公开(公告)号：US20220389461A1

公开(公告)日：2022-12-08

申请号：US17752122

申请日：2022-05-24

Applicant: Inscripta, Inc.

Inventor： Randy Lacey ,  Bryan Leland ,  Eileen Spindler ,  Patrick Westfall ,  Skylar Stefani ,  Catherine Baranowski

IPC: C12N15/90 ,  G16B20/20

Abstract: The present disclosure relates to automated multi-module instruments, compositions and methods for performing nucleic acid-guided nuclease editing; specifically, methods, instruments, systems, and nucleic acids synthetic cassettes that improve the efficiency of gene editing using CRISPR enzymes in diploid cells—either on both chromosomes or selectively in one chromosome—without incurring loss of heterozygosity (LOH) and its often deleterious effects.

公开(公告)号：US20220002755A1

公开(公告)日：2022-01-06

申请号：US17363942

申请日：2021-06-30

Applicant: Inscripta, Inc.

Inventor： Stephen Tanner ,  Michael Graige ,  Craig Struble ,  Nandini Krishnamurthy ,  Andrea Halweg-Edwards ,  Bryan Leland ,  Simon Cawley ,  Joshua Shorenstein ,  Timothy Shaver

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation to make auxiliary or ancillary edits—in addition to intended edits and immunizing edits—in cellular genomic nucleic acids in order to assess and improve nucleic acid-guided nuclease or nickase fusion editing.

公开(公告)号：US20210095243A1

公开(公告)日：2021-04-01

申请号：US17026359

申请日：2020-09-21

Applicant: Inscripta, Inc.

Inventor： Bryan Leland ,  Michael Clay ,  Eric Abbate ,  Stephen Federowicz ,  Richard Fox

IPC: C12N1/14 ,  C12N15/10 ,  C12N9/10

Abstract: The present disclosure relates to various different types of mutations in S. cerevisiae leading to enhanced tyrosine production for, e.g., supplements and nutraceuticals.