公开(公告)号：US5935810A

公开(公告)日：1999-08-10

申请号：US347563

申请日：1994-11-30

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca ,  Margherita Maffei ,  Jeffrey L. Halaas ,  Ketan Gajiwala ,  Stephen K. Burley

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca ,  Margherita Maffei ,  Jeffrey L. Halaas ,  Ketan Gajiwala ,  Stephen K. Burley

IPC分类号： A61K8/64 ,  A61K38/00 ,  A61K38/17 ,  A61K48/00 ,  A61Q19/06 ,  C07K14/47 ,  C07K14/575 ,  C07K16/18 ,  C12N1/19 ,  C12N1/21 ,  C12N5/02 ,  C12N5/10 ,  C12N5/16 ,  C12N15/12 ,  C12N15/16 ,  C12Q1/68 ,  G01N33/53 ,  C12N1/15

CPC分类号： C07K14/5759 ,  A61K8/64 ,  A61Q19/06 ,  C07K16/18 ,  C12Q1/6883 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of weight, and to the diagnostic and therapeutic uses to which such modulators may be put. In its broadest aspect, the present invention relates to the elucidation and discovery of nucleotide sequences, and proteins putatively expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian body weight. The nucleotide sequences in object represent the genes corresponding to the murine and human ob gene, that have been postulated to play a critical role in the regulation of body weight and adiposity. Preliminary data, presented herein, suggests that the polypeptide product of the gene in question functions as a hormone. The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i.e., synthetic or natural oligonucleotides. In further aspects, the present invention provides a cloning vector, which comprises the nucleic acids of the invention; and a bacterial, insect, or a mammalian expression vector, which comprises the nucleic acid molecules of the invention, operatively associated with an expression control sequence. Accordingly, the invention further relates to a bacterial or a mammalian cell transfected or transformed with an appropriate expression vector, and correspondingly, to the use of the above mentioned constructs in the preparation of the modulators of the invention. Also provided are antibodies to the ob polypeptide. Moreover, a method for modulating body weight of a mammal is provided. In specific examples, genes encoding two isoforms of both the murine and human ob polypeptides are provided.

公开(公告)号：US08173793B2

公开(公告)日：2012-05-08

申请号：US12436773

申请日：2009-05-06

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca ,  Margherita Maffei ,  Jeffrey L. Halaas ,  Ketan Gajiwala ,  Stephen K. Burley

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca ,  Margherita Maffei ,  Jeffrey L. Halaas ,  Ketan Gajiwala ,  Stephen K. Burley

IPC分类号： C07H21/04 ,  C12N15/16 ,  C12N15/12

CPC分类号： C07K14/5759 ,  A01K2217/05 ,  A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K16/18 ,  C07K2319/00 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to nucleic acids encoding materials identified herein as modulators of weight. In its broadest aspect, the present invention relates to the elucidation and discovery of nucleotide sequences, and proteins putatively expressed by such nucleotides, that demonstrate the ability to participate in the control of mammalian body weight. The nucleotide sequences in object represent the genes corresponding to the murine and human ob gene, that have been postulated to play a critical role in the regulation of body weight and adiposity. Preliminary data, presented herein, suggests that the polypeptide product of the gene in question functions as a hormone. The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i.e., synthetic or natural oligonucleotides.

摘要翻译： 本发明一般涉及包括哺乳动物和人在内的动物的体重控制，更具体地说，涉及编码本文中鉴定的调节剂的核酸。 在其最广泛的方面，本发明涉及阐明和发现核苷酸序列和由这些核苷酸推定表达的蛋白质，其表现出参与控制哺乳动物体重的能力。 物体中的核苷酸序列代表了对应于鼠和人类ob基因的基因，这些基因被假定在调节体重和肥胖中起关键作用。 本文提供的初步数据表明，所讨论的基因的多肽产物作为激素起作用。 本发明还提供用作分子探针的核酸分子，或用作聚合酶链反应（PCR）扩增的引物，即合成或天然寡核苷酸。

公开(公告)号：US07994301B2

公开(公告)日：2011-08-09

申请号：US12427410

申请日：2009-04-21

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： C07H21/04

CPC分类号： C07K14/5759 ,  A01K2217/05 ,  A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K16/18 ,  C07K2319/00 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： Modulators of weight, including, for example, two isoforms of murine and human ob polypeptides, are provided, as well as diagnostic and therapeutic uses and methods comprising such. Also provided are nucleotide sequences, degenerate variations thereof, and proteins expressed by such.

