公开(公告)号：US20180135115A1

公开(公告)日：2018-05-17

申请号：US15351312

申请日：2016-11-14

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Nicholas M. Sampas ,  Nazumi Alice Yama-Dang ,  Paige Anderson

IPC: C12Q1/68

CPC classification number: C12Q1/6832 ,  C12Q1/6837 ,  C12Q2565/514

Abstract: Provided herein, among other things, are compositions for improving signal-to-noise in hybridization to oligonucleotide arrays. In some embodiments, the composition may comprise a population of indexing oligonucleotides of formula X-Y, where the sequences of region X hybridize to an array and the sequences of region Y hybridize to distinct sites within a genomic region, transcript, set of transcripts, or complement of the same. Use of these oligonucleotides increases signal, reduces background and smoothens array hybridization analyses. Methods and kits that employ the compositions are also provided.

公开(公告)号：US09771575B2

公开(公告)日：2017-09-26

申请号：US14745174

申请日：2015-06-19

Applicant: Agilent Technologies, Inc.

Inventor： Alexander Belyaev ,  Nicholas M. Sampas ,  Alicia Scheffer-Wong

IPC: C12Q1/68 ,  C12N15/10 ,  C12N9/12 ,  C07H21/04

CPC classification number: C12N15/1065 ,  C12Q2521/327 ,  C12Q2563/179

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.

公开(公告)号：US20150361422A1

公开(公告)日：2015-12-17

申请号：US14550713

申请日：2014-11-21

Applicant: Agilent Technologies, Inc.

Inventor： Jeffrey R. Sampson ,  Nicholas M. Sampas ,  Joel Myerson ,  Paige Anderson ,  Bo Curry

IPC: C12N15/10 ,  B01J19/00

Abstract: Provided herein, among other things, is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. Other embodiments are also provided.

Abstract translation: 本文提供了一种方法，其包括：（a）获得多组寡核苷酸的混合物，其中每组中的寡核苷酸各自包含末端分子序列可以组装以产生合成子; 和（b）将寡核苷酸混合物与阵列杂交，从而将不同组的寡核苷酸彼此空间分离。 还提供了其他实施例。

公开(公告)号：US20150290613A1

公开(公告)日：2015-10-15

申请号：US14684028

申请日：2015-04-10

Applicant: Agilent Technologies, Inc.

Inventor： Nicholas M. Sampas ,  Richard P. Tella

IPC: B01J19/00

Abstract: Provided is an emulsion comprising: (a) droplets that contain a single polymeric compound or a pre-defined mixture thereof, and (b) an immiscible liquid, wherein: (i) each of the droplets comprises multiple molecules of the compound(s) contained therein; and the droplets do not contain monomeric precursors for the polymeric compound. A method for making the emulsion is also provided.

Abstract translation: 提供一种乳液，其包含：（a）含有单一聚合物或其预定混合物的液滴，和（b）不混溶液体，其中：（i）每个液滴包含多个化合物分子， 包含在其中 并且液滴不含有用于聚合物的单体前体。 还提供了制备乳液的方法。