公开(公告)号：US12102941B2

公开(公告)日：2024-10-01

申请号：US17383117

申请日：2021-07-22

申请人： Tata Consultancy Services Limited

发明人： Venkataramana Runkana ,  Venkata Sudheendra Buddhiraju ,  Aditya Pareek ,  Vishnu Swaroopji Masampally ,  Karundev Premraj

IPC分类号： B01D15/38 ,  B01D15/18 ,  C07K1/16 ,  C07K1/36 ,  G01N30/46

CPC分类号： B01D15/3809 ,  B01D15/1885 ,  C07K1/16 ,  C07K1/36 ,  G01N30/466

摘要： The disclosure generally relates to methods and systems for determining multi-column chromatography process configuration for capturing antibodies. Conventional approaches for design of MCC configuration are limited to rule based, either driven by UV spectroscopic measurements or by performing number of experiments, which involves a lot of material costs and time utilization. The present disclosure solves the technical problem of identifying the operational conditions that optimized the MCC process and the MCC configuration. A multi-objective optimization function defined with one or more decision variables associated with the operating conditions is considered to determine the optimal MCC configuration, while satisfying purification goals. The one or more key performance measures of the MCC process comprises a productivity, a capacity utilization, a product yield, and a product purity. The significant amount of time and the material cost invested for designing the optimum MCC configuration is decreased by the present disclosure.

公开(公告)号：US12078619B2

公开(公告)日：2024-09-03

申请号：US17288478

申请日：2018-10-26

申请人： Agilent Technologies, Inc.

发明人： Thomas Ortmann

IPC分类号： G01N30/20 ,  B01D15/18 ,  G01N30/22 ,  G01N30/46 ,  G01N30/60

CPC分类号： G01N30/20 ,  B01D15/1885 ,  G01N30/22 ,  G01N30/466 ,  G01N30/6043 ,  G01N2030/202 ,  G01N2030/207

摘要： An injector, for injecting a fluidic sample in at least one selected one of a first sample separation apparatus and a second sample separation apparatus, includes a valve arrangement fluidically connectable to the first sample separation apparatus and the second sample separation apparatus, a sample accommodation volume for accommodating the fluidic sample, and a control unit configured for controlling the valve arrangement so that fluidic sample in the sample accommodation volume is selectively injectable into the selected first sample separation apparatus and/or second sample separation apparatus.

公开(公告)号：US11933769B2

公开(公告)日：2024-03-19

申请号：US17051701

申请日：2019-04-30

申请人： Agilent Technologies, Inc.

发明人： Dwight Robert Stoll ,  Klaus Witt ,  Konstantin Shoykhet

IPC分类号： G01N30/32 ,  G01N30/46

CPC分类号： G01N30/32 ,  G01N30/463 ,  G01N30/466 ,  G01N30/468

摘要： A multidimensional sample separation apparatus includes a first separation dimension for separating a fluidic sample, a second separation dimension for further separating the fluidic sample, a switching mechanism, and a control unit. The first separation dimension includes a first fluid drive unit and a first sample separation unit. The second separation dimension includes a second fluid drive unit for driving the separated fluidic sample, and second and third sample separation units each configured for further separating the separated fluidic sample. The switching mechanism is configured for selectively switching an outlet of the first separation dimension in fluid communication with a selected one of the second sample separation unit and/or the third sample separation unit. The control unit is configured for controlling a pressure at inlets of the second sample separation unit and the third sample separation unit to be substantially the same at least at the time of the switching.

公开(公告)号：US11867673B2

公开(公告)日：2024-01-09

申请号：US17436599

申请日：2020-03-20

申请人： INDIAN INSTITUTE OF TECHNOLOGY DELHI

发明人： Anurag Singh Rathore ,  Garima Thakur ,  Vishwanath Hebbi

IPC分类号： G01N21/359 ,  G01N21/3577 ,  G01N21/25 ,  G01N33/68 ,  G01N30/86 ,  G01N30/46 ,  G01N30/74 ,  G01N30/88

CPC分类号： G01N30/8658 ,  G01N30/466 ,  G01N2030/746 ,  G01N2030/889 ,  G01N2030/8831

摘要： The present invention relates to method of using spectroscopy for real time measuring of concentration of desired product and using measured data for monitoring and control of chromatography. It develops a method and system for measuring real-time concentration of clarified harvest and that of flow through of loading step of the chromatography and using measured data for determining breakthrough in real-time. The two modes of operation are used viz. first mode (Part A) uses a single near infrared spectroscopy (NIR) flow cell prior to the continuous chromatography column to ensure optimal loading in each cycle based on dynamic binding capacity studies carried out previously with the desired Protein A resin and second mode (Part B) uses two near infrared spectroscopy (NIR) flow cells, one before and one after the column, to detect the breakthrough curve (from 1% breakthrough onwards).

