公开(公告)号：US20210009988A1

公开(公告)日：2021-01-14

申请号：US17036075

申请日：2020-09-29

Applicant: GENENTECH, INC.

Inventor： Shahram Misaghi ,  Masaru Ken Shiratori ,  Bradley Richard Snedecor ,  Michael W. Laird

IPC: C12N15/10 ,  C12N5/071 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure relates to methods, cells and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interests, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating pyruvate kinase muscle (PKM) expression (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the lactogenic activity of the cell, as well compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using the same.

公开(公告)号：US10421984B2

公开(公告)日：2019-09-24

申请号：US15951895

申请日：2018-04-12

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/22 ,  C07K16/40 ,  C07K16/28 ,  C07K16/32

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US20180080056A1

公开(公告)日：2018-03-22

申请号：US15816305

申请日：2017-11-17

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/40 ,  C07K16/32 ,  C07K16/28 ,  C07K16/22

CPC classification number: C12P21/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/14 ,  C12N9/1029 ,  C12N9/1085 ,  C12N15/70 ,  C12P21/02 ,  C12Y203/01046 ,  C12Y205/01006

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

公开(公告)号：US20160186227A1

公开(公告)日：2016-06-30

申请号：US15067646

申请日：2016-03-11

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/00 ,  C12N9/10 ,  C07K16/32 ,  C07K16/22 ,  C07K16/40

CPC classification number: C12P21/00 ,  C07K16/18 ,  C07K16/22 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/14 ,  C12N9/1029 ,  C12N9/1085 ,  C12N15/70 ,  C12P21/02 ,  C12Y203/01046 ,  C12Y205/01006

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

Abstract translation: 本发明涉及用于在细菌中重组蛋白质生产期间防止将正亮氨酸掺入蛋白质的方法和组合物。 本发明还提供了与本文提供的方法和组合物一起使用的微生物宿主细胞和核酸分子。

公开(公告)号：US20140081003A1

公开(公告)日：2014-03-20

申请号：US14031463

申请日：2013-09-19

Applicant: Genentech, Inc.

Inventor： Michael W. Laird ,  Karthik Veeravalli

IPC: C12P21/02 ,  C07K16/18 ,  C07K16/40 ,  C07K16/22

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

Abstract translation: 本发明涉及用于在细菌中重组蛋白质生产期间防止将正亮氨酸掺入蛋白质的方法和组合物。 本发明还提供了与本文提供的方法和组合物一起使用的微生物宿主细胞和核酸分子。