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    Polymerase compositions and kits, and methods of using and making the same
    21.
    发明授权
    Polymerase compositions and kits, and methods of using and making the same 有权

    公开(公告)号：US11001814B2

    公开(公告)日：2021-05-11

    申请号：US16415063

    申请日：2019-05-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Peter Vander Horn ,  Eileen Tozer ,  Sihong Chen ,  Guobin Luo ,  Joshua Shirley ,  Kevin Heinemann

    IPC: C12N9/12 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Polymerase compositions and methods of making and using same
    22.
    发明授权
    Polymerase compositions and methods of making and using same 有权

    公开(公告)号：US10745747B2

    公开(公告)日：2020-08-18

    申请号：US15961206

    申请日：2018-04-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Polymerase compositions, methods of making and using same
    23.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US10676724B2

    公开(公告)日：2020-06-09

    申请号：US16270129

    申请日：2019-02-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/6869 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Sequencing methods, compositions and systems using terminator nucleotides
    24.
    发明授权
    Sequencing methods, compositions and systems using terminator nucleotides 有权

    公开(公告)号：US10544455B2

    公开(公告)日：2020-01-28

    申请号：US14856220

    申请日：2015-09-16

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GmbH

    Inventor： Wolfgang Hinz ,  Peter Vander Horn ,  Earl Hubbell ,  Christian Woehler

    IPC: C12P19/34 ,  C12Q1/6869

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.

    Polymerase compositions and kits, and methods of using and making the same
    25.
    发明授权
    Polymerase compositions and kits, and methods of using and making the same 有权

    公开(公告)号：US10344268B2

    公开(公告)日：2019-07-09

    申请号：US15277834

    申请日：2016-09-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Peter Vander Horn ,  Eileen Tozer ,  Sihong Chen ,  Guobin Luo ,  Joshua Shirley ,  Kevin Heinemann

    IPC: C12N9/12 ,  C12Q1/686

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

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