公开(公告)号：US20090142851A1

公开(公告)日：2009-06-04

申请号：US12325115

申请日：2008-11-28

申请人： Keisuke Shima ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Osamu Nishimura ,  Susumu Tsunasawa ,  Kazuhiro Sonomura

发明人： Keisuke Shima ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Osamu Nishimura ,  Susumu Tsunasawa ,  Kazuhiro Sonomura

IPC分类号： G01N33/68 ,  C07K1/14 ,  C12P21/06

CPC分类号： C07K1/1072 ,  C07K1/128 ,  G01N33/6821

摘要： The present invention provides a method for specifically recovering a C-terminal peptide fragment, and a method for easily determining the sequence of a C-terminal peptide fragment, which is difficult to be determined by a conventional method, with the use of a mass spectrometer, in particular a method capable of de novo sequencing of a C-terminal peptide fragment. A method for selectively recovering a C-terminal peptide of a protein, comprising the steps of: in a cleavage product of a protein containing a C-terminal peptide fragment (A) having an α-amino group but not having an ε-amino group and the other peptide fragments (B) having an α-amino group and an ε-amino group, selectively modifying the α-amino groups to obtain a C-terminal peptide fragment modified (A′) and the other peptide fragments modified (B′); and separating the C-terminal peptide fragment modified (A′) from the modified cleavage product by allowing a carrier to hold the other peptide fragments modified (B′) via the ε-amino group. A method for determining the amino acid sequence of a C-terminal peptide of a protein, comprising the steps of: selectively recovering a C-terminal peptide of a protein by the above method; and determining the amino acid sequence by subjecting a recovered C-terminal peptide fragment to mass spectrometry measurement.

摘要翻译： 本发明提供了特异性回收C末端肽片段的方法，以及通过使用质谱仪容易地测定难以用常规方法测定的C末端肽片段的序列的方法 ，特别是能够重新测序C-末端肽片段的方法。 一种选择性回收蛋白质C末端肽的方法，包括以下步骤：在含有具有α-氨基但不具有ε-氨基的C-末端肽片段（A）的蛋白质的切割产物中 和具有α-氨基和ε-氨基的其它肽片段（B），选择性地修饰α-氨基以获得修饰的C末端肽片段（A'）和修饰的其它肽片段（B' ）; 以及通过允许载体通过ε-氨基保持修饰的其它肽片段（B'），从修饰的切割产物中分离修饰的C末端肽片段（A'）。 一种确定蛋白质C-末端肽的氨基酸序列的方法，包括以下步骤：通过上述方法选择性地回收蛋白质的C-末端肽; 并通过对回收的C-末端肽片段进行质谱测量来测定氨基酸序列。

公开(公告)号：US20050221413A1

公开(公告)日：2005-10-06

申请号：US10518358

申请日：2003-04-03

申请人： Hiroki Kuyama ,  Eiji Ando ,  Osamu Nishimura

发明人： Hiroki Kuyama ,  Eiji Ando ,  Osamu Nishimura

IPC分类号： C07C313/08 ,  G01N33/68 ,  C12Q1/37 ,  C07K14/00 ,  G01N33/00

CPC分类号： G01N33/6848 ,  C07B2200/05 ,  C07C313/08 ,  G01N2458/15

摘要： A sulfenyl compound represented by the general formula: R—S—X (I)(wherein R represents an organic group having at least one constituent element labeled with an isotope, and X represents a leaving group); a labeling reagent comprising it; and a method of analyzing peptide using the labeling reagent. Preferably the organic group R comprises C, H, and N, and optionally O and/or P as the constituent element, and the isotope is a stable isotope selected from the group consisting of 2H, 13C, 15N, 17O, and 18O.

摘要翻译： 由通式R-S-X（I）（其中R表示具有至少一个由同位素标记的构成元素，X表示离去基团的有机基团）表示的亚磺酰基化合物; 包含它的标记试剂; 以及使用标记试剂分析肽的方法。 优选地，有机基团R包含C，H和N，以及任选的O和/或P作为构成元素，并且同位素是选自下组的稳定同位素：SUP， > 13℃，15℃，17℃和<！ - SIPO - > O。

公开(公告)号：US5360699A

公开(公告)日：1994-11-01

申请号：US52867

申请日：1993-04-23

申请人： Junichi Hibino ,  Eiji Ando

发明人： Junichi Hibino ,  Eiji Ando

IPC分类号： G03C1/685 ,  C07D491/10 ,  C07D491/107 ,  C09K9/02 ,  G11B7/244 ,  G03C1/73

CPC分类号： C07D491/10 ,  C09K9/02 ,  G11B7/244 ,  G11B7/2531 ,  Y10S430/146 ,  Y10S430/163

摘要： The present invention provides a photochromic material which comprises a spiropyran compound of formula (I): ##STR1## wherein R.sup.1 and R.sup.2 are independently alkyl groups each containing 1 to 30 carbon atoms and Y is a halogen.The photochromic material possesses absorption sensitivity at wavelengths in the length region of about 700 nm which is the oscillation range of a semi-conductor laser device, and possesses higher stability compared with conventional one when it becomes a colored form.

