公开(公告)号：US5198354A

公开(公告)日：1993-03-30

申请号：US699145

申请日：1991-05-13

申请人： Geoffrey G. Wilson

发明人： Geoffrey G. Wilson

IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/55

CPC分类号： C12N9/1007 ,  C12N9/22

摘要： Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening to identify those clones containing the restriction gene.

摘要翻译： 通过选择通过限制酶对体外消化有抗性的克隆并随后进行筛选以鉴定含有限制性基因的克隆来克隆限制酶及其相应修饰酶的方法。

公开(公告)号：US5196331A

公开(公告)日：1993-03-23

申请号：US699148

申请日：1991-05-13

申请人： Geoffrey G. Wilson

发明人： Geoffrey G. Wilson

IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/55

CPC分类号： C12N9/1007 ,  C12N9/22

摘要： Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening to identify those clones containing the restriction gene.

摘要翻译： 通过选择通过限制酶对体外消化有抗性的克隆并随后进行筛选以鉴定含有限制性基因的克隆来克隆限制酶及其相应修饰酶的方法。

公开(公告)号：US5180673A

公开(公告)日：1993-01-19

申请号：US323114

申请日：1989-03-13

申请人： Geoffrey G. Wilson ,  Donald Nwankwo

发明人： Geoffrey G. Wilson ,  Donald Nwankwo

IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/55

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening to identify those clones containing the restriction gene.

摘要翻译： 通过选择通过限制酶对体外消化有抗性的克隆并随后进行筛选以鉴定含有限制性基因的克隆来克隆限制酶及其相应修饰酶的方法。

公开(公告)号：US5179015A

公开(公告)日：1993-01-12

申请号：US557329

申请日：1990-07-23

申请人： Geoffrey G. Wilson ,  Marta M. Meda

发明人： Geoffrey G. Wilson ,  Marta M. Meda

IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/55

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： The present invention provides a novel approach to the production of restriction enzymes. More specifically, there is provided a novel method for cloning these enzymes, which comprises preparing DNA libraries from the DNA of an organism that synthesizes the restriction enzyme of interest, creating a suitable host containing a heterospecific methyltransferase gene able to protectively modify DNA from digestion by the restriction enzyme of interest, introducing the DNA libraries into the protectively modified host, and screening recombinant organisms to identify those carrying the desired restriction enzyme gene.The application of this method to the FspI and HaeIII restriction genes of Fischerella species and Haemophilus aegyptius, respectively, is described in detail, together with the resulting clones that form the basis of a new and useful process for purifying the FspI and HaeIII restriction enzymes.

摘要翻译： 本发明提供了一种制备限制酶的新方法。 更具体地，提供了一种用于克隆这些酶的新方法，其包括从合成所关注的限制酶的生物体的DNA制备DNA文库，产生含有能够通过以下方式保护性地修饰DNA免于消化的异源甲基转移酶基因的合适的宿主 将所述限制酶引入保护性修饰的宿主中，并筛选重组生物以鉴定携带所需限制酶基因的那些。 将这种方法分别应用于Fischerella物种和嗜血杆菌的FspI和HaeIII限制性基因，以及所得到的克隆构成了用于纯化FspI和HaeIII限制酶的新的和有用的方法的基础。

公开(公告)号：US4983542A

公开(公告)日：1991-01-08

申请号：US136093

申请日：1987-12-21

申请人： Elizabeth M. Van Cott ,  Geoffrey G. Wilson

发明人： Elizabeth M. Van Cott ,  Geoffrey G. Wilson

IPC分类号： C12N9/16 ,  C12N1/21 ,  C12N9/10 ,  C12N9/22 ,  C12N15/00 ,  C12N15/09 ,  C12N15/51 ,  C12R1/19 ,  C12R1/64

CPC分类号： C12N9/1007 ,  C12N9/22

摘要： The present invention is directed to a method for cloning and producing the XbaI restriction endonuclease by (1) introducing the restriction endonuclease gene from Xanthomonas badrii into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the XbaI restriction endonuclease, and (3) purifying the XbaI restriction endonuclease from the fermented host which contains the vector encoding and expressing the XbaI restriction endonuclease activity.