公开(公告)号：US06770892B2

公开(公告)日：2004-08-03

申请号：US10086743

申请日：2002-02-28

申请人： John F. Corson ,  Stanley P. Woods ,  Russell A. Parker ,  Xiangyang Zhou ,  Bo U. Curry

发明人： John F. Corson ,  Stanley P. Woods ,  Russell A. Parker ,  Xiangyang Zhou ,  Bo U. Curry

IPC分类号： G01T2164

CPC分类号： G01N21/6452 ,  G01N21/6458 ,  G01N2021/6463

摘要： An automated method and system for determining an optimal focus distance for scanning a molecular array scanner. Blocks of rows of a reference array are automatically scanned at successively greater distances of the stage from a light gathering medium, such as an optical fiber, or z-positions, to produce data providing a functional relationship between z-position and measured signal intensities. The data is then processed by a peak-height-based, or window-based, focus-finding routine that selects an optimal focus-distance for data scans.

摘要翻译： 一种用于确定用于扫描分子阵列扫描仪的最佳聚焦距离的自动化方法和系统。 参考阵列的行的块自动地从阶段相对较远的距离光收集介质（诸如光纤或z位置）扫描，以产生提供z位置和测量的信号强度之间的功能关系的数据。 然后通过基于峰值高度或基于窗口的聚焦寻找程序处理数据，该程序为数据扫描选择最佳聚焦距离。

公开(公告)号：US5577178A

公开(公告)日：1996-11-19

申请号：US566602

申请日：1995-12-04

申请人： Bo U. Curry ,  David R. Smith

发明人： Bo U. Curry ,  David R. Smith

IPC分类号： G06F15/18 ,  G06N3/00 ,  G06T1/40 ,  G09G5/02 ,  H04N1/46 ,  H04N1/60 ,  G06F15/00

CPC分类号： H04N1/603

摘要： A neural network for converting pixels represented in one color representation to pixels of a second color representation. One realization of the neural network utilizes an input layer that includes either a summing node or a product generating node. The neural network may be implemented in analog circuitry or as a simulation on a general purpose data processor. The analog circuit implementation requires a relatively small number of nodes and is less expensive than implementations based on general purpose data processors.

摘要翻译： 一种用于将在一种颜色表示中表示的像素转换为第二颜色表示的像素的神经网络。 神经网络的一个实现使用包括求和节点或产品生成节点的输入层。 神经网络可以在模拟电路中实现，或者作为通用数据处理器上的模拟来实现。 模拟电路实现需要相对较少数量的节点，并且比基于通用数据处理器的实现便宜。

公开(公告)号：US08765642B2

公开(公告)日：2014-07-01

申请号：US13448985

申请日：2012-04-17

申请人： Bo U. Curry

发明人： Bo U. Curry

IPC分类号： C40B40/00

CPC分类号： C12Q1/6841 ,  C12Q1/689 ,  C12Q2537/143 ,  C12Q2543/10 ,  C12Q2563/107 ,  C12Q2565/601

摘要： Provided herein is a set of reagents comprising: a plurality of at least three probe libraries, wherein each library of the plurality comprises one or more probe sets that are each specific for a target; and at least one of the libraries comprises a probe set that is present in another of the libraries. The plurality of libraries can be hybridized to spatially separated targets, simultaneously or sequentially. The identity of a spatially separated target can be determined by identifying which combination of the multiple libraries hybridize thereto.

摘要翻译： 本文提供了一组试剂，其包括：多个至少三个探针文库，其中多个文库中的每个文库包含一个或多个探针组，每个探针组各自针对靶标; 并且至少一个库包括存在于另一个文库中的探针组。 多个文库可以与空间分离的目标同时或顺序地杂交。 可以通过鉴定多个文库与其杂交的哪个组合来确定空间分离的靶的身份。

公开(公告)号：US20080164424A1

公开(公告)日：2008-07-10

申请号：US11555185

申请日：2007-01-10

申请人： Joel Myerson ,  Bo U. Curry

发明人： Joel Myerson ,  Bo U. Curry

IPC分类号： G21G5/00

CPC分类号： G01N33/54393

摘要： The invention relates to novel methods for reducing background fluorescence of arrays. In particular embodiments, the invention provides a method of treating an array by exposing the array to light wherein the array receives at least a specified dosage of light. In various embodiments the invention also provides a device for exposing an array to light, wherein the device includes: (a) a light source capable of producing light; and (b) an array holder configured to hold the array, the array holder disposed to permit the array to receive the light from the light source.

摘要翻译： 本发明涉及减少阵列背景荧光的新方法。 在具体实施方案中，本发明提供了一种通过将阵列暴露于光来处理阵列的方法，其中阵列接收至少一定量的光。 在各种实施例中，本发明还提供了一种用于将阵列暴露于光的装置，其中该装置包括：（a）能产生光的光源; 和（b）配置成保持阵列的阵列保持器，所述阵列保持器设置成允许阵列接收来自光源的光。

公开(公告)号：US07221785B2

公开(公告)日：2007-05-22

申请号：US10153394

申请日：2002-05-21

申请人： Bo U. Curry ,  Herbert F. Cattell ,  Srinka Ghosh

发明人： Bo U. Curry ,  Herbert F. Cattell ,  Srinka Ghosh

IPC分类号： G06K9/00 ,  G06K9/40 ,  G01N33/48

CPC分类号： G06K9/00 ,  G06T7/11 ,  G06T7/194 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30072

摘要： A method and system for estimating the background signal over an arbitrarily-sized region of a scanned image of a molecular array, including a background region surrounding the ROI corresponding to the feature. A bit mask is generated, based on a molecular-array, feature-based data set that includes pixel-based intensities and a list of features, including feature coordinates and feature ROI radii, to indicate those pixels in the scanned image of the molecular array corresponding to background, and those pixels in the scanned image of the molecular array corresponding to features and ROIs. An integrated intensity for a background region of arbitrary size and shape can be efficiently determined by selecting pixels within the background region that are indicated to be background pixels in the bit mask. By selecting background anuli of sufficient size to overlap with the background anuli of neighboring features, a relatively continuous function of background-signal-verusus-position can be obtained across the surface of a molecular array in order to examine non-local, background-signal-related phenomena.

