公开(公告)号：US20090169658A1

公开(公告)日：2009-07-02

申请号：US11965795

申请日：2007-12-28

申请人： Mei-Ling Ho ,  Hseng-Kuang Hsu ,  Gwo-Jaw Wang ,  Je-Ken Chang

发明人： Mei-Ling Ho ,  Hseng-Kuang Hsu ,  Gwo-Jaw Wang ,  Je-Ken Chang

IPC分类号： A61K36/58 ,  C12N5/00 ,  A61P35/04

CPC分类号： A61K36/58

摘要： Toona sinensis extract for suppressing the proliferation and inducing apoptosis of osteosarcoma, but not normal human osterblasts. The extraction process comprises: extracting Toona sinensis with water to obtain a first extract, and filtering the first extract by a membrane to obtain a filtrate, and the Toona sinensis extract of the invention does not cause biological damages of normal bone cells. In addition, the invention further provides a pharmaceutical composition comprising the Toona sinensis extract.

摘要翻译： 香椿提取物用于抑制骨肉瘤的增殖和诱导凋亡，但不是正常的人成骨细胞。 提取方法包括：用水提取香茅，得到第一提取物，用膜过滤第一提取物得到滤液，本发明的香椿提取物不会引起正常骨细胞的生物损伤。 此外，本发明还提供了包含香椿提取物的药物组合物。

公开(公告)号：US20080026362A1

公开(公告)日：2008-01-31

申请号：US11490860

申请日：2006-07-20

申请人： Mei-Ling Ho ,  Gwo-Jaw Wang ,  Je-Ken Chang

发明人： Mei-Ling Ho ,  Gwo-Jaw Wang ,  Je-Ken Chang

IPC分类号： C12Q1/00 ,  C12N5/08

CPC分类号： C12N5/0655 ,  A61K2035/126 ,  C12N2501/06 ,  C12N2533/74 ,  G01N33/5044 ,  G01N2800/105

摘要： Disclosed herein is a three-dimensional culture containing human articular chondrocytes with induced terminal differentiation changes, as well as a process for preparing the same. The three-dimensional culture, which was found to mimic the biological characteristics of articular chondrocytes that undergo terminal differentiation in vivo, can be used as a tool in the studies of the molecular and cellular mechanisms of osteoarthritis, and in the screening of candidate drugs for use in treatment of a disorder associated with articular chondrocytes.

摘要翻译： 本文公开了含有诱导末端分化改变的人关节软骨细胞的三维培养物及其制备方法。 被发现模仿体内终末分化的关节软骨细胞的生物学特性的三维培养物可用作骨关节炎分子和细胞机制研究中的工具，以及候选药物的筛选 用于治疗与关节软骨细胞相关的病症。

公开(公告)号：US08470522B2

公开(公告)日：2013-06-25

申请号：US11490860

申请日：2006-07-20

申请人： Mei-Ling Ho ,  Gwo-Jaw Wang ,  Je-Ken Chang

发明人： Mei-Ling Ho ,  Gwo-Jaw Wang ,  Je-Ken Chang

IPC分类号： C12Q1/00 ,  C12N15/07 ,  A61K35/32

CPC分类号： C12N5/0655 ,  A61K2035/126 ,  C12N2501/06 ,  C12N2533/74 ,  G01N33/5044 ,  G01N2800/105

摘要： Disclosed herein is a three-dimensional culture containing human articular chondrocytes with induced terminal differentiation changes, as well as a process for preparing the same. The three-dimensional culture, which was found to mimic the biological characteristics of articular chondrocytes that undergo terminal differentiation in vivo, can be used as a tool in the studies of the molecular and cellular mechanisms of osteoarthritis, and in the screening of candidate drugs for use in treatment of a disorder associated with articular chondrocytes.

摘要翻译： 本文公开了含有诱导末端分化改变的人关节软骨细胞的三维培养物及其制备方法。 被发现模仿体内终末分化的关节软骨细胞的生物学特性的三维培养物可用作骨关节炎分子和细胞机制研究中的工具，以及候选药物的筛选 用于治疗与关节软骨细胞相关的病症。

公开(公告)号：US20090170200A1

公开(公告)日：2009-07-02

申请号：US12106453

申请日：2008-04-21

申请人： Ching-Hua Yeh ,  Gwo-Jaw Wang ,  Mei-Ling Ho ,  Je-Ken Chang ,  Chung-Hwan Chen

发明人： Ching-Hua Yeh ,  Gwo-Jaw Wang ,  Mei-Ling Ho ,  Je-Ken Chang ,  Chung-Hwan Chen

IPC分类号： C12N5/06

CPC分类号： C12N5/0663 ,  C12N2500/12 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39

摘要： A medium for culturing stem cell. The stem cell medium of the invention comprises a fetal bovine serum, one or plurality of amino acid, one or plurality of vitamin, one or plurality of growth factor, one or plurality of inorganic salt, one or plurality of antioxidant, wherein the stem cell medium has a calcium concentration of less than about 1.8 mM, and the fetal bovine serum is present in an amount of less than about 10% by volume of the medium. The stem cell medium of the invention can maintain the proliferative and self-renewal capacity of the stem cells and keep stem cells at a steady stage.

