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    Conformational probes and methods for sequencing nucleic acids
    1.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US12297498B2

    公开(公告)日：2025-05-13

    申请号：US18129747

    申请日：2023-03-31

    Applicant: Illumina, Inc.

    Inventor： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6874

    Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

    Conformational probes and methods for sequencing nucleic acids
    6.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US10233493B2

    公开(公告)日：2019-03-19

    申请号：US15853118

    申请日：2017-12-22

    Applicant: Illumina, Inc.

    Inventor： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

    Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

    PLATFORM FOR DISCOVERY AND ANALYSIS OF THERAPEUTIC AGENTS
    7.
    发明申请
    PLATFORM FOR DISCOVERY AND ANALYSIS OF THERAPEUTIC AGENTS 审中-公开

    公开(公告)号：US20180142378A1

    公开(公告)日：2018-05-24

    申请号：US15572718

    申请日：2016-05-09

    Applicant: Illumina, Inc.

    Inventor： Molly He ,  Michael Previte ,  Misha Golynskiy ,  Matthew William Kellinger ,  Sergio Peisajovich ,  Jonathan Mark Boutell

    IPC: C40B50/16 ,  B01J19/00 ,  B01L3/00 ,  C40B20/04

    Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.

    Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
    10.
    发明授权
    Apparatus and methods for kinetic analysis and determination of nucleic acid sequences 有权

    公开(公告)号：US11242560B2

    公开(公告)日：2022-02-08

    申请号：US16127067

    申请日：2018-09-10

    Applicant: Illumina, Inc.

    Inventor： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

    IPC: C12Q1/6874

    Abstract: A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

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