公开(公告)号：US10280455B2

公开(公告)日：2019-05-07

申请号：US15393103

申请日：2016-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas Heredia ,  Dianna Maar

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6816 ,  C12Q1/6858

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.

公开(公告)号：US20170166957A1

公开(公告)日：2017-06-15

申请号：US15411482

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

公开(公告)号：US09556475B2

公开(公告)日：2017-01-31

申请号：US14457863

申请日：2014-08-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。

公开(公告)号：US20150148250A1

公开(公告)日：2015-05-28

申请号：US14457863

申请日：2014-08-12

Applicant: Bio-Rad laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。