公开(公告)号：US20160168633A1

公开(公告)日：2016-06-16

申请号：US15009246

申请日：2016-01-28

Applicant: Illumina, Inc.

Inventor： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2537/149 ,  C12Q2561/12 ,  C12Q2565/518 ,  C12Q2565/537

Abstract: A system for determining nucleic acid sequences, including (a) an array of nucleic acid features; (b) a fluidic apparatus configured to deliver sequencing reagents, including polymerases and nucleotides, to the array; (c) a detector configured to obtain pre-equilibrium kinetic measurements from the array at a resolution that distinguishes individual nucleic acid features; (d) a control module having instructions for (i) directing the fluidic apparatus to deliver the sequencing reagents to the array, and (ii) directing the detection apparatus to obtain the kinetic measurements; and (e) an analysis module having instructions for (i) processing the kinetic measurements to determine binding of the polymerase molecules to the nucleic acid features at several pre-equilibrium time points, thereby determining a transient state of the polymerase molecules at the nucleic acid features, and (ii) identifying nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules.

Abstract translation: 一种用于测定核酸序列的系统，包括（a）核酸特征阵列; （b）流体装置，其配置为将测序试剂（包括聚合酶和核苷酸）递送到阵列; （c）检测器，被配置为以区分各个核酸特征的分辨率从阵列获得预平衡动力学测量; （d）控制模块，其具有用于（i）引导流体装置将测序试剂递送到阵列的指令，以及（ii）引导检测装置获得动力学测量; 和（e）具有以下指令的分析模块：（i）处理动力学测量以在几个预平衡时间点确定聚合酶分子与核酸特征的结合，从而确定核酸处的聚合酶分子的瞬态 特征，和（ii）鉴定基于聚合酶分子的瞬态的正确掺入核苷酸分子的核酸特征。

公开(公告)号：US09279154B2

公开(公告)日：2016-03-08

申请号：US13722979

申请日：2012-12-20

Applicant: ILLUMINA, INC.

Inventor： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2537/149 ,  C12Q2561/12 ,  C12Q2565/518 ,  C12Q2565/537

Abstract: A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

Abstract translation: 区分不同核酸分子的核苷酸序列的方法，包括以下步骤：（a）将多种不同的核酸分子与聚合酶分子和核苷酸分子混合，其中不同的核酸分子以阵列的形式附着在表面上 的核酸特征; （b）确定聚合酶分子在核酸特征处的瞬时状态; 和（c）鉴定核酸特征的子集，其核酸分子基于聚合酶分子在核酸特征处的瞬时状态正确地掺入，从而区分不同核酸分子的核苷酸序列。