利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

25 条记录 (耗时 0.025 秒)

    Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry
    24.
    发明申请
    Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry 审中-公开
    通过激光烧蚀电喷雾离子化质谱法检测代谢状态的方法

    公开(公告)号：US20100285446A1

    公开(公告)日：2010-11-11

    申请号：US12774533

    申请日：2010-05-05

    申请人： Akos Vertes ,  Fatah Kashanchi ,  Prabhakar Sripadi ,  Kylene Kehn-Hall

    发明人： Akos Vertes ,  Fatah Kashanchi ,  Prabhakar Sripadi ,  Kylene Kehn-Hall

    IPC分类号： C12Q1/70 ,  H01J49/26 ,  C12Q1/68 ,  C12Q1/02

    CPC分类号： H01J49/165 ,  G01N33/5091 ,  G01N33/56911 ,  G01N33/56983 ,  G01N33/56988 ,  G01N33/6851 ,  G01N33/6893 ,  G01N2333/15 ,  G01N2333/16 ,  G01N2800/04 ,  H01J49/0463

    摘要： According to certain embodiments, a method of mass spectrometry may generally comprise subjecting a sample comprising at least one indicator to laser ablation electrospray ionization mass spectrometry; determining a relative intensity of the indicator; and comparing the relative intensity of the indicator to a standard indicator intensity. Subjecting a sample to laser ablation electrospray ionization mass spectrometry may comprise ablating the sample with an infrared laser under ambient conditions to form an ablation plume; intercepting the ablation plume by an electrospray plume; and detecting the indicator by mass spectrometry. The method of mass spectrometry may comprise classifying the sample as belonging to or not belonging to the standard indicator intensity. A sample not belonging to the standard indicator intensity may indicate that the sample is predicted to comprise a disease state.

    摘要翻译： 根据某些实施方案，质谱方法通常可以包括使包含至少一个指示剂的样品经受激光消融电喷雾离子化质谱; 确定指标的相对强度; 并将指标的相对强度与标准指标强度进行比较。 将样品照射到激光消融电喷雾电离质谱法可以包括在环境条件下用红外激光烧蚀样品以形成消融羽流; 通过电喷雾羽流拦截消融羽流; 并通过质谱法检测指示剂。 质谱法可以包括将样品归类或不属于标准指示剂强度。 不属于标准指标强度的样品可能表明样品被预测为包含疾病状态。

    Protein microscope
    25.
    发明授权
    Protein microscope 有权
    蛋白质显微镜

    公开(公告)号：US07735146B2

    公开(公告)日：2010-06-08

    申请号：US11795687

    申请日：2006-01-26

    申请人： Akos Vertes ,  Mark E. Reeves ,  Fatah Kashanchi

    发明人： Akos Vertes ,  Mark E. Reeves ,  Fatah Kashanchi

    IPC分类号： H01J49/40

    CPC分类号： G01Q30/02 ,  G01Q60/22 ,  H01J49/0004

    摘要： A system and method for analyzing and imaging a sample containing molecules of interest combines modified MALDI mass spectrometer and SNOM devices and techniques, and includes: (A) an atmospheric-pressure or near-atmospheric-pressure ionization region; (B) a sample holder for holding the sample; (C) a laser for illuminating said sample; (D) a mass spectrometer having at least one evacuated vacuum chamber; (E) an atmospheric pressure interface connecting said ionization region and said mass spectrometer; (F) a scanning near-field optical microscopy instrument comprising a near-field probe for scanning the sample; a vacuum capillary nozzle for sucking in particles which are desorbed by said laser, the nozzle being connected to an inlet orifice of said atmospheric pressure interface; a scanner platform connected to the sample holder, the platform being movable to a distance within a near-field distance of the probe; and a controller for maintaining distance information about a current distance between said probe and said sample; (G) a recording device for recording topography and mass spectrum measurements made during scanning of the sample with the near-field probe; (H) a plotting device for plotting said topography and mass spectrum measurements as separate x-y mappings; and (I) an imaging device for providing images of the x-y mappings.

    摘要翻译： 用于分析和成像含有感兴趣的分子的样品的系统和方法结合了改进的MALDI质谱仪和SNOM装置和技术，并且包括：（A）大气压或近大气压电离区域; （B）用于保持样品的样品架; （C）用于照亮所述样品的激光器; （D）具有至少一个真空室的质谱仪; （E）连接所述电离区和所述质谱仪的大气压界面; （F）扫描近场光学显微镜仪器，其包括用于扫描样品的近场探针; 用于吸入由所述激光器解吸的颗粒的真空毛细管喷嘴，所述喷嘴连接到所述大气压界面的入口孔; 连接到样品架的扫描仪平台，所述平台可移动到所述探针的近场距离内的距离; 以及控制器，用于保持关于所述探针和所述样本之间的当前距离的距离信息; （G）用于记录在用近场探针扫描样品期间进行的地形和质谱测量的记录装置; （H）用于将所述地形和质谱测量绘制为单独的x-y映射的绘图装置; 和（I）用于提供x-y映射的图像的成像装置。