摘要翻译： 培养干细胞的培养基。 本发明的干细胞培养基包含胎牛血清，一种或多种氨基酸，一种或多种维生素，一种或多种生长因子，一种或多种无机盐，一种或多种抗氧化剂，其中所述干细胞 培养基具有小于约1.8mM的钙浓度，并且胎牛血清以小于培养基体积的约10体积％的量存在。 本发明的干细胞培养基可以维持干细胞的增殖和自我更新能力，并使干细胞保持稳定。

公开(公告)号：US20090170177A1

公开(公告)日：2009-07-02

申请号：US12106436

申请日：2008-04-21

申请人： Mei-Ling Ho ,  Gwo-Jaw Wang ,  Je-Ken Chang ,  Yan-Hsiung Wang

发明人： Mei-Ling Ho ,  Gwo-Jaw Wang ,  Je-Ken Chang ,  Yan-Hsiung Wang

IPC分类号： C12N13/00

CPC分类号： C12N13/00 ,  C12N15/87

摘要： Stem cell transfection method. The stem cell infection method of the invention comprises providing a stem cell; positioning the stem cell at a buffer, wherein the buffer contains a foreign material; electroporating the stem cell in the buffer; and culturing the stem cell.

摘要翻译： 干细胞转染方法。 本发明的干细胞感染方法包括提供干细胞; 将干细胞定位在缓冲液中，其中缓冲液含有异物; 电穿孔缓冲液中的干细胞; 并培养干细胞。

公开(公告)号：US20090215768A1

公开(公告)日：2009-08-27

申请号：US12243376

申请日：2008-10-01

申请人： Cherng-Chyi Tzeng ,  Yeh-Long Chen ,  Gwo-Jaw Wang ,  Mai-Ling Ho ,  Je-Ken Chang ,  Yin-Chih Fu

发明人： Cherng-Chyi Tzeng ,  Yeh-Long Chen ,  Gwo-Jaw Wang ,  Mai-Ling Ho ,  Je-Ken Chang ,  Yin-Chih Fu

IPC分类号： A61K31/5377 ,  C07D261/06 ,  A61K31/42 ,  A61K31/496 ,  C07D413/02 ,  C07D413/12

CPC分类号： C07D261/08

摘要： An isoxazole derivative is provided. The isoxazole derivative has following formula: wherein R1, R2, R3, R4 and R5, independently, include hydrogen, hydroxy or C1-C12 alkoxy optionally substituted with oxirane, thiirane, aziridine, amino, cycloamino, aminohydroxy or cycloaminohydroxy. The invention also provides a pharmaceutical composition for treatment of osteoporosis and cancer including an isoxazole derivative or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.

摘要翻译： 提供异恶唑衍生物。 异恶唑衍生物具有下式：其中R 1，R 2，R 3，R 4和R 5独立地包括任选被环氧乙烷，硫杂环丁烷，氮丙啶，氨基，环氨基，氨基羟基或环氨基羟基取代的氢，羟基或C 1 -C 12烷氧基。 本发明还提供了用于治疗骨质疏松症和癌症的药物组合物，包括异恶唑衍生物或其药学上可接受的盐和药学上可接受的载体。

公开(公告)号：US20120045831A1

公开(公告)日：2012-02-23

申请号：US12860377

申请日：2010-08-20

申请人： Mei-Ling Ho ,  Je-Ken Chang ,  Rajalakshmanan Eswaramoorthy ,  Shun-Cheng Wu

发明人： Mei-Ling Ho ,  Je-Ken Chang ,  Rajalakshmanan Eswaramoorthy ,  Shun-Cheng Wu

IPC分类号： C12N5/00 ,  C08G63/06

CPC分类号： C08G63/912 ,  C12N5/0667 ,  C12N2500/42 ,  C12N2501/999 ,  C12N2533/40 ,  C12N2537/10

摘要： The present invention relates to a bisphosphonates and synthetic polymeric carriers to form a sustained release system. The present invention also provides a method for preparing a sustained release system, comprising activation of the synthetic polymeric carriers and the cross linking reaction. The present invention further provides methods for enhancing stem cell differentiation into osteogenic lineage and chondrogenic lineage, which comprise culturing a population of stem cells in specific micro-environments.

摘要翻译： 本发明涉及形成持续释放系统的双膦酸盐和合成聚合物载体。 本发明还提供一种制备持续释放系统的方法，包括活化合成聚合物载体和交联反应。 本发明还提供了增强干细胞分化成成骨谱系和软骨形成谱系的方法，其包括在特定微环境中培养干细胞群体。

公开(公告)号：US20120202064A1

公开(公告)日：2012-08-09

申请号：US13446827

申请日：2012-04-13

申请人： Mei-Ling Ho ,  Je-Ken Chang ,  Rajalakshmanan Eswaramoorthy ,  Shun-Cheng Wu

发明人： Mei-Ling Ho ,  Je-Ken Chang ,  Rajalakshmanan Eswaramoorthy ,  Shun-Cheng Wu

IPC分类号： C08G63/91 ,  A61K9/14

CPC分类号： C08G63/912 ,  C08J3/14 ,  C08J9/26 ,  C08J2201/0446 ,  C08J2367/04 ,  C12N5/0667 ,  C12N2500/42 ,  C12N2501/999 ,  C12N2533/40 ,  C12N2537/10 ,  Y10T428/2982

摘要： A short term controlled release composition which comprises poly(lactic-co-glycolic acid) cross-linked alendronate (PLGA-ALN) is provided. The PLGA-ALN is constructed into 3D scaffolds (PLGA-ALN-3D) with pores size of 150-300 μm and average porosity of 85%, or microspheres (PLGA-ALN-M) with 50-100 μm in diameter. The released alendronate concentration is in the range of 5×10−7 M to 5×10−8 M.

摘要翻译： 提供了包含聚（乳酸 - 共 - 乙醇酸）交联阿仑膦酸盐（PLGA-ALN）的短期控释组合物。 将PLGA-ALN构建成孔径为150-300μm，平均孔隙率为85％的3D支架（PLGA-ALN-3D）或直径为50-100μm的微球（PLGA-ALN-M）。 释放的阿仑膦酸盐浓度在5×10-7M至5×10-8M的范围内。