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公开(公告)号：US20090069238A1
公开(公告)日：2009-03-12
申请号：US12192900
申请日：2008-08-15
申请人： Lance E. Steward , Joseph Francis , Ester Fernandez-Salas , Sanjiv Ghanshani , Marcella A. Gilmore , Shengwen Li , J. Oliver Dolly , Kei Roger Aoki
发明人： Lance E. Steward , Joseph Francis , Ester Fernandez-Salas , Sanjiv Ghanshani , Marcella A. Gilmore , Shengwen Li , J. Oliver Dolly , Kei Roger Aoki
CPC分类号： C07K14/33 , A61K38/00 , C12N9/52 , Y02A50/469
摘要： Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids.
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公开(公告)号：US07495069B2
公开(公告)日：2009-02-24
申请号：US11609189
申请日：2006-12-11
CPC分类号： G01N33/582
摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
摘要翻译： 本发明提供了可用于测定任何梭菌毒素（包括所有血清型的肉毒毒素）以及破伤风毒素的蛋白酶活性的梭菌毒素底物。 本发明的梭菌毒素底物含有供体荧光团; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 和梭菌毒素识别序列，其包括切割位点，其中切割位点插入供体荧光团和受体之间，并且在适当条件下，在供体荧光团和受体之间显示共振能量转移。
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公开(公告)号：US20090042231A1
公开(公告)日：2009-02-12
申请号：US12192798
申请日：2008-10-21
IPC分类号： C12Q1/37
CPC分类号： C12Q1/37 , C07K14/001 , C07K14/435 , G01N33/56911 , G01N2333/33
摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
摘要翻译： 本发明提供了可用于测定任何梭菌毒素（包括所有血清型的肉毒毒素）以及破伤风毒素的蛋白酶活性的梭菌毒素底物。 本发明的梭菌毒素底物含有供体荧光团; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 和梭菌毒素识别序列，其包括切割位点，其中切割位点插入供体荧光团和受体之间，并且在适当条件下，在供体荧光团和受体之间显示共振能量转移。
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公开(公告)号：US20090004225A1
公开(公告)日：2009-01-01
申请号：US12192613
申请日：2008-08-15
CPC分类号： C07K14/33 , A61K38/00 , A61K47/64 , C07K2319/03 , C07K2319/10 , C07K2319/50 , C07K2319/55
摘要： The present invention relates to a compound comprising a toxin linked to a translocator. Non-limiting examples of toxins of the present invention are botulinum toxin, butyricum toxin, tetani toxins and the light chains thereof. In some embodiments, the translocator of the present invention comprises a protein transduction domain.
摘要翻译： 本发明涉及包含与易位素连接的毒素的化合物。 本发明毒素的非限制性实例是肉毒杆菌毒素，丁酸毒素，破伤风毒素及其轻链。 在一些实施方案中，本发明的易位点包含蛋白质转导结构域。
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公开(公告)号：US08124357B2
公开(公告)日：2012-02-28
申请号：US11908438
申请日：2006-04-04
CPC分类号： G01N33/5014 , G01N33/5058 , G01N33/542 , G01N33/56911 , G01N33/94
摘要： Compositions useful for detecting Clostridial toxin activity comprising a cell that comprises a membrane-associated Clostridial toxin substrate comprising a first member of a fluorescence resonance energy transfer pair; and a Clostridial toxin recognition sequence including a cleavage site; and a membrane-associated second member of the FRET pair and methods useful for determining Clostridial toxin activity using such Clostridial toxin substrates.
摘要翻译： 用于检测梭菌毒素活性的组合物，其包含细胞，所述细胞包含与所述膜相关的梭菌毒素底物，所述底物包含荧光共振能量转移对的第一成员; 和包含切割位点的梭菌毒素识别序列; 和FRET对的膜相关的第二个成员以及可用于使用这种梭菌毒素底物测定梭菌毒素活性的方法。
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公开(公告)号：US08071110B2
公开(公告)日：2011-12-06
申请号：US12192546
申请日：2008-08-15
申请人： Lance E. Steward , Joseph Francis , Ester Fernandez-Salas , Sanjiv Ghanshani , Marcella A. Gilmore , Shengwen Li , J. Oliver Dolly , Kei Roger Aoki
发明人： Lance E. Steward , Joseph Francis , Ester Fernandez-Salas , Sanjiv Ghanshani , Marcella A. Gilmore , Shengwen Li , J. Oliver Dolly , Kei Roger Aoki
CPC分类号： C07K14/33 , A61K38/00 , C12N9/52 , Y02A50/469
摘要： Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids.
摘要翻译： 包含可活化的重组神经毒素和由其衍生的多肽的组合物。 本发明还包括编码这种多肽的核酸，以及制备这种多肽和核酸的方法。
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公开(公告)号：US08053209B2
公开(公告)日：2011-11-08
申请号：US12620417
申请日：2009-11-17
CPC分类号： C12Q1/37 , C07K14/001 , C07K14/435 , G01N33/56911 , G01N2333/33
摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
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28.发明授权Modified clostridial toxins with altered targeting capabilities for clostridial toxin target cells 有权对梭菌毒素靶细胞具有改变的靶向能力的改良梭菌毒素公开(公告)号：US08052979B2
公开(公告)日：2011-11-08
申请号：US11817937
申请日：2006-03-14
申请人： Lance E. Steward , Ester Fernandez-Salas , Joseph Francis , Shengwen Li , Marcella A. Gilmore , Kei Roger Aoki
发明人： Lance E. Steward , Ester Fernandez-Salas , Joseph Francis , Shengwen Li , Marcella A. Gilmore , Kei Roger Aoki
CPC分类号： C07K14/33 , C07K2319/035 , C07K2319/33 , C07K2319/50 , C07K2319/55
摘要： The specification discloses modified Clostridial toxins comprising a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and an enhanced Clostridial toxin binding domain; polynucleotide molecules encoding such modified Clostridial toxins; and method of producing such modified Clostridial toxins.
摘要翻译： 该说明书公开了修饰梭菌毒素，其包含梭菌毒素酶促结构域，梭菌毒素易位结构域和增强的梭菌毒素结合结构域; 编码这种经修饰的梭菌毒素的多核苷酸分子; 以及生产这种经修饰的梭菌毒素的方法。
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公开(公告)号：US08048643B2
公开(公告)日：2011-11-01
申请号：US12620388
申请日：2009-11-17
IPC分类号： C12Q1/37 , A61K38/00 , G01N33/533 , C07K7/00 , C07K14/00
CPC分类号： C12Q1/37 , C07K14/001 , C07K14/435 , G01N33/56911 , G01N2333/33
摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
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30.发明授权公开(公告)号：US08021859B2
公开(公告)日：2011-09-20
申请号：US12192934
申请日：2008-08-15
申请人： Lance E. Steward , Ester Fernandez-Salas , Joseph Francis , Shengwen Li , Marcella A. Gilmore , Kei Roger Aoki
发明人： Lance E. Steward , Ester Fernandez-Salas , Joseph Francis , Shengwen Li , Marcella A. Gilmore , Kei Roger Aoki
CPC分类号： C07K14/33
摘要： The specification discloses modified Clostridial toxins comprising a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and an enhanced Clostridial toxin binding domain; polynucleotide molecules encoding such modified Clostridial toxins; and method of producing such modified Clostridial toxins.
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