摘要翻译： 提供重量的调节剂，包括例如鼠和人ob多肽的两种同种型，以及包括这些的诊断和治疗用途和方法。 还提供了核苷酸序列，其简并变异和由其表达的蛋白质。

公开(公告)号：US06734160B2

公开(公告)日：2004-05-11

申请号：US09316393

申请日：1999-05-21

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： A61K3817

CPC分类号： C07K14/5759 ,  A01K2217/05 ,  A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K16/18 ,  C07K2319/00 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of weight, and to the diagnostic and therapeutic uses to which such modulators may be put. In its broadest aspect, the present invention relates to the elucidation and discovery of nucleotide sequences, and proteins putatively expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian body weight. The nucleotide sequences in object represent the genes corresponding to the murine and human ob gene, that have been postulated to play a critical role in the regulation of body weight and adiposity. Preliminary data, presented herein, suggests that the polypeptide product of the gene in question functions as a hormone. The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i.e., synthetic or natural oligonucleotides. In further aspects, the present invention provides a cloning vector, which comprises the nucleic acids of the invention; and a bacterial, insect, or a mammalian expression vector, which comprises the nucleic acid molecules of the invention, operatively associated with an expression control sequence. Accordingly, the invention further relates to a bacterial cell or a mammalian transfected or transformed with an appropriate expression vector, and correspondingly, to the use of the above mentioned constructs in the preparation of the modulators of the invention. Also provided are antibodies to the ob polypeptide. Moreover, a method for modulating body weight of a mammal is provided. In specific examples, genes encoding two isoforms of both the murine and human ob polypeptides are provided.

摘要翻译： 本发明一般涉及包括哺乳动物和人在内的动物的体重控制，更具体地说，涉及本文中鉴定为重量调节剂的材料，以及可以将这些调节剂置于其上的诊断和治疗用途。 在其最广泛的方面，本发明涉及阐明和发现核苷酸序列和由这些核苷酸或其简并变异体推测表达的蛋白质，其表现出参与控制哺乳动物体重的能力。 物体中的核苷酸序列代表了对应于鼠和人类ob基因的基因，这些基因被假定在调节体重和肥胖中起关键作用。 本文提供的初步数据表明，所讨论的基因的多肽产物作为激素起作用。 本发明还提供用作分子探针的核酸分子，或用作聚合酶链反应（PCR）扩增的引物，即合成或天然寡核苷酸。 在另外的方面，本发明提供了一种克隆载体，其包含本发明的核酸; 和细菌，昆虫或哺乳动物表达载体，其包含与表达调控序列有效连接的本发明的核酸分子。 因此，本发明还涉及用合适的表达载体转染或转化的细菌细胞或哺乳动物，并且相应地涉及在制备本发明的调节剂中使用上述构建体。 还提供了针对ob多肽的抗体。 此外，提供了一种用于调节哺乳动物体重的方法。 在具体实例中，提供编码鼠和人类ob多态的两种同种型的基因。

公开(公告)号：US6124439A

公开(公告)日：2000-09-26

申请号：US488214

申请日：1995-06-07

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K14/575 ,  C07K16/18 ,  C07K16/26 ,  C12N15/12 ,  C12Q1/68 ,  C07K16/00

CPC分类号： A61K8/64 ,  A61Q19/06 ,  C07K14/5759 ,  C07K16/18 ,  C07K16/26 ,  C12Q1/6883 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K2317/34 ,  C07K2319/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S435/975 ,  Y10S530/864

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of body weight, and to diagnostic and therapeutic uses of such modulators. In its broadest aspect, the present invention relates to nucleotide sequences corresponding to the murine and human OB gene, and two isoforms thereof, and proteins expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian body weight and that have been postulated to play a critical role in the regulation of body weight and adiposity. The present invention further provides nucleic acid molecules for use as molecular probes or as primers for polymerase chain reaction (PCR) amplification. In further aspects, the present invention provides cloning vectors and mammalian expression vectors comprising the nucleic acid molecules of the invention. The invention further relates to host cells transfected or transformed with an appropriate expression vector and to their use in the preparation of the modulators of the invention. Also provided are antibodies to the OB polypeptide. Moreover, a method for modulating body weight of a mammal is provided.

