DNA detection methods for site specific nuclease activity
    24.
    发明授权
    DNA detection methods for site specific nuclease activity 有权
    用于位点特异性核酸酶活性的DNA检测方法

    公开(公告)号:US09493844B2

    公开(公告)日:2016-11-15

    申请号:US14105223

    申请日:2013-12-13

    Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

    Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法,以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程,并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。

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