公开(公告)号：US20060099147A1

公开(公告)日：2006-05-11

申请号：US11301050

申请日：2005-12-12

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61B10/00 ,  B32B17/02

CPC classification number: C12Q1/6816 ,  B82Y15/00 ,  C12Q1/6818 ,  G01N33/586 ,  G01N33/588 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  C12Q2563/161 ,  C12Q2563/107 ,  C12Q2523/107

Abstract: Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

公开(公告)号：US06811752B2

公开(公告)日：2004-11-02

申请号：US09855920

申请日：2001-05-15

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: B01L302

CPC classification number: B01L3/50853 ,  Y10T436/2575

Abstract: Provided is a device comprising a plurality of microchambers having a closed vented environment, wherein each microchamber is in operative communication with a filling port and a vent aperture. The device further comprises a base which is sandwiched between two liquid-impermeable membranes, with at least one of the membranes being gas permeable. Also provided is a method for introducing a fluid into a plurality of microchambers of the device, wherein each filling port is aligned with a pipette tip, and the fluid is introduced into and through the filling port. The fluid then flows along a fluid flow groove providing fluid flow communication between the filling port and the microchamber, and into the microchamber.

Abstract translation: 提供了一种包括具有闭合排气环境的多个微室的装置，其中每个微室与填充口和排气口处于可操作的连通状态。 该装置还包括夹在两个液体不可渗透膜之间的基底，其中至少一个膜是透气的。 还提供了一种用于将流体引入装置的多个微室中的方法，其中每个填充口与移液管尖端对齐，并且将流体引入并穿过填充口。 然后，流体沿着流体流槽流动，从而在填充口和微型仓之间提供流体流动连通，并进入微型仓库。

公开(公告)号：US06418338B1

公开(公告)日：2002-07-09

申请号：US09244369

申请日：1999-02-04

Applicant: Emilio Barbera-Guillem ,  Edward W. Martin, Jr.

Inventor： Emilio Barbera-Guillem ,  Edward W. Martin, Jr.

IPC: A61B500

CPC classification number: C07K16/18 ,  G01N33/567 ,  G01N33/57426 ,  G01N33/57484

Abstract: A method is provided for immune corrective surgical removal of lymphoid tissues containing shed tumor antigen in an individual. A detector molecule, with binding specificity for shed tumor antigen, is administered to an individual, and detected in the individual is the presence of lymphoid tissue which contains shed tumor antigen, as detected by the signal emitted by the detector molecule bound to shed tumor antigen. The lymphoid tissue, detected as containing shed tumor antigen, may then be surgically removed, thereby removing B cells, shed tumor antigen, and follicular dendritic cells involved in a pro-tumor immune response that are contained in the removed lymphoid tissue.

Abstract translation: 提供了一种用于免疫矫正手术去除个体中含有流出的肿瘤抗原的淋巴组织的方法。 向个体施用具有对流出的肿瘤抗原具有结合特异性的检测器分子，并且在个体中检测到存在包含流下的肿瘤抗原的淋巴组织，如通过结合到流出肿瘤抗原的检测器分子发射的信号所检测的那样 。 检测为含有脱落的肿瘤抗原的淋巴组织然后可以手术去除，从而去除参与去除的淋巴组织中包含的前肿瘤免疫应答的B细胞，脱落的肿瘤抗原和滤泡性树突状细胞。

公开(公告)号：US06261779B1

公开(公告)日：2001-07-17

申请号：US09437076

申请日：1999-11-09

Applicant: Emilio Barbera-Guillem ,  M. Bud Nelson ,  Stephanie L. Castro

Inventor： Emilio Barbera-Guillem ,  M. Bud Nelson ,  Stephanie L. Castro

IPC: C12P1934

CPC classification number: B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/682 ,  G01N33/54306 ,  G01N33/588 ,  Y10T436/143333 ,  C12Q2563/149 ,  C12Q2563/131 ,  C12Q2563/107

Abstract: Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to operably link to a molecular probe, and secondary dots comprise a plurality of polynucleotide strands which are complemetary to the plurality of polynucleotide strands of the primary dots. Also provided is a method for detecting the presence or absence of target molecule in a sample comprising operably linking primary dots to molecular probe, contacting the complex formed with the sample, contacting the sample with successive additions of secondary dots and primary dots. If target molecule is present in the sample, the primary dots and secondary dots will form dendrimers that can be detected by fluorescence emission.