摘要翻译： 本发明提供一种光致变色材料，其包含式（I）的吡咯烷化合物：其中R 1和R 2各自独立地为含有1至30个碳原子的烷基，Y为卤素。 光致变色材料在半导体激光器件的振荡范围约700nm的长度区域的波长处具有吸收灵敏度，并且当成为有色形式时，其具有比常规的更高的稳定性。

公开(公告)号：US5241075A

公开(公告)日：1993-08-31

申请号：US869079

申请日：1992-04-14

申请人： Junichi Hibino ,  Eiji Ando

发明人： Junichi Hibino ,  Eiji Ando

IPC分类号： C07D209/08 ,  C07D491/10 ,  C07D491/107 ,  G03C1/685 ,  G11B7/244

CPC分类号： C07D491/10 ,  C07D209/08 ,  G03C1/685 ,  G11B7/244

摘要： Novel photochromic spiropyran compounds having an absorption sensitivity in a longer wavelength region than known photochromic compounds are provided. The novel spiropyran compounds have a spiropyran skeleton having a methoxy group at the 6 position, a nitro group at the 8 position, a bromine atom at the 5' and 7' positions, and an alkyl group at the 1' position.

摘要翻译： 提供了在比已知的光致变色化合物更长的波长区域具有吸收灵敏度的新型光致变色螺吡喃化合物。 新的螺吡喃化合物具有在6位具有甲氧基的螺吡喃骨架，8位的硝基，5'和7'位的溴原子和1'位的烷基。

公开(公告)号：US4803287A

公开(公告)日：1989-02-07

申请号：US023253

申请日：1987-03-09

申请人： Junichi Hibino ,  Eiji Ando

发明人： Junichi Hibino ,  Eiji Ando

IPC分类号： C07D307/02 ,  G11B7/0045 ,  G11B7/005 ,  G11B7/0055 ,  G11B7/244 ,  C07D307/60

CPC分类号： C07D307/02 ,  G11B7/00455 ,  G11B7/0052 ,  G11B7/00552 ,  G11B7/244

摘要： The invention provides a photochromic material characterized by an improvnt in balance of the substituents in the conventional furylfulgides and represented by the general formula: ##STR1## (wherein R represents an alkyl chain having 5 to 31 carbon atoms). According to this molecular structure, a mutually appropriate steric hindrance is induced between the isopropylidence group and the long-chain alkyl group to make the regio isomer unstable, thus preventing the regio isomer from being by-produced while also inhibiting any isomerization reaction from occuring in the synthesis of the fulgide. Further, a good balance of hydrophilic part and hydrophobic part is produced to facilitate formation of a Langmuir-Blodgett film.

摘要翻译： 本发明提供了一种光致变色材料，其特征在于改善常规呋喃草酮中取代基的平衡，并由以下通式表示：其中R表示具有5至31个碳原子的烷基链。 根据该分子结构，在异丙基化基团和长链烷基之间引入相互适当的空间位阻，使得区域异构体不稳定，从而防止区域异构体副产物，同时还抑制异构化反应发生 该抗体的合成。 此外，产生亲水部分和疏水部分的良好平衡以促进Langmuir-Blodgett膜的形成。

公开(公告)号：US09708158B2

公开(公告)日：2017-07-18

申请号：US14380206

申请日：2012-04-16

申请人： Masayuki Kakio ,  Takuo Kugiya ,  Kiyoshi Funai ,  Eiji Ando

发明人： Masayuki Kakio ,  Takuo Kugiya ,  Kiyoshi Funai ,  Eiji Ando

IPC分类号： B66B9/00 ,  B66B5/02 ,  B66B5/00

CPC分类号： B66B5/02 ,  B66B5/0031 ,  B66B9/00

摘要： In a multi-car elevator, if two vertically adjacent cars among the cars are designated first and second cars, then: a zone that is a distance in which the second car can be stopped in response to an abnormality, and into which the first car is not permitted to enter is set as an exclusion zone of the second car; a position before which it is necessary for the first car to stop is set as a stopping limit position of the first car; and a plurality of threshold values that progressively detect the abnormal approach are set so as to enable the first car to decelerate and stop before the stopping limit position of the first car.

公开(公告)号：US08186484B2

公开(公告)日：2012-05-29

申请号：US13236811

申请日：2011-09-20

申请人： Takaharu Ueda ,  Eiji Ando

发明人： Takaharu Ueda ,  Eiji Ando

IPC分类号： B66B1/34

CPC分类号： B66B5/288 ,  B66B5/0062 ,  B66B5/04 ,  B66B13/02

摘要： An elevator system in which a controller monitors whether or not a speed of an ascending/descending body reaches a preset overspeed. At a time of maintenance work, a stopper is interposed between a buffer and the ascending/descending body to limit a lowered position of the ascending/descending body. A stopper detector detects installation of the stopper onto at least one of the buffer and the ascending/descending body. When the stopper is detected by the stopper detector, the controller lowers a set value of the overspeed.

