公开(公告)号：US20230295692A1

公开(公告)日：2023-09-21

申请号：US18161581

申请日：2023-01-30

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Lorenzo BERTI ,  Semyon KRUGLYAK ,  Matthew KELLINGER ,  Molly HE ,  Sinan ARSLAN ,  Junhua ZHAO ,  Michael PREVITE

IPC: C12Q1/6816 ,  C12Q1/6855

CPC classification number: C12Q1/6816 ,  C12Q1/6855

Abstract: Methods and systems for detecting the presence of a target nucleic acid sequence in one or more samples of a plurality of samples are described. The methods may comprise the use of linear barcoded nucleic acid probes that, upon hybridization to a target nucleic acid sequence, may be ligated to circularize the probe molecule, amplified, and sequenced. The use of a probe-specific barcode integrated into the nucleic acid probe molecule, and sample-specific barcodes that may be incorporated into the nucleic acid probe molecule or added during the amplification step, enable large-scale multiplexed assay and sample processing.

公开(公告)号：US20230203564A1

公开(公告)日：2023-06-29

申请号：US18166429

申请日：2023-02-08

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  Samantha SNOW ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE ,  Michael KIM ,  Hua YU ,  Yu-Hsien HWANG-FU ,  Marco TJIOE ,  Andrew BODDICKER ,  Mark AMBROSO ,  Tyler LOPEZ ,  Michael KLEIN ,  Virginia SAADE

IPC: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6853

CPC classification number: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6458 ,  C12Q1/6853 ,  C12Q2600/158

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US20230193354A1

公开(公告)日：2023-06-22

申请号：US17992564

申请日：2022-11-22

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  Samantha SNOW ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE ,  Michael KIM ,  Hua YU ,  Yu-Hsien HWANG-FU ,  Marco TJIOE ,  Andrew BODDICKER

IPC: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6853

CPC classification number: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6458 ,  C12Q1/6853 ,  C12Q2600/158

Abstract: The disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US20230065693A1

公开(公告)日：2023-03-02

申请号：US17947984

申请日：2022-09-19

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE ,  Ramreddy TIPPANA ,  Hua YU ,  William LIGHT ,  Junhua ZHAO

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting nucleic acid sequencing workflows, where the workflows include library preparation, immobilization and amplification of the library molecules to form immobilized template molecules, and sequencing the template molecules. In some embodiments, the compositions comprise reagents used to conduct nucleic acid sequencing workflows.

公开(公告)号：US20210387184A1

公开(公告)日：2021-12-16

申请号：US17218027

申请日：2021-03-30

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE ,  Sinan ARSLAN ,  Molly HE ,  Huizhen MAH ,  Lei SUN

IPC: B01L3/00 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806 ,  G01N21/64

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20210123098A1

公开(公告)日：2021-04-29

申请号：US17144945

申请日：2021-01-08

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve CHEN

IPC: C12Q1/6869 ,  C12Q1/6841

Abstract: Provided are methods and systems for analyzing nucleic acids in a biological sample in a manner that retains the spatial and/or cellular origin of the nucleic acids within the biological sample. Compositions and kits are also provided that enable the methods and systems of the instant disclosure.

公开(公告)号：US20200347443A1

公开(公告)日：2020-11-05

申请号：US16543351

申请日：2019-08-16

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE

IPC: C12Q1/6834 ,  C12Q1/6832 ,  C12Q1/6806 ,  B01J19/00

Abstract: Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.