摘要:
Multimodal optical imaging is disclosed. Possible devices include a filter assembly that is capable of rotary action during real time image acquisition. Systems are disclosed that include such filter assemblies, which are detachable or integrated in the systems. Various techniques for capturing image targets using multiple light sources such as white light and fluorescent light are also disclosed.
摘要:
Provided herein are devices, systems, and methods for multimodal optical imaging. The devices include a filter assembly that is capable of rotary action during real time image acquisition. The systems include such filter assemblies, which are detachable or integrated in the systems. The methods include techniques for capturing image targets using multiple light sources such as white light and fluorescent light.
摘要:
In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value.
摘要:
An iris imaging system is provided. The iris imaging system includes at least one light source configured to illuminate an iris at a spectrum of light. The iris imaging system also includes at least one image capturing device including at least one of an indium-gallium-arsenide, lead sulphide, and lead selenide based detector and configured to capture an image of the iris via light reflected from the iris at the spectrum. The device is further configured to provide a signal representing one or more features of the iris in response to the light reflected from the iris.
摘要:
A method of autofocusing includes capturing first, second and third images of a sample, at respective first, second and third sample distances and respective first, second and third lateral positions determined with respect to an objective; determining a quantitative characteristic for the first, second and third images; determining a primary sample distance based upon at least the quantitative characteristics for the first, second, and third images; and capturing a primary image of the sample at the primary sample distance and at a primary lateral position that is offset from the first, second and third lateral positions.
摘要:
A system, in one embodiment, includes an optical coherence tomography (OCT) imaging system having a light source configured to emit light. The OCT imaging system further includes a beam splitter configured to receive the light from the light source, split the light into a first light portion directed along a sample arm comprising a sample and a second light portion directed along a reference arm comprising a reference mirror, receive a first reflected light portion from the sample arm and a second reflected light portion from the reference arm, combine the first and second reflected light portions to obtain an interference signal at a detector. Further, the OCT imaging system includes a controller having logic configured to perform an auto-focusing process to determine the optimal position for a lens in the sample arm in order to bring the sample into focus.
摘要:
A method of delivering exogenous molecules, comprising: providing a plurality of cells having a cell membrane; adding a plurality of exogenous molecules to the cells; exposing the cells to a defocused infrared (IR) light to permeabilize the cell membrane of the cells; and delivering the exogenous molecules to the cells through the permeablized cell membrane, wherein an intensity of the IR light at the optical focus is at least greater than or equal to an order of 104 W/cm2.
摘要翻译:一种递送外源性分子的方法,包括:提供多个具有细胞膜的细胞; 向细胞中加入多种外源性分子; 将细胞暴露于散焦的红外(IR)光以使细胞的细胞膜透化; 并且通过透过细胞膜将外源性分子递送至细胞,其中所述光学焦点处的IR光的强度至少大于或等于104W / cm 2。
摘要:
A method of fluorescence imaging is provided. The method provides for simultaneously acquiring image data at a plurality of phases and a plurality of frequencies from a region of interest, identifying at least one desired signal and at least one background signal in the acquired image data associated with the region of interest, constructing a digital filter based upon the at least one desired signal and the at least one background signal, wherein the digital filter is configured to enhance image contrast and applying the digital filter to the acquired image data associated with the region of interest to enhance image contrast in the acquired image data. Systems and computer programs that afford functionality of the type defined by this method are also provided.
摘要:
Disclosed are systems for intraoperative nerve imaging using compact high collection power imaging devices for fluorescence and white light imaging of myelin basic protein (MBP) nerve imaging agents during open and minimally invasive surgery.
摘要:
Disclosed are systems for intraoperative nerve imaging using compact high collection power imaging devices for fluorescence and white light imaging of myelin basic protein (MBP) nerve imaging agents during open and minimally invasive surgery.