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    ENGINEERED ENZYME HAVING ACETOACETYL-COA HYDROLASE ACTIVITY, MICROORGANISMS COMPRISING SAME, AND METHODS OF USING SAME
    24.
    发明申请
    ENGINEERED ENZYME HAVING ACETOACETYL-COA HYDROLASE ACTIVITY, MICROORGANISMS COMPRISING SAME, AND METHODS OF USING SAME 有权
    具有乙酰胆碱-CA水解酶活性的工程化酶，包含其的微生物及其使用方法

    公开(公告)号：US20160208228A1

    公开(公告)日：2016-07-21

    申请号：US15023579

    申请日：2014-09-23

    Applicant: Avram Michael SLOVIC ,  BRASKEM S.A.

    Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

    IPC: C12N9/16

    CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

    Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

    Abstract translation: 本公开提供了能够介导乙酰乙酰辅酶A向乙酰乙酸酯转化的工程化酶，其与乙酰辅酶A不具有与乙酰辅酶A相同数量级的反应（例如，工程化酶具有特定的乙酰乙酰基 - CoA水解酶活性比其乙酰-CoA水解酶活性高至少10倍）。 另外，本公开提供包含本文公开的工程化酶的修饰的微生物及其使用方法。

    MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING 2-PROPANOL AND1-PROPANOL AND/OR 1,2-PROPANEDIOL
    25.
    发明申请
    MODIFIED MICROORGANISM AND METHODS OF USING SAME FOR PRODUCING 2-PROPANOL AND1-PROPANOL AND/OR 1,2-PROPANEDIOL 审中-公开
    改性微生物及其用于生产2-丙醇和1-丙醇和/或1,2-丙二醇的方法

    公开(公告)号：US20150064759A1

    公开(公告)日：2015-03-05

    申请号：US14478737

    申请日：2014-09-05

    Applicant: Braskem S.A.

    Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

    IPC: C12P7/18 ,  C12P7/04

    CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

    Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol.

    Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 一种或多种编码催化细胞溶质乙酰辅酶A转化为2-丙醇的途径中的一种或多种酶的多核苷酸; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产2-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

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