公开(公告)号：US20230395195A1

公开(公告)日：2023-12-07

申请号：US18237358

申请日：2023-08-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daqing WANG ,  Pius Brzoska ,  Elliot Shelton

IPC: G16B40/20 ,  G06N20/10 ,  G16B20/00 ,  C12Q1/686

CPC classification number: G16B40/20 ,  C12Q1/686 ,  G16B20/00 ,  G06N20/10

Abstract: A quality control system for a qPCR receives signals resulting from operation of the qPCR system on an assay, and applies labeled data sets to a Support Vector Machine (SVM) to generate classifications for the signals to generate classifications that are utilized as operational feedback to the qPCR system.

公开(公告)号：US11008621B2

公开(公告)日：2021-05-18

申请号：US15128076

申请日：2015-03-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David Merrill ,  Pius Brzoska ,  Zheng Li ,  Wendy Lin ,  Wing Lee ,  Mandi Wong

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6851 ,  C07H21/04

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.

公开(公告)号：US09255299B2

公开(公告)日：2016-02-09

申请号：US13785409

申请日：2013-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性和差异性检测鸟分枝杆菌亚种的组合物，测定法，方法，诊断方法，试剂盒和诊断试剂盒。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（例如土壤，污垢，垃圾，污水，空气或水）的样品中的副结核病，包括食品加工和制造表面或生物样品。

公开(公告)号：US08906628B2

公开(公告)日：2014-12-09

申请号：US13870779

申请日：2013-04-25

Applicant: Life Technologies Corporation

Inventor： Paolo Vatta ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/689 ,  C12Q2600/142

Abstract: Disclosed are methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.

Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而不是大肠杆菌O55：H7的方法和试剂盒，来自样品如：复合食物基质，水，饮料，发酵液，法医和生物样品以及包括食物在内的环境样品 加工和制造表面。 在一些实施方案中，本公开的方法包括：使至少第一对多核苷酸引物与至少第一靶多核苷酸序列杂交，将至少第二对多核苷酸引物与至少第二靶多核苷酸序列杂交， 至少第一和至少第二靶多核苷酸序列，以及检测第一和第二扩增的靶多核苷酸序列产物，其中第一扩增的目标多核苷酸序列产物和第二扩增的靶多核苷酸序列产物的检测指示E. 大肠杆菌O157：H7在样品中，而不是大肠杆菌O55：H7。