摘要翻译： 本发明一般涉及包括哺乳动物和人在内的动物的体重控制，更具体地说涉及本文中鉴定的作为体重调节剂的物质，以及这些调节剂的诊断和治疗用途。 在其最广泛的方面，本发明涉及对应于鼠和人OB基因及其两种同工型的核苷酸序列，以及由这些核苷酸或其简并变异表达的蛋白质，其表现出参与哺乳动物体重控制的能力 并假定在调节体重和肥胖方面发挥关键作用。 本发明还提供用作分子探针或用作聚合酶链反应（PCR）扩增的引物的核酸分子。 在另外的方面，本发明提供了包含本发明的核酸分子的克隆载体和哺乳动物表达载体。 本发明进一步涉及用合适的表达载体转染或转化的宿主细胞及其在制备本发明调节剂中的用途。 还提供了针对OB多肽的抗体。 此外，提供了一种用于调节哺乳动物体重的方法。

公开(公告)号：US6048837A

公开(公告)日：2000-04-11

申请号：US485942

申请日：1995-06-07

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K14/575 ,  C07K16/18 ,  C07K16/26 ,  C12N15/12 ,  C12Q1/68

CPC分类号： A61K8/64 ,  A61Q19/06 ,  C07K14/5759 ,  C07K16/18 ,  C07K16/26 ,  C12Q1/6883 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K2317/34 ,  C07K2319/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/844 ,  Y10S514/866 ,  Y10S514/909

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of body weight, and to diagnostic and therapeutic uses of such modulators. In its broadest aspect, the present invention relates to nucleotide sequences corresponding to the murine and human OB gene, and two isoforms thereof, and proteins expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian body weight and that have been postulated to play a critical role in the regulation of body weight and adiposity. The present invention further provides nucleic acid molecules for use as molecular probes or as primers for polymerase chain reaction (PCR) amplification. In further aspects, the present invention provides cloning vectors and mammalian expression vectors comprising the nucleic acid molecules of the invention. The invention further relates to host cells transfected or transformed with an appropriate expression vector and to their use in the preparation of the modulators of the invention. Also provided are antibodies to the OB polypeptide. Moreover, a method for modulating body weight of a mammal is provided.

摘要翻译： 本发明一般涉及包括哺乳动物和人在内的动物的体重控制，更具体地说涉及本文中鉴定的作为体重调节剂的物质，以及这些调节剂的诊断和治疗用途。 在其最广泛的方面，本发明涉及对应于鼠和人OB基因及其两种同工型的核苷酸序列，以及由这些核苷酸或其简并变异表达的蛋白质，其表现出参与哺乳动物体重控制的能力 并假定在调节体重和肥胖方面发挥关键作用。 本发明还提供用作分子探针或用作聚合酶链反应（PCR）扩增的引物的核酸分子。 在另外的方面，本发明提供了包含本发明的核酸分子的克隆载体和哺乳动物表达载体。 本发明进一步涉及用合适的表达载体转染或转化的宿主细胞及其在制备本发明调节剂中的用途。 还提供了针对OB多肽的抗体。 此外，提供了一种用于调节哺乳动物体重的方法。

公开(公告)号：US6001968A

公开(公告)日：1999-12-14

申请号：US292345

申请日：1994-08-17

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： A61K8/64 ,  A61K38/00 ,  A61K38/17 ,  A61K48/00 ,  A61Q19/06 ,  C07K14/47 ,  C07K14/575 ,  C07K16/18 ,  C12N1/19 ,  C12N1/21 ,  C12N5/02 ,  C12N5/10 ,  C12N5/16 ,  C12N15/12 ,  C12N15/16 ,  C12Q1/68 ,  G01N33/53 ,  C07K1/00

