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    Methods of activating clostridial toxins
    2.
    发明授权
    Methods of activating clostridial toxins 失效
    活化梭菌毒素的方法

    公开(公告)号：US08486422B2

    公开(公告)日：2013-07-16

    申请号：US12669447

    申请日：2008-06-27

    申请人： Marc F. Verhagen ,  Dean G. Stathakis ,  Lance E. Steward

    发明人： Marc F. Verhagen ,  Dean G. Stathakis ,  Lance E. Steward

    IPC分类号： A61K39/08

    CPC分类号： C07K14/33 ,  C07K2319/50 ,  C12P21/02

    摘要： The specification discloses modified Clostridial toxins comprising an exogenous Clostridial toxin di-chain loop protease cleavage site located within the di-chain loop region; polynucleotide molecules encoding such modified Clostridial toxins; method of producing such modified Clostridial toxins, method of activating such modified Clostridial toxins and methods of activating recombinantly-expressed Clostridial toxins.

    摘要翻译： 该说明书公开了修饰的梭菌毒素，其包含位于二链环区内的外源性梭菌毒素二链环蛋白酶切割位点; 编码这种经修饰的梭菌毒素的多核苷酸分子; 生产这种经修饰的梭菌毒素的方法，活化这种经修饰的梭菌毒素的方法和活化重组表达梭​​菌毒素的方法。

    Degradable clostridial toxins
    8.
    发明授权
    Degradable clostridial toxins 有权
    可降解梭菌毒素

    公开(公告)号：US07998489B2

    公开(公告)日：2011-08-16

    申请号：US12192905

    申请日：2008-08-15

    申请人： Lance E. Steward ,  Ester G. Fernandez-Salas ,  Marcella A. Gilmore ,  Joseph Francis ,  Shengwen Li ,  Kei Roger Aoki

    发明人： Lance E. Steward ,  Ester G. Fernandez-Salas ,  Marcella A. Gilmore ,  Joseph Francis ,  Shengwen Li ,  Kei Roger Aoki

    IPC分类号： C07K14/00 ,  C07K14/33 ,  C07H21/04

    CPC分类号： C07K14/33 ,  C07K2319/33 ,  C07K2319/55

    摘要： The specification discloses modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain; polynucleotide molecules encoding modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain; and method of producing modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain.

    摘要翻译： 该说明书公开了修饰的梭菌毒素，其包含PAR配体结构域，梭菌毒素酶促结构域，梭菌毒素易位结构域和梭菌毒素结合结构域; 编码经修饰的梭菌毒素的多核苷酸分子，其包含PAR配体结构域，梭菌毒素酶促结构域，梭菌毒素易位结构域和梭菌毒素结合结构域; 以及产生包含PAR配体结构域，梭菌毒素酶促结构域，梭菌毒素易位结构域和梭菌毒素结合结构域的修饰梭菌毒素的方法。

    FRET PROTEASE ASSAYS FOR CLOSTRIDIAL TOXINS
    9.
    发明申请
    FRET PROTEASE ASSAYS FOR CLOSTRIDIAL TOXINS 有权
    用于封闭性毒素的FRET蛋白质测定

    公开(公告)号：US20100151494A1

    公开(公告)日：2010-06-17

    申请号：US12620400

    申请日：2009-11-17

    申请人： Lance E. Steward ,  Ester Fernandez-Salas ,  Kei Roger Aoki

    发明人： Lance E. Steward ,  Ester Fernandez-Salas ,  Kei Roger Aoki

    IPC分类号： G01N33/573 ,  C07K7/06 ,  C07K14/00 ,  C07K7/08

    CPC分类号： C12Q1/37 ,  C07K14/001 ,  C07K14/435 ,  G01N33/56911 ,  G01N2333/33

    摘要： The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.

    摘要翻译： 本发明提供了可用于测定任何梭菌毒素（包括所有血清型的肉毒毒素）以及破伤风毒素的蛋白酶活性的梭菌毒素底物。 本发明的梭菌毒素底物含有供体荧光团; 具有与供体荧光团的发射光谱重叠的吸收光谱的受体; 和梭菌毒素识别序列，其包括切割位点，其中切割位点插入供体荧光团和受体之间，并且在适当条件下，在供体荧光团和受体之间显示共振能量转移。

    Leucine-based motif and clostridial neurotoxins
    10.
    发明授权
    Leucine-based motif and clostridial neurotoxins 有权

    公开(公告)号：US07691974B2

    公开(公告)日：2010-04-06

    申请号：US12054667

    申请日：2008-03-25

    申请人： Lance E. Steward ,  Todd M. Herrington ,  Kei Roger Aoki

    发明人： Lance E. Steward ,  Todd M. Herrington ,  Kei Roger Aoki

    IPC分类号： C07K14/33

    CPC分类号： C07K14/33 ,  A61K38/00 ,  Y10S530/825

    摘要： Modified neurotoxin comprising neurotoxin including structural modification, wherein the structural modification alters the biological persistence, preferably the biological half-life, of the modified neurotoxin relative to an identical neurotoxin without the structural modification. The structural modification includes addition or deletion of a leucine-based motif or parts thereof. In one embodiment, methods of making the modified neurotoxin include using recombinant techniques. In another embodiment, methods of using the modified neurotoxin to treat biological disorders include treating autonomic disorders, neuromuscular disorders or pains.