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    Variants of Glycoside Hydrolases
    26.
    发明申请
    Variants of Glycoside Hydrolases 审中-公开
    糖苷水解酶的变体

    公开(公告)号：US20150056664A1

    公开(公告)日：2015-02-26

    申请号：US14475639

    申请日：2014-09-03

    Applicant: NOVOZYMES INC.

    Inventor： Sarah Teter ,  Connie Ward ,  Joel Cherry ,  Aubrey Jones ,  Paul Harris ,  Jung Yi

    IPC: C12N9/42 ,  C12P19/02 ,  C11D3/386 ,  C12P19/14

    CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

    Abstract: The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    Abstract translation: 本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。 本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Methods For Increasing Homologous Recombination Of A Nucleic Acid Sequence
    27.
    发明申请
    Methods For Increasing Homologous Recombination Of A Nucleic Acid Sequence 审中-公开
    增加核酸序列同源重组的方法

    公开(公告)号：US20150056652A1

    公开(公告)日：2015-02-26

    申请号：US14536048

    申请日：2014-11-07

    Applicant: NOVOZYMES, INC.

    Inventor： Paul Harris ,  Howard Brody

    IPC: C12P21/00

    CPC classification number: C12P21/00 ,  C12N15/80 ,  C12N15/902

    Abstract: The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.

    Abstract translation: 本发明涉及用于增加引入宿主细胞的核酸序列的同源重组的方法，其包括：（a）向丝状真菌宿主细胞群体中引入编码重组蛋白和第二核酸序列的第一核酸序列 包括与丝状真菌宿主细胞的基因组同源的一个或多个区域，其中（i）重组蛋白促进一个或多个区域与宿主基因组中的相应同源区域的重组，以掺入第二核酸序列 通过同源重组，和（ii）与没有第一核酸序列的相同群体相比，包含所述群体中掺入的第二核酸序列的宿主细胞的数量增加至少20％; （b）并从群体中分离包含掺入的第二核酸序列的丝状真菌细胞。

    Methods of increasing the cellulolytic enhancing activity of a polypeptide
    29.
    发明授权
    Methods of increasing the cellulolytic enhancing activity of a polypeptide 有权

    公开(公告)号：US10689636B2

    公开(公告)日：2020-06-23

    申请号：US15901567

    申请日：2018-02-21

    Applicant: Novozymes, Inc.

    Inventor： Keith McFarland ,  Paul Harris

    IPC: C12N9/42 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14

    Abstract: The present invention relates to methods of increasing the activity of a polypeptide having cellulolytic enhancing activity, comprising: adding a soluble activating divalent metal cation to a composition comprising the polypeptide having cellulolytic enhancing activity, wherein the presence of the soluble activating divalent metal cation and the polypeptide having cellulolytic enhancing activity increases degradation or conversion of a cellulose-containing material by a cellulolytic enzyme composition compared to the polypeptide having cellulolytic enhancing activity without the soluble activating divalent metal cation. The present invention also relates to compositions, methods for degrading or converting a cellulose-containing material, and methods for producing a fermentation product.

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