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    Polymerase compositions and kits, and methods of using and making the same
    2.
    发明授权
    Polymerase compositions and kits, and methods of using and making the same 有权

    公开(公告)号：US11866740B2

    公开(公告)日：2024-01-09

    申请号：US17302621

    申请日：2021-05-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Peter B. Vander Horn ,  Eileen Tozer ,  Sihong Chen ,  Guobin Luo ,  Joshua Shirley ,  Kevin Heinemann

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q1/686 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Polymerase compositions and methods of making and using same
    4.
    发明授权
    Polymerase compositions and methods of making and using same 有权

    公开(公告)号：US12215383B2

    公开(公告)日：2025-02-04

    申请号：US17725369

    申请日：2022-04-20

    Applicant: Life Technologies Corporation

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME
    6.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME 有权

    公开(公告)号：US20220307071A1

    公开(公告)日：2022-09-29

    申请号：US17725369

    申请日：2022-04-20

    Applicant: Life Technologies Corporation

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12Q1/686 ,  C12Q1/6869 ,  C12N9/12

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Polymerase compositions and methods of making and using same
    7.
    发明授权
    Polymerase compositions and methods of making and using same 有权

    公开(公告)号：US11319584B2

    公开(公告)日：2022-05-03

    申请号：US16947742

    申请日：2020-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Library preparation methods and compositions and uses therefor
    8.
    发明授权
    Library preparation methods and compositions and uses therefor 有权

    公开(公告)号：US11001836B2

    公开(公告)日：2021-05-11

    申请号：US16024175

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Daniel Mazur ,  Sihong Chen ,  Guobin Luo ,  Xinzhan Peng

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B20/04 ,  C40B50/06 ,  C40B40/06

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    COMPOSITIONS AND METHODS FOR MULTIPLEX RT-PCR AND GENETIC ANALYSIS
    10.
    发明申请
    COMPOSITIONS AND METHODS FOR MULTIPLEX RT-PCR AND GENETIC ANALYSIS 有权

    公开(公告)号：US20220403446A1

    公开(公告)日：2022-12-22

    申请号：US17638435

    申请日：2020-08-28

    Applicant: Life Technologies Corporation

    Inventor： Sihong Chen

    IPC: C12Q1/686

    Abstract: The present invention is directed to compositions, methods and kits useful for the generation of nucleic acids from RNA templated and further nucleic acid amplification and detection. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase polymerase chain reaction (RT-PCR). Provided are compositions and methods for improved amplification of nucleic acid molecules in a two-step, addition only RT-PCR procedure. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules, and is useful for a variety of research, industrial, medical and forensic purposes.

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