公开(公告)号：US07449552B2

公开(公告)日：2008-11-11

申请号：US11404297

申请日：2006-04-14

申请人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

发明人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

IPC分类号： C07K14/325

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.

公开(公告)号：US07772370B2

公开(公告)日：2010-08-10

申请号：US11833598

申请日：2007-08-03

申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Natalia Ellanskaya, legal representative ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： C07K14/00 ,  C07K14/415

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US20090260106A1

公开(公告)日：2009-10-15

申请号：US11833535

申请日：2007-08-03

申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellenskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellenskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07H21/00 ,  C12N5/04

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

公开(公告)号：US07414174B2

公开(公告)日：2008-08-19

申请号：US11693803

申请日：2007-03-30

申请人： Andre R. Abad ,  Billy F. McCutchen ,  James F. Wong ,  Cao Guo Yu

发明人： Andre R. Abad ,  Billy F. McCutchen ,  James F. Wong ,  Cao Guo Yu

IPC分类号： C12N15/09 ,  C12N15/82 ,  C12N15/31 ,  A01H5/10

CPC分类号： C12N15/8286 ,  C12N9/20 ,  Y02A40/162

摘要： Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

公开(公告)号：US07923599B2

公开(公告)日：2011-04-12

申请号：US11833535

申请日：2007-08-03

申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Natalia Ellanskaya, legal representative ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Elleskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  C07H21/04

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US20100303762A1

公开(公告)日：2010-12-02

申请号：US12795770

申请日：2010-06-08

申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： A01N63/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  A01P3/00

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US20090264351A1

公开(公告)日：2009-10-22

申请号：US11833598

申请日：2007-08-03

申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： A01N37/18 ,  C07K14/00 ,  A01P3/00

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US08148510B2

公开(公告)日：2012-04-03

申请号：US12795770

申请日：2010-06-08

申请人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Natalia Ellanskaya, legal representative ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

发明人： Daniel J. Altier ,  Glen Dahlbacka ,  Irina Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  Eric Schepers ,  Carl R. Simmons ,  Tamas Torok ,  Nasser Yalpani

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US07423197B2

公开(公告)日：2008-09-09

申请号：US11693807

申请日：2007-03-30

申请人： Andre R. Abad ,  Billy F. McCutchen ,  James F. Wong ,  Cao Guo Yu

发明人： Andre R. Abad ,  Billy F. McCutchen ,  James F. Wong ,  Cao Guo Yu

IPC分类号： C12N15/09 ,  C12N15/82 ,  C12N15/31 ,  A01H5/00

CPC分类号： C12N15/8286 ,  C12N9/20 ,  Y02A40/162

摘要： Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

摘要翻译： 提供了通过将非植物脂肪酶引入植物来产生和增强植物中抗虫性的方法。 提供具有增强的抗虫性的植物和植物种子。 还提供了可用于本发明实践的编码杀虫脂肪酶和杀虫脂肪酶基因产物的DNA序列。 本发明的组合物和方法可用于各种用于防治害虫的农业系统，包括传播昆虫抗性作物谱系，并将这些杀虫脂肪酶靶向特别容易受到侵染的植物器官的表达，如根和叶 。

公开(公告)号：US07491869B2

公开(公告)日：2009-02-17

申请号：US11693823

申请日：2007-03-30

申请人： Andre R. Abad ,  Billy F. McCutchen ,  James F. Wong ,  Cao Guo Yu

发明人： Andre R. Abad ,  Billy F. McCutchen ,  James F. Wong ,  Cao Guo Yu

IPC分类号： A01H5/00 ,  C12N15/09 ,  C12N15/82

CPC分类号： C12N15/8286 ,  C12N9/20 ,  Y02A40/162

摘要： Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

摘要翻译： 提供了通过将非植物脂肪酶引入植物来产生和增强植物中抗虫性的方法。 提供具有增强的抗虫性的植物和植物种子。 还提供了可用于本发明实践的编码杀虫脂肪酶和杀虫脂肪酶基因产物的DNA序列。 本发明的组合物和方法可用于各种用于防治害虫的农业系统，包括传播昆虫抗性作物谱系，并将这些杀虫脂肪酶靶向特别容易受到侵染的植物器官的表达，如根和叶 。