公开(公告)号：US06020186A

公开(公告)日：2000-02-01

申请号：US442406

申请日：1995-05-16

Applicant: Karsten Henco ,  Metin Colpan ,  Petra Feuser

Inventor： Karsten Henco ,  Metin Colpan ,  Petra Feuser

IPC: C12N15/10 ,  C12Q1/68 ,  C12N3/00

CPC classification number: C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

Abstract: A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps:a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed;b) the cells are lysed;c) the nucleic acids are fixated on the matrix surface, and subsequentlyd) are eluted.

Abstract translation: 通过以下步骤裂解完整细胞和从裂解的细胞中除去出现的核酸来分离核酸的装置和方法：a）将细胞固定在多孔基质中，其中基质空隙的大小在 待裂解的细胞类型; b）细胞裂解; c）将核酸固定在基质表面上，随后d）被洗脱。

公开(公告)号：US5464541A

公开(公告)日：1995-11-07

申请号：US153463

申请日：1993-11-17

Applicant: James E. Aysta ,  Metin Colpan

Inventor： James E. Aysta ,  Metin Colpan

IPC: B01D15/00 ,  B01D24/00 ,  B01D29/01 ,  B01D61/18 ,  B01L3/00 ,  B01L99/00 ,  C12M1/00 ,  C12Q1/68 ,  B01D29/00

CPC classification number: B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L3/50855 ,  Y10T436/25375 ,  Y10T436/255

Abstract: A device (10) for separating liquid samples has a sample container (12), bottom wall (20) of which has an outlet opening (22) provided therein. The outlet opening (22) is joined by an outlet spout (24) extending in the axial direction of the sample container (12). On bottom wall (20) there is a separation layer (28). Below sample container (12), a collecting container (32) is arranged abutting sample container (12) such that an exchange of air between the interior of the collecting chamber (32) and the environment is possible, yet an escape of liquid is largely inhibited. The contact surface between collecting chamber (32) and sample container (12) and end (26) of the outlet spout (24) through which the liquid is discharged, are axially spaced apart.

Abstract translation: 用于分离液体样品的装置（10）具有样品容器（12），其底壁（20）具有设置在其中的出口（22）。 出口开口（22）通过在样品容器（12）的轴向方向上延伸的出口喷口（24）连接。 在底壁（20）上有分离层（28）。 在样本容器（12）的下面，收集容器（32）设置成邻接样本容器（12），使得可以在收集室（32）的内部和环境之间交换空气，但是液体逸出很大程度上 抑制。 收集室（32）和样品容器（12）之间的接触表面以及排出液体的出口喷口（24）的端部（26）轴向间隔开。