摘要翻译： 一种用于在分子阵列的扫描图像的任意大小的区域上估计背景信号的方法和系统，包括与特征对应的ROI的背景区域。 基于分子阵列生成位掩模，基于特征的数据集，其包括基于像素的强度和包括特征坐标和特征ROI半径的特征列表，以指示分子阵列的扫描图像中的那些像素 对应于背景，并且分子阵列的扫描图像中的像素对应于特征和ROI。 可以通过选择背景区域中被指示为位掩码中的背景像素的像素来有效地确定用于任意尺寸和形状的背景区域的积分强度。 通过选择具有足够大小的背景与相邻特征的背景重叠的背景，可以跨分子阵列的表面获得背景信号 - 维基位置的相对连续的函数，以便检查非局部背景信号 相关现象。

公开(公告)号：US07067783B2

公开(公告)日：2006-06-27

申请号：US10261359

申请日：2002-09-30

申请人： Bo U. Curry ,  Andreas N. Dorsel ,  Jayati Ghosh ,  Kenneth L. Staton

发明人： Bo U. Curry ,  Andreas N. Dorsel ,  Jayati Ghosh ,  Kenneth L. Staton

IPC分类号： G02B7/04

CPC分类号： G02B7/36 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6421

摘要： Automated methods and systems for determining an in-focus-distance for a position on the surface of a molecular array substrate using a molecular array scanner are provided. A signal from a first position of an array substrate is detected and noise is filtered out of the detected signal using a symmetrical filter to produce an in-focus-distance. In one embodiment, the in-focus-distance is utilized as an estimated in-focus-distance at a second position of the array substrate. The method finds use in maintaining the focus of a light source while scanning the array by the scanner. Also provided are methods of assaying a sample using the methods and systems of the invention, and kits for performing the invention. The subject invention finds use in a variety of different applications, including both genomics and proteomics applications.

摘要翻译： 提供了使用分子阵列扫描仪来确定分子阵列衬底表面上的位置的对焦距离的自动化方法和系统。 检测来自阵列基板的第一位置的信号，并使用对称滤波器将噪声从检测信号中滤出，以产生对焦距离。 在一个实施例中，将聚焦距离用作阵列基板的第二位置处的估计的对焦距离。 该方法用于在扫描仪扫描阵列时保持光源的焦点。 还提供了使用本发明的方法和系统测定样品的方法以及用于实施本发明的试剂盒。 本发明可用于各种不同的应用，包括基因组学和蛋白质组学应用。

公开(公告)号：US07057185B2

公开(公告)日：2006-06-06

申请号：US10910552

申请日：2004-08-02

申请人： Bo U. Curry ,  Andreas N. Dorsel ,  Kyle J. Schleifer ,  Debra A. Sillman

发明人： Bo U. Curry ,  Andreas N. Dorsel ,  Kyle J. Schleifer ,  Debra A. Sillman

IPC分类号： G01J1/20

CPC分类号： G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6484

摘要： A maximum sensitivity optical scanning system is disclosed. It finds use in a variety of applications, including the reading of biopolymeric arrays. It operates by scanning sample at a setting selected to result in signal saturation for some, but not all available data. Subsequent scans of the same area are taken at lower sensitivity settings (in terms of detector gain and/or excitation light source gain or attenuation) and data from at least the previously saturated regions is obtained. If system sensitivity is set too low to produce useful results, optional features may adjust sensitivity upward and follow with an increased sensitivity scan as a remedial measure. Full signal sensitivity is better preserved as most needed in taking data for the weakest signals first with the high-level scan. Data for sample producing stronger signals that can better tolerate photobleaching is then taken in one way or another.

公开(公告)号：US5398539A

公开(公告)日：1995-03-21

申请号：US101322

申请日：1993-08-02

申请人： Gary B. Gordon ,  Bo U. Curry

发明人： Gary B. Gordon ,  Bo U. Curry

IPC分类号： G01N30/46 ,  G01N30/86 ,  G01N30/02 ,  B01D15/08

CPC分类号： G01N30/8624 ,  G01N30/463 ,  G01N30/466 ,  G01N30/8631

摘要： In a correlated two-dimensional gas chromatography system, it is necessary to "pair" peaks from one chromatogram with respective peaks of another chromatogram. Both peaks of a pair should correspond to the same sample component. The present invention provides for confirmation/disconfirmation of pairs that may be speculative or arbitrary. A hybrid chromatographic column is designed so that the retention time of a sample component is the average of the retention times of that component in the two independent columns. Thus, a peak location in the hybrid chromatogram can be calculated for each pair of peaks. The absence of a peak at that location or the inconsistency of the area of a peak at that location disconfirms the pairing. The invention also provides for higher dimensional systems and for other separation technologies.

摘要翻译： 在相关的二维气相色谱系统中，必须将来自一个色谱图的峰“配对”与另一个色谱图的相应峰。 一对的两个峰应对应于相同的样品成分。 本发明提供了可能是推测性或任意性的对的确认/不确认。 设计混合色谱柱，使得样品组分的保留时间是该组分在两个独立色谱柱中的保留时间的平均值。 因此，可以为每对峰值计算混合色谱图中的峰位置。 在该地点没有峰值或该地点的峰值区域的不一致性证实了配对。 本发明还提供了更高维度的系统和其他分离技术。