Abstract translation: 提供了包含官能化纳米晶体的组合物和测定试剂盒，其具有从其延伸的多个已知序列的多核苷酸链; 其中主要点被用于可操作地连接到分子探针，并且次要点包括与主要点的多个多核苷酸链互补的多个多核苷酸链。 还提供了用于检测样品中靶分子存在或不存在的方法，其包括可操作地将原始点与分子探针连接，使与样品形成的复合物接触，使样品与次要点和原始点的连续添加接触。 如果样品中存在靶分子，则主要点和次要点将形成可通过荧光发射检测的树枝状大分子。

公开(公告)号：US06224866B1

公开(公告)日：2001-05-01

申请号：US09411116

申请日：1999-10-04

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61K39395

CPC classification number: C07K16/2896 ,  A61K39/395 ,  A61K2039/505 ,  A61K31/00 ,  A61K2300/00

Abstract: A method for treating a pro-tumor immune response in an individual having, or suspected of having, a pro-tumor immune response, by administering a therapeutically effective amount of an immunotherapeutic composition which binds to a determinant on B cells, resulting in B cell depletion including of B cells that may be involved in promotion of tumor progression. Also provided are immunotherapeutic compositions which can be used for treating a pro-tumor immune response.

Abstract translation: 一种通过施用治疗有效量的与B细胞上的决定簇结合的免疫治疗组合物来治疗具有或怀疑具有前肿瘤免疫应答的个体中的前肿瘤免疫应答的方法，导致B细胞 包括可能参与促进肿瘤进展的B细胞的消耗。 还提供了可用于治疗前肿瘤免疫应答的免疫治疗组合物。

公开(公告)号：US06194213B1

公开(公告)日：2001-02-27

申请号：US09458752

申请日：1999-12-10

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: G01N3353

CPC classification number: G01N33/92 ,  Y10T428/2984

Abstract: Provided is a method of fluorescence detection of lipid membranes using lipophilic, functionalized nanocrystals; and lipid membranes labeled by the lipophilic, functionalized nanocrystals. The method comprises contacting an effective amount of lipophilic, functionalized nanocrystals with the lipid membranes; exposing the labeled lipid membranes to an excitation light suitable for exciting the functionalized nanocrystals present in the labeled lipid membranes to emit a fluorescence emission. Also provided is a composition comprising a functionalized nanocrystal which is bound to a substrate in a living tissue.

Abstract translation: 提供了使用亲油官能化纳米晶体进行脂质膜的荧光检测的方法; 和通过亲脂官能化纳米晶体标记的脂质膜。 该方法包括使有效量的亲脂性官能化纳米晶体与脂质膜接触; 将标记的脂质膜暴露于适于激发存在于标记的脂质膜中的官能化纳米晶体以发射荧光发射的激发光。 还提供了包含与生物体组织中的底物结合的官能化纳米晶体的组合物。

公开(公告)号：US20060194030A1

公开(公告)日：2006-08-31

申请号：US11381396

申请日：2006-05-03

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: B32B3/00

CPC classification number: G07F7/086 ,  A61K9/167 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/588 ,  G06K19/06 ,  G07D7/1205 ,  Y10S428/916 ,  Y10T428/24802 ,  Y10T428/24893 ,  Y10T428/24901 ,  Y10T428/25 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2998

Abstract: An identifiable code pattern, a method of providing a carrier material with an identifiable code pattern, and a method for detecting an identifiable code pattern are based on applying water-soluble nanocrystals to carrier materials in a spatially arranged format that can be detected by exposing the identifiable code pattern to an excitation light source in exciting the water-soluble nanocrystals, and detecting any fluorescence peaks emitted from the excited water-soluble nanocrystals using a detection system. Provided are compositions comprising water-soluble quantum dot compounds which may be used to apply to carrier materials in a method of forming an identifiable code pattern that may be used to provide discrete, detectable signals which may be detected for purposes of identification and/or verification.