公开(公告)号：US20190212308A1

公开(公告)日：2019-07-11

申请号：US16099234

申请日：2016-06-30

申请人： Shimadzu Corporation

发明人： Kiyonori KOGA ,  Yuki KOMORI

IPC分类号： G01N30/32 ,  G01N30/46

CPC分类号： G01N30/32 ,  G01N30/466 ,  G01N2030/025 ,  G01N2030/328

摘要： A flow controller includes a channel assembly, a pressure sensor, and a pressure control valve. The channel assembly includes a laminated substrate comprised of a plurality of substrates laminated, the laminated substrate having a surface on which a gas inlet and a gas outlet are formed, and having, inside the laminated substrate, a plurality of resistance channels not communicating with one another inside the laminated substrate as internal channels. In the flow controller, the gas inlet and the gas outlet communicate to each other to form a flow rate control channel. The pressure sensor and the pressure control valve are provided on the flow rate control channel. At least one of the resistance channels which is selected to have a desired channel resistance of the flow rate control channel is connected to the flow rate control channel as part of the flow rate control channel.

公开(公告)号：US20170056877A1

公开(公告)日：2017-03-02

申请号：US15292093

申请日：2016-10-12

申请人： GE Healthcare BioProcess R&D AB

发明人： Karol Lacki

IPC分类号： B01L3/00 ,  G01N30/60 ,  G01N30/46

CPC分类号： B01L3/50255 ,  B01L3/5085 ,  B01L2300/069 ,  G01N30/466 ,  G01N30/6043 ,  G01N30/8658 ,  Y10T436/25

摘要： A micro titre plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.

摘要翻译： 包含多个填充有分离基质的孔的微量滴定板。 根据本发明，分离基质的体积在至少一些孔之间变化。

公开(公告)号：US20150040992A1

公开(公告)日：2015-02-12

申请号：US14381971

申请日：2013-03-07

申请人： Waters Technologies Corporation

发明人： Joshua A. Shreve ,  Kurt Joudrey ,  John Angelosanto

IPC分类号： F17C1/00

CPC分类号： G01N30/02 ,  B01D15/163 ,  B01D15/40 ,  F17C1/00 ,  G01N30/32 ,  G01N30/36 ,  G01N30/466 ,  G01N2030/027 ,  Y02P20/544 ,  Y10T137/0396 ,  Y10T137/86027 ,  Y10T137/86035 ,  Y10T137/87314 ,  Y10T137/87507 ,  Y10T137/87917 ,  Y10T137/87925

摘要： Exemplary embodiments are directed to devices, methods and systems capable of pressurization, generally involving a flow system that includes a pressurized reservoir, at least one pump including a pump control valve, an outlet port, a shut-off valve and a vent valve. The flow system is configured to be pressurized. The shut-off valve is disposed between the pressurized reservoir and the at least one pump. The vent valve is disposed between the at least one pump and the outlet port. The shut-off valve, the vent valve and the pump control valve of the at least one pump are configured to actuate in a coordinated manner to control a pressurization of the flow system. Exemplary embodiments are further directed to devices, methods and systems for column switching, generally including at least a first column, a second column and a column switching valve.

摘要翻译： 示例性实施例涉及能够加压的装置，方法和系统，通常包括包括加压储存器的流动系统，至少一个泵，包括泵控制阀，出口，截止阀和排气阀。 流动系统构造成加压。 截止阀设置在加压容器和至少一个泵之间。 排气阀设置在至少一个泵和出口之间。 至少一个泵的截止阀，排气阀和泵控制阀被构造成以协调的方式致动以控制流动系统的加压。 示例性实施例进一步涉及用于列切换的装置，方法和系统，通常包括至少第一列，第二列和列切换阀。

公开(公告)号：US20150019141A1

公开(公告)日：2015-01-15

申请号：US14328926

申请日：2014-07-11

申请人： SHIMADZU CORPORATION

发明人： Hiroshi OHASHI

IPC分类号： G01N30/86 ,  G01N30/02 ,  B01D15/08

CPC分类号： G01N30/8658 ,  B01D15/08 ,  G01N30/34 ,  G01N30/466

摘要： Provided is a liquid chromatograph control system for controlling an operation of a liquid chromatograph according to a method file containing a plurality of analysis parameters representing configuration items which determine operational conditions of the liquid chromatograph, including: a) a display section for displaying, for each of the analysis parameters, selectable values of the analysis parameter on a condition-setting screen; b) a grouping section for allowing a user to prepare grouping information for the values of the analysis parameter displayed on the condition-setting screen, the grouping information determining whether or not one value of one analysis parameter and one value of another analysis parameter can be included in one group; and c) a file-creating section for extracting, from the values of the analysis parameters, such values that can be included in one group according to the grouping information, and for creating a method file containing the extracted values.

