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    Conformational probes and methods for sequencing nucleic acids
    1.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US12297498B2

    公开(公告)日：2025-05-13

    申请号：US18129747

    申请日：2023-03-31

    Applicant: Illumina, Inc.

    Inventor： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6874

    Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

    Conformational probes and methods for sequencing nucleic acids
    4.
    发明授权
    Conformational probes and methods for sequencing nucleic acids 有权

    公开(公告)号：US10233493B2

    公开(公告)日：2019-03-19

    申请号：US15853118

    申请日：2017-12-22

    Applicant: Illumina, Inc.

    Inventor： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

    IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

    Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

    Nucleotides and primers with removable blocking groups
    6.
    发明授权
    Nucleotides and primers with removable blocking groups 有权
    具有可除去阻断基团的核苷酸和引物

    公开(公告)号：US09150896B2

    公开(公告)日：2015-10-06

    申请号：US13782066

    申请日：2013-03-01

    Applicant: Illumina, Inc.

    Inventor： Cheng-Yao Chen ,  Gothami Padmabandu

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12P19/34 ,  C12Q1/6848 ,  C12Q2523/107 ,  C12Q2525/186 ,  C12Q2563/137 ,  C12Q2549/101

    Abstract: Provided herein is a method of amplifying nucleic acids using a plurality of modified nucleotides one or more of the nucleotides comprising a 3′ blocking group. Also provided is a method of amplifying nucleic acids using oligonucleotide primers one or both of the primers comprising a 3′ blocking group on one or more of the nucleotides of the primers.

    Abstract translation: 本文提供了使用多个修饰的核苷酸扩增核酸的方法，所述核苷酸包含3'阻断基团的一个或多个核苷酸。 还提供了使用寡核苷酸引物扩增核酸的方法，所述引物在引物的一个或多个核苷酸上包含3'封闭基团的一个或两个引物。

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