公开(公告)号：US10280455B2

公开(公告)日：2019-05-07

申请号：US15393103

申请日：2016-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas Heredia ,  Dianna Maar

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6816 ,  C12Q1/6858

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.

公开(公告)号：US20170191127A1

公开(公告)日：2017-07-06

申请号：US15394396

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Shawn Hodges ,  Nicholas Heredia

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1068 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/686 ,  C40B50/06 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2563/159

Abstract: Methods of preparing a target gene-enriched library are provided. In one aspect, the method comprises partitioning polynucleotide fragments into a plurality of partitions, wherein each partition further comprises a plurality of primer pairs for amplifying a target gene and wherein the primers comprise a portion of an adapter sequence; amplifying a target gene sequence to generate an amplicon comprising the target gene sequence flanked on either end by a portion of an adapter sequence; purifying the amplicon; and amplifying the amplicon using primers comprising full-length adapter sequences.

公开(公告)号：US10730030B2

公开(公告)日：2020-08-04

申请号：US15400756

申请日：2017-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00 ,  C12Q1/6806

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

公开(公告)号：US20170191124A1

公开(公告)日：2017-07-06

申请号：US15393103

申请日：2016-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas Heredia ,  Dianna Maar

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2565/1015

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.

公开(公告)号：US11759761B2

公开(公告)日：2023-09-19

申请号：US17381096

申请日：2021-07-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q2525/131 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2523/319 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

公开(公告)号：US10920270B2

公开(公告)日：2021-02-16

申请号：US16361704

申请日：2019-03-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas Heredia ,  Dianna Maar

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.

公开(公告)号：US11898201B2

公开(公告)日：2024-02-13

申请号：US17148440

申请日：2021-01-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nicholas Heredia ,  Dianna Maar

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6858 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853

CPC classification number: C12Q1/6865 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/6858 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q1/6816 ,  C12Q2565/1015

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.

公开(公告)号：US11097247B2

公开(公告)日：2021-08-24

申请号：US16911961

申请日：2020-06-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00 ,  C12Q1/6806

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

公开(公告)号：US20170232417A1

公开(公告)日：2017-08-17

申请号：US15400756

申请日：2017-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.