摘要翻译： 提供了一种液相色谱控制系统，用于根据包含多个分析参数的方法文件来控制液相色谱仪的操作，所述多个分析参数表示确定液相色谱仪的操作条件的配置项，包括：a）显示部分，用于显示每个 的分析参数，条件设置屏幕上分析参数的可选值; b）分组部分，用于允许用户为条件设置屏幕上显示的分析参数的值准备分组信息，分组信息确定一个分析参数的一个值和另一分析参数的一个值是否可以 包括在一个组中; 以及c）文件创建部分，用于从分析参数的值中提取根据分组信息可以包括在一个组中的这些值，以及用于创建包含所提取的值的方法文件。

公开(公告)号：US08865075B2

公开(公告)日：2014-10-21

申请号：US13284087

申请日：2011-10-28

申请人： Norberto A. Guzman

发明人： Norberto A. Guzman

IPC分类号： G01N21/00 ,  G01N33/543 ,  B01L3/00 ,  G01N33/53 ,  G01N27/447 ,  B01L9/00 ,  G01N30/74 ,  G01N30/46 ,  G01N30/32

CPC分类号： G01N27/44743 ,  B01L3/50 ,  B01L3/502 ,  B01L3/5025 ,  B01L9/527 ,  B01L2200/026 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/021 ,  B01L2300/0627 ,  B01L2300/0809 ,  B01L2300/0861 ,  B01L2400/0415 ,  B01L2400/06 ,  B01L2400/082 ,  G01N30/466 ,  G01N30/74 ,  G01N33/53 ,  G01N33/543 ,  G01N2030/326

摘要： The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as a modular, multi-task immunoaffinity device secured to a peripheral box and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. In one embodiment, two devices are coupled in-tandem to perform separately and sequentially microreactions and concentrations of proteins. In this embodiment, biological samples containing proteins can be subjected to proteolytic processing in the first device, and the resulting peptides subjected to selective purification and concentration in the second device.

摘要翻译： 本发明涉及用于捕获以简单或复杂基质中高或低浓度存在的一种或多种分析物的免疫亲和装置。 该设备被设计为固定到外围盒并连接到毛细管电泳或液相色谱的模块化多任务免疫亲和装置，用于分离，富集，分离和鉴定聚合物大分子，主要是蛋白质生物标志物。 在一个实施方案中，两个装置串联耦合以执行分开且顺序的微反应和蛋白质浓度。 在本实施例中，含有蛋白质的生物样品可以在第一装置中进行蛋白水解处理，所得到的肽在第二装置中进行选择性纯化和浓缩。

公开(公告)号：US20140298990A1

公开(公告)日：2014-10-09

申请号：US14357157

申请日：2012-11-08

申请人： THE REGENTS OF THE UNIVERSITY OF MICHIGAN

发明人： Xudong Fan ,  Yogesh B. Gianchandani ,  Jing Liu

IPC分类号： G01N30/46

CPC分类号： G01N30/461 ,  G01N30/46 ,  G01N30/463 ,  G01N30/465 ,  G01N30/466 ,  G01N30/468 ,  G01N30/6095 ,  G01N30/72

摘要： The present disclosure provides adaptive methods for gas chromatography analysis of a gas sample comprising one or more target analytes (such as a micro-gas chromatography) and adaptive gas chromatography devices for carrying out such analytical methods. Broadly, the system can regulate flow into a downstream chromatographic column by detecting one or more upstream conditions. For example, one adaptive chromatography device comprises a first column, a modulator component, and a second column. A first detector or sensor detects the presence of target analytes upstream from the second column, while a second detector detects the presence of target analytes eluted from the second column. The modulator component assembly is responsive to an output generated by the first detector and adaptively regulates fluid flow into the second column. Such adaptive chromatography (micro-GC) systems have higher separation speed, better analyte identification capability, and far greater energy savings.

摘要翻译： 本公开提供了用于气体样品的气相色谱分析的自适应方法，所述气体样品包含一种或多种靶分析物（例如微气相色谱）和用于进行这种分析方法的适应性气相色谱装置。 通常，系统可以通过检测一个或多个上游条件来调节流入下游色谱柱的流量。 例如，一个自适应色谱装置包括第一列，调制器组件和第二列。 第一检测器或传感器检测来自第二列上游的目标分析物的存在，而第二检测器检测从第二列洗脱的目标分析物的存在。 调制器部件组件响应于由第一检测器产生的输出并且自适应地调节流入第二列的流体流。 这种自适应色谱（micro-GC）系统具有更高的分离速度，更好的分析物识别能力和更大的能量节省。