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    Compositions and kits for molecular counting
    23.
    发明授权
    Compositions and kits for molecular counting 有权

    公开(公告)号：US10941396B2

    公开(公告)日：2021-03-09

    申请号：US14381488

    申请日：2013-02-27

    Applicant: Cellular Research, Inc.

    Inventor： Glenn K. Fu ,  Stephen P. A. Fodor ,  Julie Wilhelmy

    IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874

    Abstract: Methods, kits and systems are disclosed for analyzing one or more molecules in a sample. Analyzing the one or more molecules may comprise quantitation of the one or more molecules. Individual molecules may quantitated by PCR, arrays, beads, emulsions, droplets, or sequencing. Quantitation of individual molecules may further comprise stochastic labeling of the one or more molecules with a plurality of oligonucleotide tags to produce one or more stochastically labeled molecules. The methods may further comprise amplifying, sequencing, detecting, and/or quantifying the stochastically labeled molecules. The molecules may be DNA, RNA and/or proteins.

    MOLECULAR LABEL COUNTING ADJUSTMENT METHODS
    25.
    发明申请
    MOLECULAR LABEL COUNTING ADJUSTMENT METHODS 审中-公开

    公开(公告)号：US20170344866A1

    公开(公告)日：2017-11-30

    申请号：US15605874

    申请日：2017-05-25

    Applicant: Cellular Research, Inc.

    Inventor： Jue Fan ,  Jennifer Tsai ,  Eleen Shum ,  Lisha Deng ,  Glenn K. Fu

    IPC: G06K19/06

    CPC classification number: G06K19/06028 ,  G16B30/00 ,  G16B40/00

    Abstract: Disclosed herein are methods and systems for determining the numbers of targets. In some embodiments, the method comprise: stochastically barcoding targets using stochastic barcodes; obtaining sequencing data; for one or more of the targets: counting the number of molecular labels with distinct sequences associated with the target in the sequencing data; identifying clusters of molecular labels of the target using directional adjacency; collapsing the sequencing data using the clusters of molecular labels of the target identified; and estimating the number of the target.

    Digital Counting of Individual Molecules by Stochastic Attachment of Diverse Labels
    28.
    发明申请
    Digital Counting of Individual Molecules by Stochastic Attachment of Diverse Labels 有权
    通过随机附加不同标签对个体分子进行数字计数

    公开(公告)号：US20150005185A1

    公开(公告)日：2015-01-01

    申请号：US14281706

    申请日：2014-05-19

    Applicant: Cellular Research, Inc.

    Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

    Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

    Abstract translation: 公开了组合物，方法和试剂盒，用于通过附加不同标签组来随机标记相同分子的集合来进行高灵敏度单分子数字计数。 分子的每个拷贝从不同标签的非消耗性储库中随机选择。 检测可以通过多种方法，包括基于杂交或测序。 否则在信息内容中相同的分子可以被标记以产生在集合中是独特的或大致唯一的可单独检测的产物。 这种随机变换放宽了从定位和识别相同分子之一计数分子的问题到检测是否存在预编程标签的一系列二进制数字问题。 该方法可以用于例如估计样品中给定类型或类型的单独分子的数目。

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