CPC分类号： C07K14/5759 ,  A61K8/64 ,  A61Q19/06 ,  C07K16/18 ,  C12Q1/6883 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of body weight, and to diagnostic and therapeutic uses of such modulators. In its broadest aspect, the present invention relates to nucleotide sequences corresponding to the murine and human OB gene, and two isoforms thereof, and proteins expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability co participate in the control of mammalian body weight and that have been postulated to play a critical role in the regulation of body weight and adiposity. The present invention further provides nucleic acid molecules for use as molecular probes or as primers for polymerase chain reaction (PCR) amplification. In further aspects, the present invention provides cloning vectors and mammalian expression vectors comprising the nucleic acid molecules of the invention. The invention further relates to host cells transfected or transformed with an appropriate expression vector and to their use in the preparation of the modulators of the invention. Also provided are antibodies to the OB polypeptide. Moreover, a method for modulating body weight of a mammal is provided.

摘要翻译： 本发明一般涉及包括哺乳动物和人在内的动物的体重控制，更具体地说涉及本文中鉴定的作为体重调节剂的物质，以及这些调节剂的诊断和治疗用途。 在其最广泛的方面，本发明涉及对应于鼠和人OB基因及其两种同工型的核苷酸序列，以及由这些核苷酸或其简并变异表达的蛋白质，其表现出参与控制哺乳动物体重的能力 并假定在调节体重和肥胖方面发挥关键作用。 本发明还提供用作分子探针或用作聚合酶链反应（PCR）扩增的引物的核酸分子。 在另外的方面，本发明提供了包含本发明的核酸分子的克隆载体和哺乳动物表达载体。 本发明进一步涉及用合适的表达载体转染或转化的宿主细胞及其在制备本发明调节剂中的用途。 还提供了针对OB多肽的抗体。 此外，提供了一种用于调节哺乳动物体重的方法。

公开(公告)号：US08631272B2

公开(公告)日：2014-01-14

申请号：US13040277

申请日：2011-03-04

申请人： Vijayan Prabhakaran ,  Yiying Zhang

发明人： Vijayan Prabhakaran ,  Yiying Zhang

IPC分类号： G06F7/06

CPC分类号： G06F11/2082 ,  G06F3/061 ,  G06F3/0614 ,  G06F3/064 ,  G06F3/067 ,  G06F3/0689 ,  G06F11/1662 ,  G06F11/2087

摘要： A duplicate-aware disk array (DADA) leaves duplicated content on the disk array largely unmodified, instead of removing duplicated content, and then uses these duplicates to improve system performance, reliability, and availability of the disk array. Several implementations disclosed herein are directed to the selection of one duplicate from among a plurality of duplicates to act as the proxy for the other duplicates found in the disk array. Certain implementations disclosed herein are directed to scrubbing latent sector errors (LSEs) on duplicate-aware disk arrays. Other implementations are directed to disk reconstruction/recovery on duplicate-aware disk arrays. Yet other implementations are directed to load balancing on duplicate-aware disk arrays.

摘要翻译： 重复感知磁盘阵列（DADA）将磁盘阵列上重复的内容大部分未修改，而不是删除重复的内容，然后使用这些重复来提高磁盘阵列的系统性能，可靠性和可用性。 本文中公开的几个实施例涉及从多个复制品中选择一个副本，以用作在盘阵列中发现的其他重复的代理。 本文公开的某些实施方式涉及擦除重复感知磁盘阵列上的潜在扇区错误（LSE）。 其他实现针对重复感知磁盘阵列上的磁盘重建/恢复。 还有其他实现涉及重复感知磁盘阵列上的负载平衡。

公开(公告)号：US07521258B2

公开(公告)日：2009-04-21

申请号：US10780295

申请日：2004-02-17

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/536 ,  G01N33/537 ,  G01N33/543 ,  A61K39/395 ,  A61K39/44

CPC分类号： C07K14/5759 ,  A01K2217/05 ,  A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K16/18 ,  C07K2319/00 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： Modulators of weight, including, for example, two isoforms of murine and human ob polypeptides, are provided, as well as diagnostic and therapeutic uses and methods comprising such. Also provided are nucleotide sequences, degenerate variations thereof, and proteins expressed by such. Such materials are useful, for example, as molecular probes, as primers for polymerase chain reaction (PCR) amplification, as well as for the generation of antibodies. Also provided are vectors comprising such nucleotide sequences, including bacterial, insect, and mammalian expression vectors, which nucleotide sequences are operatively associated with an expression control sequence. Cells transformed or transfected with such vectors, and the use of such in the preparation of disclosed modulators, are also provided.

