公开(公告)号：US20220256863A1

公开(公告)日：2022-08-18

申请号：US17556428

申请日：2021-12-20

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Matthew S. Marengo ,  Jason Meyer ,  Jason S. Milligan ,  Brian E. Weiner

IPC: A01N63/50 ,  C07K14/325 ,  C12Q1/6895 ,  C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4029, TIC4029_1, and TIC4029_8. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding pesticidal proteins of the TIC4029 class. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the TIC4029 class in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4029, TIC4029_1, and TIC4029_8 pesticidal proteins are also provided.

公开(公告)号：US11198888B2

公开(公告)日：2021-12-14

申请号：US16684007

申请日：2019-11-14

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/20

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US11130964B2

公开(公告)日：2021-09-28

申请号：US16205426

申请日：2018-11-30

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/50

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US20200370066A1

公开(公告)日：2020-11-26

申请号：US16895921

申请日：2020-06-08

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Jason S. Milligan ,  James K. Roberts ,  Yong Yin

IPC: C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

公开(公告)号：US10703782B2

公开(公告)日：2020-07-07

申请号：US15868676

申请日：2018-01-11

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  David J. Bowen ,  Catherine A. Chay ,  David J. Chi ,  William P. Clinton ,  Crystal L. Dart ,  Leigh English ,  Stanislaw Flasinski ,  Victor M. Guzov ,  Kevin A. Jarrell ,  Uma R. Kesanapalli ,  Thomas M. Malvar ,  Robert M. McCarroll ,  Jason S. Milligan ,  Jay P. Morgenstern ,  Deborah G. Rucker ,  Sara A. Salvador ,  Temple F. Smith ,  Carlos E. Soto ,  Collin M. Stultz ,  Brian M. Turczyk ,  Ty T. Vaughn ,  Moritz W. F. Von Rechenberg

IPC: C07K14/325 ,  C12N15/82

Abstract: Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

公开(公告)号：US10662439B2

公开(公告)日：2020-05-26

申请号：US14945140

申请日：2015-11-18

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/20

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.