摘要翻译： 一种电梯系统，其中控制器监视上升/下降体的速度是否达到预设的超速。 在维护工作时，在缓冲器和升降体之间插入有止动件，以限制升降体的降低位置。 止动器检测器检测止动件到缓冲器和上升/下降体中的至少一个的安装。 当止动器检测器检测到止动器时，控制器降低超速设定值。

公开(公告)号：US20110092660A1

公开(公告)日：2011-04-21

申请号：US12863165

申请日：2009-01-14

申请人： Tadashi Okawa ,  Eiji Ando

发明人： Tadashi Okawa ,  Eiji Ando

IPC分类号： C08G77/04

CPC分类号： C08G77/38 ,  C08G77/12 ,  C08G77/46

摘要： A methacryloxy group- or acryloxy group-containing polyorganosiloxane in which a methacryloxy group or acryloxy group is bonded to a silicon atom in the polyorganosiloxane across a long-chain alkylene group or poly(alkyleneoxy)-long chain-alkylene group having 11 to 20 carbon atoms. A method of producing this methacryloxy group- or acryloxy group-containing polyorganosiloxane, in which a silicon-bonded hydrogen atom-containing polyorganosiloxane is addition reacted in the presence of a hydrosilylation reaction catalyst with a 1-alkenyl methacrylate or a 1-alkenyl acrylate or a 1-alkenyloxypolyalkylene glycol methacrylate or a 1-alkenyloxypolyalkylene glycol acrylate.

摘要翻译： 含有甲基丙烯酰氧基或丙烯酰氧基的聚有机硅氧烷，其中甲基丙烯酰氧基或丙烯酰氧基通过长链亚烷基或聚（亚烷氧基） - 长链亚烷基键合到聚有机硅氧烷中的硅原子上，其具有11至20碳 原子 在含硅键合的含氢原子的聚有机硅氧烷的加成反应催化剂的存在下，在氢化硅烷化反应催化剂与1-链烯基甲基丙烯酸酯或1-烯基丙烯酸酯的情况下生产该含甲基丙烯酰氧基或含丙烯酰氧基的聚有机硅氧烷的方法，或 1-链烯氧基聚亚烷基二醇甲基丙烯酸酯或1-链烯氧基聚亚烷基二醇丙烯酸酯。

公开(公告)号：US07579165B2

公开(公告)日：2009-08-25

申请号：US10962575

申请日：2004-10-13

申请人： Shigemi Norioka ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Takashi Obama ,  Eiji Ando ,  Takashi Nakazawa ,  Norikazu Ueyama ,  Taka-aki Okamura

发明人： Shigemi Norioka ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Takashi Obama ,  Eiji Ando ,  Takashi Nakazawa ,  Norikazu Ueyama ,  Taka-aki Okamura

IPC分类号： C07K1/13 ,  C07K1/14 ,  C07K14/00 ,  C12P21/06 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/37 ,  G01N33/53 ,  G01N33/38 ,  G01N33/483

CPC分类号： C12N9/2462 ,  C07K1/1077 ,  C07K1/128 ,  C07K14/78

摘要： The present invention provides a method for effectively introducing sulfonic acid groups into the N-terminus of a protein or a peptide. The method comprises a modification step to react a N-terminus in a protein or peptide with a compound A which includes disulfide group and a cleavage step to cleave a disulfide bond of the disulfide group to convert into a sulfonic acid group. The present invention also provides a method of analyzing proteins or peptides easily and effectively on mass spectrometry and an intermediate that can be used to effectively derivatize proteins or peptides as sulfonic acid derivatives.

摘要翻译： 本发明提供了将磺酸基团有效地引入蛋白质或肽的N-末端的方法。 该方法包括使蛋白质或肽中的N末端与包含二硫键的化合物A和切割二硫键的二硫键以切割成磺酸基团的切割步骤进行反应的修饰步骤。 本发明还提供了一种在质谱分析中容易且有效地分析蛋白质或肽的方法，以及可用于有效地将蛋白质或肽衍生为磺酸衍生物的中间体。

公开(公告)号：US5155230A

公开(公告)日：1992-10-13

申请号：US481871

申请日：1990-02-20

申请人： Junichi Hibino ,  Eiji Ando

发明人： Junichi Hibino ,  Eiji Ando

IPC分类号： C07D491/10 ,  C07D491/107 ,  C09K9/02 ,  G03C1/685

CPC分类号： C07D491/10 ,  G03C1/685

摘要： The present invention provides a photochromic material possessing absorption sensitivity in a longer wavelength region when compared with conventional photochromic materials. The photochromic material is a spiropyran compound having a methoxy group at the 6 position, nitro groups at the 8 and 5' positions, and an alkyl group at the 1' position of the spiropyran skeleton as shown below: ##STR1##