Ya-Fang Hong

IPC: A61K38/00 ,  A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC classification number: C12N15/8223 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8257

Abstract: Disclosed are a transgenic plant and a transformed plant cell containing a heterologous nucleic acid that includes a sweet potato sporamin SPO promoter operably linked to a sequence encoding a polypeptide. Also disclosed are uses of the plant or cell.

Abstract translation: 公开了一种转基因植物和含有异源核酸的转化植物细胞，其包含与编码多肽的序列可操作地连接的甘薯孢子淀粉SPO启动子。 还公开了植物或细胞的用途。