公开(公告)号：US20210095243A1

公开(公告)日：2021-04-01

申请号：US17026359

申请日：2020-09-21

Applicant: Inscripta, Inc.

Inventor： Bryan Leland ,  Michael Clay ,  Eric Abbate ,  Stephen Federowicz ,  Richard Fox

IPC: C12N1/14 ,  C12N15/10 ,  C12N9/10

Abstract: The present disclosure relates to various different types of mutations in S. cerevisiae leading to enhanced tyrosine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US20210002628A1

公开(公告)日：2021-01-07

申请号：US16988696

申请日：2020-08-09

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US10774446B1

公开(公告)日：2020-09-15

申请号：US16904640

申请日：2020-06-18

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C40B50/06 ,  C40B70/00 ,  C40B40/10 ,  C12N15/90 ,  C12N15/85 ,  C40B20/04 ,  C40B30/04 ,  C12N9/22 ,  C07K16/28 ,  C40B40/02 ,  C07K14/74 ,  C12N15/81

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US10774324B2

公开(公告)日：2020-09-15

申请号：US16670396

申请日：2019-10-31

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81 ,  C40B50/04 ,  C40B40/02

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US20200216977A1

公开(公告)日：2020-07-09

申请号：US16827164

申请日：2020-03-23

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US11359187B1

公开(公告)日：2022-06-14

申请号：US17680279

申请日：2022-02-24

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US11286471B1

公开(公告)日：2022-03-29

申请号：US17526951

申请日：2021-11-15

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20210355467A1

公开(公告)日：2021-11-18

申请号：US17388358

申请日：2021-07-29

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/62 ,  C12N15/11 ,  C12N15/70

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20200063288A1

公开(公告)日：2020-02-27

申请号：US16670340

申请日：2019-10-31

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C40B70/00 ,  C40B40/02 ,  C40B20/04 ,  C12N15/90 ,  C12N15/85 ,  C12N15/81 ,  C12N15/10 ,  C07K14/74 ,  C07K16/28 ,  C40B40/10 ,  C40B30/04 ,  C12N9/22

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US10557216B2

公开(公告)日：2020-02-11

申请号：US16392605

申请日：2019-04-23

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.