摘要翻译： 提供重量的调节剂，包括例如鼠和人ob多肽的两种同种型，以及包括这些的诊断和治疗用途和方法。 还提供了核苷酸序列，其简并变异和由其表达的蛋白质。 这样的材料可用作例如分子探针，作为聚合酶链式反应（PCR）扩增的引物，以及产生抗体。 还提供了包含这样的核苷酸序列的载体，包括细菌，昆虫和哺乳动物表达载体，该核苷酸序列与表达控制序列可操作地相关联。 还提供了用这种载体转化或转染的细胞，以及其在制备所公开的调节剂中的用途。

公开(公告)号：US06703493B1

公开(公告)日：2004-03-09

申请号：US09204730

申请日：1998-12-03

申请人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

发明人： Jeffrey M. Friedman ,  Yiying Zhang ,  Ricardo Proenca

IPC分类号： C07K1447

CPC分类号： C07K14/5759 ,  A01K2217/05 ,  A61K8/64 ,  A61K38/00 ,  A61K48/00 ,  A61Q19/06 ,  C07K16/18 ,  C07K2319/00 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y10S514/866 ,  Y10S514/909 ,  Y10S930/12 ,  Y10S930/14

摘要： The present invention relates generally to the control of body weight of animals including mammals and humans, and more particularly to materials identified herein as modulators of weight, and to the diagnostic and therapeutic uses to which such modulators may be put. In its broadest aspect, the present invention relates to the elucidation and discovery of nucleotide sequences, and proteins putatively expressed by such nucleotides or degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian body weight. The nucleotide sequences in object represent the genes corresponding to the murine and human ob gene, that have been postulated to play a critical role in the regulation of body weight and adiposity. Preliminary data, presented herein, suggests that the polypeptide product of the gene in question functions as a hormone. The present invention further provides nucleic acid molecules for use as molecular probes, or as primers for polymerase chain reaction (PCR) amplification, i.e., synthetic or natural oligonucleotides. In further aspects, the present invention provides a cloning vector, which comprises the nucleic acids of the invention; and a bacterial, insect, or a mammalian expression vector, which comprises the nucleic acid molecules of the invention, operatively associated with an expression control sequence. Accordingly, the invention further relates to a bacterial cell or a mammalian transfected or transformed with an appropriate expression vector, and correspondingly, to the use of the above mentioned constructs in the preparation of the modulators of the invention. Also provided are antibodies to the ob polypeptide. Moreover, a method for modulating body weight of a mammal is provided. In specific examples, genes encoding two isoforms of both the murine and human ob polypeptides are provided.

摘要翻译： 本发明一般涉及包括哺乳动物和人在内的动物的体重控制，更具体地说，涉及本文中鉴定为重量调节剂的材料，以及可以将这些调节剂置于其上的诊断和治疗用途。 在其最广泛的方面，本发明涉及阐明和发现核苷酸序列和由这些核苷酸或其简并变异体推测表达的蛋白质，其表现出参与控制哺乳动物体重的能力。 物体中的核苷酸序列代表了对应于鼠和人类ob基因的基因，这些基因被假定在调节体重和肥胖中起关键作用。 本文提供的初步数据表明，所讨论的基因的多肽产物作为激素起作用。 本发明还提供用作分子探针的核酸分子，或用作聚合酶链反应（PCR）扩增的引物，即合成或天然寡核苷酸。 在另外的方面，本发明提供了一种克隆载体，其包含本发明的核酸; 和细菌，昆虫或哺乳动物表达载体，其包含与表达调控序列有效连接的本发明的核酸分子。 因此，本发明还涉及用合适的表达载体转染或转化的细菌细胞或哺乳动物，并且相应地涉及在制备本发明的调节剂中使用上述构建体。 还提供了针对ob多肽的抗体。 此外，提供了一种用于调节哺乳动物体重的方法。 在具体实例中，提供编码鼠和人类ob多态的两种同种型的基因。