Abstract translation: 可识别的代码模式，提供具有可识别代码模式的载体材料的方法和用于检测可识别代码模式的方法基于将水溶性纳米晶体以空间排列的格式应用于载体材料，该格式可以通过将 在激发水溶性纳米晶体中激发光源的可识别代码图案，以及使用检测系统检测从激发的水溶性纳米晶体发射的任何荧光峰。 提供了包含水溶性量子点化合物的组合物，其可以用于形成可识别码型的方法中应用于载体材料，所述方法可用于提供离散的，可检测的信号，其可以用于鉴定和/或验证目的 。

公开(公告)号：US20060039990A1

公开(公告)日：2006-02-23

申请号：US10923237

申请日：2004-08-20

Applicant: Alberto Barrueta ,  Josu Elizundia ,  Emilio Barbera-Guillem

Inventor： Alberto Barrueta ,  Josu Elizundia ,  Emilio Barbera-Guillem

IPC: A61K35/16

CPC classification number: A61K35/16

Abstract: A biological tissue regenerative agent and method for preparing the same. The agent comprises the compounds that are usually found sequestered within platelets, along with platelet cytosolic components, and serum. The agent is prepared by preparing two quanta of blood. The first is clotted, the cells discarded and the serum retained. The second quantum undergoes concentration and lysis of the platelets therein, followed by recombination of the lysed platelets and platelet internal products with serum to form the agent. In a preferred embodiment, lysis of platelets is accomplished by providing an effective amount of calcium. The agent may be further purified and may be frozen or freeze dried for storage.

Abstract translation: 一种生物组织再生剂及其制备方法。 该试剂包括通常发现在血小板内隔离的化合物，以及血小板胞质成分和血清。 该药剂通过制备两种血液量来制备。 首先凝结，弃去细胞，保留血清。 第二个量子在其中进行血小板的浓缩和裂解，然后裂解的血小板和血小板内部产物与血清重组以形成试剂。 在优选的实施方案中，通过提供有效量的钙来完成血小板的溶解。 可以进一步纯化试剂并将其冷冻或冷冻干燥以储存。

公开(公告)号：US20050191313A1

公开(公告)日：2005-09-01

申请号：US11097873

申请日：2005-04-01

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: A61K39/395 ,  C07K16/28 ,  A61K39/00 ,  A61K39/385

CPC classification number: C07K16/2896 ,  A61K39/395 ,  A61K2039/505 ,  A61K2300/00

Abstract: Provided are vaccines, methods of making the vaccines and methods for administering the vaccines for immunotherapy of an individual bearing, or at risk for developing, solid nonlymphoid tumor. The vaccine comprises an immunotherapeutic composition and tumor-associated antigen, and may further comprise one or more of an immunomodulator or a pharmaceutically acceptable carrier. A method of immunotherapy of an individual comprises administering to the individual an amount of the vaccine effective to suppress a TH2 response, and to induce a TH1 response against solid nonlymphoid tumor, in an individual having a TH2/TH1 imbalance.

Abstract translation: 提供疫苗，制备疫苗的方法和用于施用个体携带者的免疫治疗疫苗，或处于发展中的固体非淋巴瘤肿瘤的风险的方法。 疫苗包含免疫治疗组合物和肿瘤相关抗原，并且还可以包含免疫调节剂或药学上可接受的载体中的一种或多种。 个体的免疫治疗方法包括在具有TH2 / TH1不平衡的个体中给予个体一定量的有效抑制TH2应答的疫苗，并诱导针对固体非淋巴瘤肿瘤的TH1应答。

公开(公告)号：US06673595B2

公开(公告)日：2004-01-06

申请号：US09940388

申请日：2001-08-27

Applicant: Emilio Barbera-Guillem

Inventor： Emilio Barbera-Guillem

IPC: C12M136

CPC classification number: C12M41/14 ,  C12M23/24 ,  C12M23/48 ,  C12M23/50 ,  C12M41/46 ,  C12M41/48

Abstract: An automated cell management system which can be programmed to perform and control various operations of the essential phases of cell culturing, of cell culture manipulation, and of cell culture evaluation. The automated cell management system comprises a housing, a storage array for accommodating a plurality of cell culture devices, a loading station, a means for harvesting one or more components from a cell culture device, and one or more processing stations. The automated cell management system may further comprise mechanism for tracking each cell culture device, a plurality of reservoirs, a centrifuge, a microprocessor, one or more evaluation stations, one or more means for sterilization, and a combination thereof.

Abstract translation: 一种自动化细胞管理系统，其可编程为执行和控制细胞培养，细胞培养操作和细胞培养评估的基本阶段的各种操作。 自动细胞管理系统包括壳体，用于容纳多个细胞培养装置的储存阵列，装载站，用于从细胞培养装置收获一个或多个组分的装置以及一个或多个处理站。 自动细胞管理系统还可以包括用于跟踪每个细胞培养装置，多个储存器，离心机，微处理器，一个或多个评估站，一个或多个灭菌装置及其组合的机构。